Detailed information of regulator
Summary
| Regulator ID | REG0281  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Biosynthesis of secondary metabolites (map01110); 2-Oxocarboxylic acid metabolism (map01210); Lysine biosynthesis (map00300) |
| Related T6SS | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (383 a.a.); Nucleotide sequence (1149 bp) |
| Description | Transcriptional activator,identified to bind to the promoters of both aphA and luxR. |
| Reference |
External database links
| Locus tag (Gene) | V12G01_02325 (HP) |
| Coordinates (Strand) | 115263..116411 (+) |
| NCBI ID | 91190993 |
| GenBank | AAPS01000004 |
| Uniprot ID | UPI0000D52D3F |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map01100; map01110; map01210; map00300 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| V12G01-21538 (luxR) promoter | TGAGTGTAA TGGTGCATATGCACAAGGTCAA | |
| V12G01-14830 (aphA) promoter | CACAAGTTAACACTATTGATTATTATGTTAGTTTT |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Aminotran_1_2 | PF00155.23 | 1.2e-31 | 26..375 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-382 | MEIAQSLQQTKSSYIREILAAASDKNVISLAGGLPDEKTFPIDLMKPTLENLANMPEVFQYGATAGYAPLLNFFTDYYQLPESHLAMITTGSQQGLDLIARAYVNPGDKVVMEAPSYLGAMQVFGLVQANIVTVSQTECGPNLEELEQCFATQQPKMFYAVPDFHNPTGVCWALETRQKVAELCIQYDVAFIEDAPYRELRFSGEALPLVSDFCPQHSIVLRSFSKIASPGLRIGVVTGKSSYLEPLIKIKQGADLHSSIPMQALLYGLLQHDNFETHISTICALYKSRYDVMFAELQKQLPESCVVKPVDGGMFVWVEIPECDTFELAKNLLANRVAVVPSPVFYPEGSKVKAALRLNFTNATPEELTTAVSRLADVLNTL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9737 | - | - |
Protein sequence of regulator: 383 a.a. .
>REG0281 AAPS01000004:115263-116411 [Vibrio alginolyticus 12G01]
MEIAQSLQQTKSSYIREILAAASDKNVISLAGGLPDEKTFPIDLMKPTLENLANMPEVFQYGATAGYAPLLNFFTDYYQL
PESHLAMITTGSQQGLDLIARAYVNPGDKVVMEAPSYLGAMQVFGLVQANIVTVSQTECGPNLEELEQCFATQQPKMFYA
VPDFHNPTGVCWALETRQKVAELCIQYDVAFIEDAPYRELRFSGEALPLVSDFCPQHSIVLRSFSKIASPGLRIGVVTGK
SSYLEPLIKIKQGADLHSSIPMQALLYGLLQHDNFETHISTICALYKSRYDVMFAELQKQLPESCVVKPVDGGMFVWVEI
PECDTFELAKNLLANRVAVVPSPVFYPEGSKVKAALRLNFTNATPEELTTAVSRLADVLNTL*
MEIAQSLQQTKSSYIREILAAASDKNVISLAGGLPDEKTFPIDLMKPTLENLANMPEVFQYGATAGYAPLLNFFTDYYQL
PESHLAMITTGSQQGLDLIARAYVNPGDKVVMEAPSYLGAMQVFGLVQANIVTVSQTECGPNLEELEQCFATQQPKMFYA
VPDFHNPTGVCWALETRQKVAELCIQYDVAFIEDAPYRELRFSGEALPLVSDFCPQHSIVLRSFSKIASPGLRIGVVTGK
SSYLEPLIKIKQGADLHSSIPMQALLYGLLQHDNFETHISTICALYKSRYDVMFAELQKQLPESCVVKPVDGGMFVWVEI
PECDTFELAKNLLANRVAVVPSPVFYPEGSKVKAALRLNFTNATPEELTTAVSRLADVLNTL*
Nucleotide sequence of regulator: 1149 bp .
>REG0281 AAPS01000004:115263-116411 [Vibrio alginolyticus 12G01]
ATGGAAATCGCACAGTCATTACAACAAACTAAGTCATCCTACATCCGAGAAATCCTCGCAGCAGCGAGCGATAAGAACGT
CATTTCTCTTGCAGGTGGCTTACCGGACGAAAAAACCTTCCCGATCGACTTAATGAAACCGACGCTCGAAAACCTCGCTA
ATATGCCAGAGGTGTTTCAATACGGCGCAACAGCAGGCTACGCCCCGCTACTTAACTTTTTTACCGATTATTACCAGCTG
CCAGAATCTCATTTGGCGATGATCACAACGGGCTCTCAGCAAGGGCTAGATTTGATTGCGCGTGCGTACGTGAATCCGGG
CGATAAAGTGGTGATGGAAGCACCGAGCTACCTTGGTGCTATGCAAGTATTCGGTCTAGTGCAAGCCAACATCGTGACCG
TTTCACAAACCGAGTGTGGCCCAAACCTTGAAGAACTCGAACAATGCTTTGCCACGCAGCAACCAAAAATGTTCTACGCC
GTACCCGATTTTCACAACCCAACGGGCGTATGCTGGGCGCTAGAGACTCGCCAAAAAGTTGCCGAGCTTTGTATACAATA
CGACGTGGCGTTCATTGAAGACGCACCATACCGCGAGCTGCGTTTTTCTGGTGAAGCGCTTCCACTGGTTTCGGACTTCT
GCCCACAGCACTCCATCGTATTGCGCTCTTTCTCCAAAATAGCTTCACCAGGCTTGCGTATTGGTGTAGTTACGGGCAAA
AGCAGCTACCTTGAGCCACTTATCAAAATTAAACAAGGTGCCGATCTGCACTCTAGTATTCCTATGCAAGCTTTGCTGTA
TGGCCTGCTGCAACATGACAACTTCGAAACGCACATTTCAACCATCTGTGCGCTTTACAAATCACGTTACGATGTCATGT
TTGCTGAGCTGCAAAAACAGCTGCCAGAAAGTTGCGTAGTCAAACCCGTCGATGGTGGCATGTTCGTTTGGGTCGAGATT
CCAGAATGCGACACCTTCGAACTGGCGAAAAACCTTCTCGCAAACAGGGTTGCTGTAGTGCCGAGCCCGGTCTTTTATCC
AGAAGGTTCAAAGGTAAAAGCCGCTCTGCGTCTTAACTTTACCAATGCCACACCAGAAGAACTGACGACAGCTGTCTCTC
GACTCGCGGATGTCCTCAACACGTTGTAA
ATGGAAATCGCACAGTCATTACAACAAACTAAGTCATCCTACATCCGAGAAATCCTCGCAGCAGCGAGCGATAAGAACGT
CATTTCTCTTGCAGGTGGCTTACCGGACGAAAAAACCTTCCCGATCGACTTAATGAAACCGACGCTCGAAAACCTCGCTA
ATATGCCAGAGGTGTTTCAATACGGCGCAACAGCAGGCTACGCCCCGCTACTTAACTTTTTTACCGATTATTACCAGCTG
CCAGAATCTCATTTGGCGATGATCACAACGGGCTCTCAGCAAGGGCTAGATTTGATTGCGCGTGCGTACGTGAATCCGGG
CGATAAAGTGGTGATGGAAGCACCGAGCTACCTTGGTGCTATGCAAGTATTCGGTCTAGTGCAAGCCAACATCGTGACCG
TTTCACAAACCGAGTGTGGCCCAAACCTTGAAGAACTCGAACAATGCTTTGCCACGCAGCAACCAAAAATGTTCTACGCC
GTACCCGATTTTCACAACCCAACGGGCGTATGCTGGGCGCTAGAGACTCGCCAAAAAGTTGCCGAGCTTTGTATACAATA
CGACGTGGCGTTCATTGAAGACGCACCATACCGCGAGCTGCGTTTTTCTGGTGAAGCGCTTCCACTGGTTTCGGACTTCT
GCCCACAGCACTCCATCGTATTGCGCTCTTTCTCCAAAATAGCTTCACCAGGCTTGCGTATTGGTGTAGTTACGGGCAAA
AGCAGCTACCTTGAGCCACTTATCAAAATTAAACAAGGTGCCGATCTGCACTCTAGTATTCCTATGCAAGCTTTGCTGTA
TGGCCTGCTGCAACATGACAACTTCGAAACGCACATTTCAACCATCTGTGCGCTTTACAAATCACGTTACGATGTCATGT
TTGCTGAGCTGCAAAAACAGCTGCCAGAAAGTTGCGTAGTCAAACCCGTCGATGGTGGCATGTTCGTTTGGGTCGAGATT
CCAGAATGCGACACCTTCGAACTGGCGAAAAACCTTCTCGCAAACAGGGTTGCTGTAGTGCCGAGCCCGGTCTTTTATCC
AGAAGGTTCAAAGGTAAAAGCCGCTCTGCGTCTTAACTTTACCAATGCCACACCAGAAGAACTGACGACAGCTGTCTCTC
GACTCGCGGATGTCCTCAACACGTTGTAA