Detailed information of regulator
Summary
| Regulator ID | REG0284  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (304 a.a.); Nucleotide sequence (912 bp) |
| Description | LysR family MetR,identified to bind to the promoters of both aphA and luxR. |
| Reference |
External database links
| Locus tag (Gene) | V12G01_16177 (MetR) |
| Coordinates (Strand) | 58420..59331 (-) |
| NCBI ID | 91188901 |
| GenBank | AAPS01000023 |
| Uniprot ID | UPI0000D52907 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| V12G01-21538 (luxR) promoter | TGAGTGTAA TGGTGCATATGCACAAGGTCAA | |
| V12G01-14830 (aphA) promoter | CACAAGTTAACACTATTGATTATTATGTTAGTTTT |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LysR_substrate | PF03466.22 | 3.8e-42 | 88..290 |
| HTH_1 | PF00126.29 | 3e-17 | 4..63 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-303 | MIELKHLRTLTSLRDTGSLTATATALHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLAEDILPKLARAENDLASLKEDVNGRLHMAIECHSCFQWLMPALREYQLAWPSVTLDFSSGFGFEPLPALLAGELDLVITSDIQPRSEVHYEPLFDFEMRLITATNHPLAEKEIIDPQDLADQTMLSYPVQKQRLDVVKHFLQPAGIEPAKWKQADNTLMLVQMVSAGLGVAALPNWAISEFSRQGLITSKPFGQGLWRRLFAATRHSEKEKRYLQAFFATARLQCKSHLDGIKVA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.6510 | - | - |
Protein sequence of regulator: 304 a.a. .
>REG0284 AAPS01000023:c59331-58420 [Vibrio alginolyticus 12G01]
MIELKHLRTLTSLRDTGSLTATATALHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLAEDILPKLARAE
NDLASLKEDVNGRLHMAIECHSCFQWLMPALREYQLAWPSVTLDFSSGFGFEPLPALLAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITATNHPLAEKEIIDPQDLADQTMLSYPVQKQRLDVVKHFLQPAGIEPAKWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSKPFGQGLWRRLFAATRHSEKEKRYLQAFFATARLQCKSHLDGIKVA*
MIELKHLRTLTSLRDTGSLTATATALHLTQSALSHQIKDLEARIGGQLFLRKTRPVKFTSEGEILLRLAEDILPKLARAE
NDLASLKEDVNGRLHMAIECHSCFQWLMPALREYQLAWPSVTLDFSSGFGFEPLPALLAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITATNHPLAEKEIIDPQDLADQTMLSYPVQKQRLDVVKHFLQPAGIEPAKWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLITSKPFGQGLWRRLFAATRHSEKEKRYLQAFFATARLQCKSHLDGIKVA*
Nucleotide sequence of regulator: 912 bp .
>REG0284 AAPS01000023:c59331-58420 [Vibrio alginolyticus 12G01]
ATGATAGAGCTAAAACATTTGCGGACACTCACTTCGCTTCGAGACACTGGCTCTCTAACGGCGACAGCGACCGCTTTGCA
TCTCACTCAGTCGGCACTGTCTCATCAGATCAAAGATTTGGAGGCGCGAATTGGAGGGCAACTGTTCCTGAGAAAGACTC
GTCCGGTGAAGTTCACTTCAGAGGGAGAAATCCTATTGCGTCTTGCAGAAGATATCCTTCCGAAACTCGCAAGAGCAGAA
AATGATTTAGCGAGCTTGAAAGAGGACGTCAATGGCCGATTACATATGGCCATTGAATGTCACTCTTGCTTCCAATGGCT
AATGCCTGCACTTAGGGAGTACCAACTTGCTTGGCCTAGCGTAACATTGGACTTTTCCTCTGGTTTTGGCTTTGAACCAC
TCCCAGCTCTATTGGCAGGCGAACTCGATCTCGTTATTACTTCCGATATCCAACCTCGATCTGAAGTCCACTACGAGCCT
CTATTCGACTTTGAAATGCGTTTAATTACCGCAACCAACCACCCATTGGCGGAAAAAGAAATTATTGATCCGCAAGATCT
CGCAGATCAAACCATGCTCTCTTATCCTGTTCAAAAACAAAGGCTAGACGTCGTAAAACACTTTCTTCAGCCAGCAGGAA
TAGAACCTGCGAAATGGAAGCAAGCCGACAACACTCTCATGTTAGTTCAGATGGTCTCTGCTGGATTGGGGGTGGCCGCA
CTACCCAATTGGGCGATTTCTGAATTTTCTCGCCAAGGTCTTATTACCAGTAAACCTTTCGGCCAAGGCTTATGGCGTCG
CTTGTTTGCAGCAACGCGTCACTCAGAGAAAGAGAAGCGTTACTTACAAGCTTTTTTCGCAACCGCGAGGCTACAATGTA
AAAGCCACCTTGACGGAATCAAGGTGGCTTAA
ATGATAGAGCTAAAACATTTGCGGACACTCACTTCGCTTCGAGACACTGGCTCTCTAACGGCGACAGCGACCGCTTTGCA
TCTCACTCAGTCGGCACTGTCTCATCAGATCAAAGATTTGGAGGCGCGAATTGGAGGGCAACTGTTCCTGAGAAAGACTC
GTCCGGTGAAGTTCACTTCAGAGGGAGAAATCCTATTGCGTCTTGCAGAAGATATCCTTCCGAAACTCGCAAGAGCAGAA
AATGATTTAGCGAGCTTGAAAGAGGACGTCAATGGCCGATTACATATGGCCATTGAATGTCACTCTTGCTTCCAATGGCT
AATGCCTGCACTTAGGGAGTACCAACTTGCTTGGCCTAGCGTAACATTGGACTTTTCCTCTGGTTTTGGCTTTGAACCAC
TCCCAGCTCTATTGGCAGGCGAACTCGATCTCGTTATTACTTCCGATATCCAACCTCGATCTGAAGTCCACTACGAGCCT
CTATTCGACTTTGAAATGCGTTTAATTACCGCAACCAACCACCCATTGGCGGAAAAAGAAATTATTGATCCGCAAGATCT
CGCAGATCAAACCATGCTCTCTTATCCTGTTCAAAAACAAAGGCTAGACGTCGTAAAACACTTTCTTCAGCCAGCAGGAA
TAGAACCTGCGAAATGGAAGCAAGCCGACAACACTCTCATGTTAGTTCAGATGGTCTCTGCTGGATTGGGGGTGGCCGCA
CTACCCAATTGGGCGATTTCTGAATTTTCTCGCCAAGGTCTTATTACCAGTAAACCTTTCGGCCAAGGCTTATGGCGTCG
CTTGTTTGCAGCAACGCGTCACTCAGAGAAAGAGAAGCGTTACTTACAAGCTTTTTTCGCAACCGCGAGGCTACAATGTA
AAAGCCACCTTGACGGAATCAAGGTGGCTTAA