SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015467
Component protein type	TssJ
T6SS ID (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (152 a.a.); Nucleotide sequence (456 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS31170
Coordinate (Strand)	1444661..1445116 (+)
NCBI ID	491518302
RefSeq	NZ_CH902589
Uniprot ID	A0A0H0YI78_VIBAL
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.3e-38	31..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MKKFYQ
Transmembrane helix	7-26	YILLLISIALFGCSAANLVV
Outside	27-151	DPYSDLEIAASHNINPDSNGRPSPVVVYVFELTSNTIFESQDFFSIYEESEKVLGPDLVNKYEVSLTPGQKETYQASMSPKTEYLGIVVAFRDIENSNWRQVIKVDKTGYNTYQVLLEDLSLVVK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9646	pos: 18-19. LFG-CS.	MKKFYQYILLLISIALFG

Protein sequence: 152 a.a.

						>T6CP015467 NZ_CH902589:1444661-1445116 [Vibrio alginolyticus 12G01] [TssJ]
MKKFYQYILLLISIALFGCSAANLVVDPYSDLEIAASHNINPDSNGRPSPVVVYVFELTSNTIFESQDFFSIYEESEKVL
GPDLVNKYEVSLTPGQKETYQASMSPKTEYLGIVVAFRDIENSNWRQVIKVDKTGYNTYQVLLEDLSLVVK*					

Nucleotide sequence: 456 bp

						>T6CP015467 NZ_CH902589:1444661-1445116 [Vibrio alginolyticus 12G01] [TssJ]
ATGAAAAAGTTTTACCAATATATTTTGTTATTAATTTCAATAGCTTTATTTGGGTGCTCAGCAGCGAACCTAGTTGTTGA
TCCATACTCAGATTTAGAAATAGCAGCAAGTCATAACATTAATCCTGATTCAAACGGCAGGCCTTCTCCTGTAGTGGTGT
ATGTATTTGAATTAACGTCAAATACCATTTTCGAAAGCCAGGACTTTTTTTCTATTTATGAAGAAAGTGAAAAAGTCTTA
GGACCTGATTTAGTGAATAAATATGAAGTTTCATTAACTCCAGGTCAGAAAGAAACATATCAAGCAAGTATGTCTCCAAA
GACAGAATATTTAGGCATTGTCGTAGCGTTTCGAGATATTGAAAACTCAAATTGGCGACAAGTCATTAAAGTGGATAAGA
CCGGTTACAACACATATCAAGTATTGTTAGAAGATCTGTCTTTGGTCGTTAAATAA					