SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015480
Component protein type	TssG
T6SS ID (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (322 a.a.); Nucleotide sequence (966 bp)
Reference	[1] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539 [2] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	V12G01_RS31235
Coordinate (Strand)	1460588..1461553 (+)
NCBI ID	491518332
RefSeq	NZ_CH902589
Uniprot ID	A0A2I3CRM1_VIBAX, A0A0P7EM68_VIBAL
KEGG ID	vag:N646_4400
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.3e-65	11..306

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-321	MSAEQFYRELKQHPLFEAIHLIEHEVLQAEAQIGTDALPKHEKLNLKVNPSLGFENAQLCELKNIDDSKLEIETNLIGLTGEQGVLPQHYSELALHRQKEGDTAMVDFYDIFNHRLLSLYYRCWQLSQLTVQSRAHAQGARSPLESCKSALTGKGSSLSRHYGGVFASPVRSKGGLKSILECLSGCQVKVHEFQGQWLHLTKEEQTRLASRTMPEGQFAQLGLGASLGSKAWNINAGTTIEFLPKDKQLVSKLLPNQPNIAIIKNAAYELIGKHKQLKWQLTTKHSLLPQACLSKQQGQLGIGSVLKKHERTKDRTITITI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9961	-	-

Protein sequence: 322 a.a.

						>T6CP015480 NZ_CH902589:1460588-1461553 [Vibrio alginolyticus 12G01] [TssG]
MSAEQFYRELKQHPLFEAIHLIEHEVLQAEAQIGTDALPKHEKLNLKVNPSLGFENAQLCELKNIDDSKLEIETNLIGLT
GEQGVLPQHYSELALHRQKEGDTAMVDFYDIFNHRLLSLYYRCWQLSQLTVQSRAHAQGARSPLESCKSALTGKGSSLSR
HYGGVFASPVRSKGGLKSILECLSGCQVKVHEFQGQWLHLTKEEQTRLASRTMPEGQFAQLGLGASLGSKAWNINAGTTI
EFLPKDKQLVSKLLPNQPNIAIIKNAAYELIGKHKQLKWQLTTKHSLLPQACLSKQQGQLGIGSVLKKHERTKDRTITIT
I*					

Nucleotide sequence: 966 bp

						>T6CP015480 NZ_CH902589:1460588-1461553 [Vibrio alginolyticus 12G01] [TssG]
ATGAGCGCGGAACAATTTTATCGAGAATTAAAGCAACATCCATTGTTTGAAGCGATTCATCTTATTGAACACGAAGTCCT
GCAAGCAGAGGCGCAAATAGGAACAGATGCGTTACCGAAGCATGAAAAGCTAAACTTAAAGGTTAATCCATCATTAGGTT
TTGAAAATGCTCAGCTTTGTGAACTGAAAAATATAGATGACAGCAAGTTAGAGATTGAGACCAATTTAATTGGCCTAACG
GGTGAGCAAGGTGTGCTTCCCCAGCACTATAGTGAATTAGCTTTACATCGCCAAAAAGAAGGCGATACGGCTATGGTGGA
CTTCTACGATATTTTCAATCATCGCTTATTATCTCTTTATTATCGATGTTGGCAATTGAGTCAGCTGACTGTTCAGTCTC
GTGCGCATGCGCAGGGCGCGCGTTCTCCGTTAGAAAGCTGTAAATCGGCATTAACTGGGAAAGGGAGTTCACTGTCCCGG
CACTATGGTGGTGTGTTTGCTTCACCAGTGAGAAGTAAAGGTGGACTAAAAAGTATTCTTGAATGTTTATCTGGATGCCA
AGTAAAAGTTCATGAATTTCAAGGACAATGGTTACACCTTACGAAAGAAGAGCAAACACGCCTTGCATCCCGAACTATGC
CTGAAGGGCAGTTTGCTCAACTTGGTTTAGGAGCGTCTTTGGGTTCGAAAGCATGGAACATCAACGCGGGGACAACAATA
GAATTTCTGCCAAAAGATAAACAGCTAGTTAGTAAATTGCTTCCTAACCAACCCAATATAGCAATCATTAAGAACGCTGC
TTATGAGTTGATTGGAAAACATAAACAACTGAAGTGGCAGCTCACCACCAAACATAGTTTGCTACCACAAGCTTGTTTAT
CGAAACAGCAGGGGCAGTTAGGTATTGGTTCTGTGCTTAAAAAACACGAACGTACAAAAGATCGAACTATAACAATTACT
ATTTAA					