Detailed information of component protein


Summary

Component ID T6CP015470
Component protein type TssM
T6SS ID (Type) T6SS00939 experimental (Type i5)
Strain Vibrio alginolyticus 12G01
Replicon chromosome
Sequence Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) V12G01_RS31185
Coordinate (Strand) 1447784..1451302 (+)
NCBI ID 491518311
RefSeq NZ_CH902589
Uniprot ID UPI0000D54039
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 2.6e-86 193..450
IcmF-related PF06761.14 2.5e-75 503..803
IcmF_C PF06744.14 1.5e-18 1049..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MRIKEIGRIFTQ
Transmembrane helix 13-32 RWFLGLIGVAACSIFIWVVG
Outside 33-46 PLIAIAGYEPLKSD
Transmembrane helix 47-64 FQRLVTILVLVLLWAIFN
Inside 65-445 VTKQHKQKVKEDESIQTLLEVDSLSDKEAASEIDLMRERIEQAVKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLGDSLGSDSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASVMSQTRTERSLHARAIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDARMLRILETEDDLDKRALIFEFPKQLRVLQANLDEFLAEIFAQNSFEEPALIRGVFMLSSVQEGLPVDKLMNESTNGLGLNRLALAKSQTGSHSYFVKNLFDKVIFKEQLLGTVNRHYQKQSGWMRR
Transmembrane helix 446-463 GVYIGCTTLLLGASALWF
Outside 464-1172 MSYKWNSQLIVDTNEQVSHIDAMIGAQSLDFEADIVSAVETLDRLMTLPLGKQSDYGDADSVKRFGLYQGDKVSQAANNAYSDALTQYYAPLLLDSLVSEMESNQQHREYLYETLKTYLMLFNPDKYQQDEVLAWFNFYFERQYPGELNADLRRRLLQHTQYLLGNDGQGFTYNTAAVTSAREVLTQMSLPERAYQRMKIQFSKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKSLMEENWVYGDDLKAHEIDQDSAIQGVQSRYYQDYVNEWKTLIEDIQLKQAPSLELATEQARVLSGVERPIESLLRAIQKEVALSKVSLSENQKAASEVAGKVAKVKFSNTADRLDMYLPEENGFNVALPGKEVESHFSEILRLGEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKELLPRPFNQWLGELSQESVKFAESGSKDHLNQLWVTQVVRPYQRTIAGRYPFEPNASREVRLKDFKRFFGYGGTLDAFFQEYLEPFVDTSKSRWRLEKEIGVRPETLMVFQRAQRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGGQDLVYKHGPARAKSLSWPAGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRKDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPRTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5822 - -


  Protein sequence: 1173 a.a.    

>T6CP015470 NZ_CH902589:1447784-1451302 [Vibrio alginolyticus 12G01] [TssM]
MRIKEIGRIFTQRWFLGLIGVAACSIFIWVVGPLIAIAGYEPLKSDFQRLVTILVLVLLWAIFNVTKQHKQKVKEDESIQ
TLLEVDSLSDKEAASEIDLMRERIEQAVKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLGDSLGSDSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASVMSQTRTERSLHAR
AIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAR
MLRILETEDDLDKRALIFEFPKQLRVLQANLDEFLAEIFAQNSFEEPALIRGVFMLSSVQEGLPVDKLMNESTNGLGLNR
LALAKSQTGSHSYFVKNLFDKVIFKEQLLGTVNRHYQKQSGWMRRGVYIGCTTLLLGASALWFMSYKWNSQLIVDTNEQV
SHIDAMIGAQSLDFEADIVSAVETLDRLMTLPLGKQSDYGDADSVKRFGLYQGDKVSQAANNAYSDALTQYYAPLLLDSL
VSEMESNQQHREYLYETLKTYLMLFNPDKYQQDEVLAWFNFYFERQYPGELNADLRRRLLQHTQYLLGNDGQGFTYNTAA
VTSAREVLTQMSLPERAYQRMKIQFSKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KSLMEENWVYGDDLKAHEIDQDSAIQGVQSRYYQDYVNEWKTLIEDIQLKQAPSLELATEQARVLSGVERPIESLLRAIQ
KEVALSKVSLSENQKAASEVAGKVAKVKFSNTADRLDMYLPEENGFNVALPGKEVESHFSEILRLGEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKELLPRPFNQWLGELSQESVKFAESGSKDHLNQLWVTQVVRPY
QRTIAGRYPFEPNASREVRLKDFKRFFGYGGTLDAFFQEYLEPFVDTSKSRWRLEKEIGVRPETLMVFQRAQRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGGQDLVYKHGPARAKSLSWPAGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRKDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPRTL*

  Nucleotide sequence: 3519 bp    

>T6CP015470 NZ_CH902589:1447784-1451302 [Vibrio alginolyticus 12G01] [TssM]
ATGCGTATTAAAGAAATTGGCAGAATATTTACTCAAAGATGGTTTCTCGGTCTTATAGGTGTCGCTGCTTGTTCTATTTT
CATTTGGGTTGTTGGTCCACTTATTGCGATAGCGGGATATGAGCCCCTAAAGAGTGACTTTCAACGGCTAGTCACTATTC
TAGTGTTGGTGTTGCTTTGGGCGATATTTAACGTTACCAAGCAGCATAAGCAAAAGGTGAAAGAAGACGAAAGTATTCAA
ACGTTGCTTGAGGTTGATAGCCTATCAGATAAAGAAGCGGCAAGTGAAATTGACTTAATGCGCGAGCGTATTGAGCAAGC
TGTCAAAGTAGTGACGAAAACACACAAAGGCAAGAGAAGCTTGTATGACTTACCGTGGTATGTGTTGATTGGCCCTCCTG
GAACGGGGAAAACAACCGTATTAAAGCAGTCGGGGTTAGAGTTTCCATTAGGAGACAGCCTTGGCTCTGACTCTATTGCC
GGTGTAGGTGGCACTCGCCACTGTGATTGGTGGTTTGCAAATAAAGCAGTTCTTATAGATACGGCTGGCCGGTATACGAC
ACAAGACAGTCAAGAAAAGGTCGACTCTAAAGCGTGGCATGGTTTCTTAGGATTGTTGAAAAAGTATAGAACGCAAAGAC
CAATTAATGGTGCGATTGTTACGATAAGTTTAGCCAGCGTGATGTCTCAGACTCGAACCGAGCGAAGTCTTCATGCTCGA
GCAATTAAGTCGAGACTTCAAGAACTGAAAAACCAGTTAGGCATGCAGTTTCCGATTTACGTATTGCTCACCAAAATGGA
TTTAGTTGCCGGTTTCAATGAGTTTTTTGCCGATTTAAGTAAGGAAGAGCGAGAGGAATTATTTGGTTTCATGTTCCCTC
GTGAAGTGGATGATGAAAGAGGCGTTATTTCTCTATTCAACAAAGAATTTCATGGCATGTTGGAGCGCCTTGATGCCCGT
ATGCTTAGAATACTTGAAACAGAGGACGATTTAGATAAACGCGCGCTTATCTTTGAATTTCCAAAGCAATTAAGAGTGCT
ACAAGCTAATCTTGACGAGTTTTTAGCTGAAATTTTTGCACAAAACAGTTTTGAAGAGCCTGCCTTGATTCGTGGTGTGT
TTATGTTGAGTTCGGTTCAAGAAGGCTTACCAGTCGATAAGCTGATGAATGAATCAACCAATGGACTTGGGTTAAATCGT
TTAGCTCTAGCAAAGAGTCAGACTGGTTCTCATAGTTATTTCGTAAAGAATCTTTTTGACAAGGTTATTTTCAAAGAGCA
ACTGTTAGGAACAGTTAATCGTCACTATCAAAAGCAGTCAGGTTGGATGCGTCGAGGTGTTTACATTGGATGTACAACGC
TACTGCTAGGAGCTTCTGCGCTTTGGTTTATGAGTTATAAGTGGAACTCACAGTTAATTGTCGATACCAATGAACAAGTG
AGCCATATTGATGCAATGATTGGCGCTCAAAGTTTAGATTTTGAAGCGGATATTGTTTCAGCTGTAGAAACATTAGATAG
GTTGATGACGCTTCCTCTCGGAAAGCAAAGCGACTACGGTGATGCCGACTCTGTAAAGAGGTTTGGTCTCTATCAAGGAG
ACAAAGTGTCTCAGGCGGCAAACAATGCATATTCTGATGCTTTGACCCAATATTACGCCCCTCTGCTTCTTGATAGCTTG
GTGTCAGAAATGGAGAGCAATCAACAGCATCGTGAGTATTTGTACGAAACGTTAAAAACGTATTTGATGTTATTCAATCC
AGACAAATATCAACAAGATGAAGTGTTGGCTTGGTTTAACTTTTATTTCGAACGTCAATATCCGGGTGAGCTAAACGCAG
ACTTAAGAAGACGTTTGCTACAGCACACTCAATATTTATTAGGCAACGATGGACAAGGCTTTACTTACAACACTGCTGCC
GTGACGAGTGCTCGAGAAGTGTTGACTCAAATGTCTCTACCTGAACGTGCTTATCAGCGAATGAAAATACAATTTTCTAA
GAGTCATGTACCTTCATTTCGCTTGACTGATGTATTGGGACCTAAAGGGCTTGAGCAGTTCGAGCGAGCTAGTGGAAAAC
CGCTATCACAAGGCATTTCGGGTTTCTACACGTACAATGGTTTTCATAGCATTTTTCAGATTCAGATCAACCGTACAGTA
AAAAGCCTAATGGAAGAGAACTGGGTTTATGGTGACGATCTTAAGGCTCATGAAATTGATCAAGATAGTGCCATCCAAGG
CGTTCAGTCTCGATACTATCAAGATTATGTTAATGAATGGAAAACGTTGATCGAAGATATTCAATTGAAGCAGGCTCCGA
GCCTAGAACTTGCGACAGAGCAAGCGAGGGTTTTATCTGGAGTTGAAAGACCAATCGAATCTTTGTTGAGAGCAATCCAA
AAAGAGGTTGCTCTATCAAAAGTATCATTGTCAGAAAATCAAAAAGCCGCTTCTGAAGTTGCTGGTAAAGTTGCCAAAGT
TAAGTTTTCAAATACGGCAGATAGACTAGACATGTATTTACCTGAAGAAAACGGCTTCAATGTTGCTCTGCCAGGTAAAG
AAGTCGAATCGCATTTTTCAGAGATCTTACGCCTTGGTGAGCAAGACTTTGATGATATTCAACAAGCAATGGTTAACTTG
CGTTCGTATTTAAGTGACTTATCAAGCTCTGGTAACAATCAGAAAATTGCCTACAAAAGTATTTTAGATGGAACCGTTAC
ACAAGATGTCGCAGCTTCCTTCGCTCGTGCTAAAGAACTTTTGCCGAGACCATTTAATCAATGGCTAGGTGAGCTTTCTC
AAGAGTCGGTTAAGTTTGCGGAGAGCGGTTCGAAGGATCATCTAAATCAACTTTGGGTAACACAGGTGGTCCGCCCTTAT
CAGCGGACTATTGCTGGCAGATATCCATTCGAGCCCAACGCGAGCAGAGAAGTTAGATTGAAAGACTTTAAGCGATTCTT
TGGTTATGGAGGTACGCTTGACGCATTCTTCCAAGAATACCTCGAACCGTTTGTGGATACTTCGAAAAGTCGTTGGCGAT
TAGAAAAGGAAATTGGAGTTCGTCCAGAAACACTAATGGTCTTTCAAAGAGCGCAACGTATCAGGCAGTCTTTCTTTGAA
TCTGATAATTCATTACGCGTAGAGTTTGGGATGAAGCCTGTTTATCTAGACCAGCACATTACTCGATTTGTCTTAGAGTT
GGGTGGACAAGATTTGGTTTATAAGCATGGACCAGCAAGAGCTAAATCGTTGAGTTGGCCTGCCGGTCAAGATCAGACTC
GAATTGTGTTCACACCACCTGAATCTAAACGCGAAATTGCGCATACCTATGAAGGTGAGTGGGGCATATTCAAGCTTTTG
GATCAATCACTTAAAGCAAGGCCAGAATCGCGTAAAGACAATATAGTTATGATTGATTTAAAGGGAAATAAGGTGCAGCT
AGAGCTTATTCCTTCTAGTGCCATTAATCCGTTTTGGTCAAATGAAATGGAGAGGTTCAGATGTCCACGAACCTTATAA