Detailed information of effector
Summary
| Effector ID | EFF01535  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (160 a.a.); Nucleotide sequence (480 bp) |
| Description | hcp |
| Reference | [1] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | V12G01_07583 (hcp2) |
| Coordinates (Strand) | 1464340..1464819 (+) |
| NCBI ID | 491518336 |
| RefSeq | NZ_CH902589 |
| Uniprot ID | A0A2I3CRH4_VIBAX, S5J041_VIBPH, Q87HD8_VIBPA, A0A0T7E196_9VIBR, C0LZR3_VIBAL, D0X0J5_VIBAL |
| KEGG ID | vag:N646_4398, vpf:M634_23055, vpa:VPA1027 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 1.2e-28 | 6..133 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-159 | MQSNTYLKYADIKGEATAEQYKDLITLLSVDWSVGREISSYTGTAMDREASATRLYDLTITKLQDRSSPDLFKEATIGKGKPAVFHITKQGEKVEEIMKIELTDAMISNYSVSIQDDRPVETITISYTEMMMTVTPTDDKNNTSAPLVYGYSGVKGQQM |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9833 | - | - |
Protein sequence: 160 a.a. .
>EFF01535 NZ_CH902589:1464340-1464819 (Unknown) [Vibrio alginolyticus 12G01]
MQSNTYLKYADIKGEATAEQYKDLITLLSVDWSVGREISSYTGTAMDREASATRLYDLTITKLQDRSSPDLFKEATIGKG
KPAVFHITKQGEKVEEIMKIELTDAMISNYSVSIQDDRPVETITISYTEMMMTVTPTDDKNNTSAPLVYGYSGVKGQQM*
MQSNTYLKYADIKGEATAEQYKDLITLLSVDWSVGREISSYTGTAMDREASATRLYDLTITKLQDRSSPDLFKEATIGKG
KPAVFHITKQGEKVEEIMKIELTDAMISNYSVSIQDDRPVETITISYTEMMMTVTPTDDKNNTSAPLVYGYSGVKGQQM*
Nucleotide sequence: 480 bp .
>EFF01535 NZ_CH902589:1464340-1464819 (Unknown) [Vibrio alginolyticus 12G01]
ATGCAGTCTAATACGTATCTTAAATACGCAGATATTAAGGGTGAGGCAACAGCCGAGCAATACAAAGATTTGATTACACT
ACTATCTGTAGATTGGAGTGTAGGTCGTGAGATCTCATCATACACTGGTACAGCGATGGACCGTGAAGCGAGCGCTACTC
GTCTATATGATTTGACTATCACTAAACTTCAAGACCGTTCTTCTCCTGACTTATTTAAAGAAGCGACGATTGGTAAAGGT
AAACCAGCGGTTTTCCATATTACTAAGCAGGGTGAGAAGGTTGAAGAGATCATGAAGATCGAACTGACTGATGCGATGAT
TTCTAACTACTCAGTTTCTATCCAAGACGATCGTCCAGTTGAAACTATCACAATTTCTTACACAGAAATGATGATGACAG
TGACTCCTACTGACGATAAGAACAACACTTCTGCTCCATTGGTATACGGCTACAGCGGTGTTAAAGGTCAACAAATGTAA
ATGCAGTCTAATACGTATCTTAAATACGCAGATATTAAGGGTGAGGCAACAGCCGAGCAATACAAAGATTTGATTACACT
ACTATCTGTAGATTGGAGTGTAGGTCGTGAGATCTCATCATACACTGGTACAGCGATGGACCGTGAAGCGAGCGCTACTC
GTCTATATGATTTGACTATCACTAAACTTCAAGACCGTTCTTCTCCTGACTTATTTAAAGAAGCGACGATTGGTAAAGGT
AAACCAGCGGTTTTCCATATTACTAAGCAGGGTGAGAAGGTTGAAGAGATCATGAAGATCGAACTGACTGATGCGATGAT
TTCTAACTACTCAGTTTCTATCCAAGACGATCGTCCAGTTGAAACTATCACAATTTCTTACACAGAAATGATGATGACAG
TGACTCCTACTGACGATAAGAACAACACTTCTGCTCCATTGGTATACGGCTACAGCGGTGTTAAAGGTCAACAAATGTAA