Detailed information of regulator
Summary
| Regulator ID | REG0280  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (145 a.a.); Nucleotide sequence (435 bp) |
| Description | Transcriptional regulator AsnC,identified to bind to the promoters of both aphA and luxR. |
| Reference |
External database links
| Locus tag (Gene) | V12G01_10813 (AsnC) |
| Coordinates (Strand) | 181050..181484 (-) |
| NCBI ID | 91191052 |
| GenBank | AAPS01000004 |
| Uniprot ID | UPI0000D52C59 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| V12G01-21538 (luxR) promoter | TGAGTGTAA TGGTGCATATGCACAAGGTCAA | |
| V12G01-14830 (aphA) promoter | CACAAGTTAACACTATTGATTATTATGTTAGTTTT |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MerR-DNA-bind | PF09278.13 | 9.2e-18 | 45..109 |
| MerR | PF00376.25 | 1.7e-13 | 4..40 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-144 | MEISVGEVAKRSGVKVSALHFYEQKGLISSWRNQGNQRRYHRSVLRRVAVIKAAQQVGLSLEEIKHALDELPKHQAPNKAQWEEMASHWHEQLEQKIQKLKALQNDLGGCIGCGCLSLETCALRNPEDVLGKDHSGSNLTLEEE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9960 | - | - |
Protein sequence of regulator: 145 a.a. .
>REG0280 AAPS01000004:c181484-181050 [Vibrio alginolyticus 12G01]
MEISVGEVAKRSGVKVSALHFYEQKGLISSWRNQGNQRRYHRSVLRRVAVIKAAQQVGLSLEEIKHALDELPKHQAPNKA
QWEEMASHWHEQLEQKIQKLKALQNDLGGCIGCGCLSLETCALRNPEDVLGKDHSGSNLTLEEE*
MEISVGEVAKRSGVKVSALHFYEQKGLISSWRNQGNQRRYHRSVLRRVAVIKAAQQVGLSLEEIKHALDELPKHQAPNKA
QWEEMASHWHEQLEQKIQKLKALQNDLGGCIGCGCLSLETCALRNPEDVLGKDHSGSNLTLEEE*
Nucleotide sequence of regulator: 435 bp .
>REG0280 AAPS01000004:c181484-181050 [Vibrio alginolyticus 12G01]
ATGGAAATCAGCGTAGGAGAAGTGGCAAAACGTTCTGGAGTGAAAGTATCTGCCCTACATTTTTACGAGCAAAAAGGGCT
GATTTCGAGTTGGAGAAATCAAGGTAATCAGCGTCGCTACCATCGCAGTGTGTTGCGCCGTGTTGCGGTGATCAAAGCAG
CGCAGCAAGTTGGGTTGTCGTTAGAAGAAATTAAACATGCGTTAGATGAACTGCCGAAACACCAAGCCCCTAACAAAGCG
CAATGGGAAGAAATGGCAAGCCATTGGCATGAACAGTTAGAACAAAAGATTCAAAAACTTAAAGCGTTGCAAAACGATTT
AGGCGGATGCATTGGCTGCGGGTGCTTGTCGTTAGAAACCTGTGCACTGCGAAACCCAGAAGATGTATTAGGGAAAGACC
ACTCGGGGTCGAACTTAACGTTAGAAGAAGAGTAG
ATGGAAATCAGCGTAGGAGAAGTGGCAAAACGTTCTGGAGTGAAAGTATCTGCCCTACATTTTTACGAGCAAAAAGGGCT
GATTTCGAGTTGGAGAAATCAAGGTAATCAGCGTCGCTACCATCGCAGTGTGTTGCGCCGTGTTGCGGTGATCAAAGCAG
CGCAGCAAGTTGGGTTGTCGTTAGAAGAAATTAAACATGCGTTAGATGAACTGCCGAAACACCAAGCCCCTAACAAAGCG
CAATGGGAAGAAATGGCAAGCCATTGGCATGAACAGTTAGAACAAAAGATTCAAAAACTTAAAGCGTTGCAAAACGATTT
AGGCGGATGCATTGGCTGCGGGTGCTTGTCGTTAGAAACCTGTGCACTGCGAAACCCAGAAGATGTATTAGGGAAAGACC
ACTCGGGGTCGAACTTAACGTTAGAAGAAGAGTAG