Detailed information of effector
Summary
| Effector ID | EFF01454  |
| Effector type | Tde |
| Cognate immunity protein | IMU00336 |
| Related T6SS (Type) | T6SS00939 (Type i5) |
| Strain | Vibrio alginolyticus 12G01 |
| Replicon | chromosome |
| Sequence | Protein sequence (240 a.a.); Nucleotide sequence (720 bp) |
| Description | V12G01_16922 possess predicted nuclease |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | V12G01_RS33945 |
| Coordinates (Strand) | 213122..213841 (+) |
| NCBI ID | 491519344 |
| RefSeq | NZ_CH902590 |
| Uniprot ID | UPI0000D544CE |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| AHH | PF14412.8 | 2.1e-11 | 117..208 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-239 | MEKVILGKSPVLKLPLRPKNPSGLEMAIYRFQVKAYKHYHKIKDLDKTSPKEVKEAEETVAHLNRERAKINAHAQLQEQLLADLEAYRDDNKDKSSPELGMENHHPTNRLVSNLYAVSEPQPSPNHAAHHIVMGNGRVRAMTDARLQMFMFGIGINDSMNGIWLPRSTAYKGHYTTPNAPTHSRIHGNNYQRWVGKLADIRHNESAFKSRLVRIKSQLKDGSYPPQILMKKDPTWNPPE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9933 | - | - |
Protein sequence: 240 a.a. .
>EFF01454 NZ_CH902590:213122-213841 Type VI DNase and RNase Effector (Tde) [Vibrio alginolyticus 12G01]
MEKVILGKSPVLKLPLRPKNPSGLEMAIYRFQVKAYKHYHKIKDLDKTSPKEVKEAEETVAHLNRERAKINAHAQLQEQL
LADLEAYRDDNKDKSSPELGMENHHPTNRLVSNLYAVSEPQPSPNHAAHHIVMGNGRVRAMTDARLQMFMFGIGINDSMN
GIWLPRSTAYKGHYTTPNAPTHSRIHGNNYQRWVGKLADIRHNESAFKSRLVRIKSQLKDGSYPPQILMKKDPTWNPPE*
MEKVILGKSPVLKLPLRPKNPSGLEMAIYRFQVKAYKHYHKIKDLDKTSPKEVKEAEETVAHLNRERAKINAHAQLQEQL
LADLEAYRDDNKDKSSPELGMENHHPTNRLVSNLYAVSEPQPSPNHAAHHIVMGNGRVRAMTDARLQMFMFGIGINDSMN
GIWLPRSTAYKGHYTTPNAPTHSRIHGNNYQRWVGKLADIRHNESAFKSRLVRIKSQLKDGSYPPQILMKKDPTWNPPE*
Nucleotide sequence: 720 bp .
>EFF01454 NZ_CH902590:213122-213841 Type VI DNase and RNase Effector (Tde) [Vibrio alginolyticus 12G01]
ATGGAAAAGGTAATTTTGGGCAAGTCTCCCGTTCTGAAGCTCCCTTTGAGGCCAAAGAACCCGTCAGGCTTGGAGATGGC
GATATATAGATTTCAGGTTAAAGCGTACAAGCATTACCACAAGATAAAGGATCTAGATAAAACCTCGCCTAAAGAGGTTA
AAGAAGCAGAGGAAACCGTTGCTCATTTGAATCGAGAGCGTGCCAAGATTAACGCGCATGCACAATTGCAAGAGCAGTTG
CTTGCAGATTTAGAAGCGTATCGAGATGATAACAAAGATAAATCTAGTCCCGAGCTGGGGATGGAAAATCACCATCCTAC
AAATCGCTTGGTAAGTAACTTGTATGCTGTTTCAGAGCCGCAACCGTCCCCAAATCATGCAGCACATCATATCGTTATGG
GTAATGGTCGAGTTAGGGCCATGACTGACGCTCGGTTGCAAATGTTCATGTTTGGGATAGGTATTAACGACTCAATGAAT
GGTATTTGGCTACCACGTTCGACCGCCTACAAGGGACATTATACTACGCCAAATGCACCAACGCATTCGCGCATACATGG
CAATAATTATCAGAGATGGGTTGGTAAATTGGCCGATATTCGTCACAATGAAAGCGCGTTTAAATCTAGACTTGTGAGAA
TCAAATCTCAGTTAAAGGATGGTTCTTACCCTCCCCAAATCCTTATGAAGAAAGACCCAACTTGGAACCCACCTGAATGA
ATGGAAAAGGTAATTTTGGGCAAGTCTCCCGTTCTGAAGCTCCCTTTGAGGCCAAAGAACCCGTCAGGCTTGGAGATGGC
GATATATAGATTTCAGGTTAAAGCGTACAAGCATTACCACAAGATAAAGGATCTAGATAAAACCTCGCCTAAAGAGGTTA
AAGAAGCAGAGGAAACCGTTGCTCATTTGAATCGAGAGCGTGCCAAGATTAACGCGCATGCACAATTGCAAGAGCAGTTG
CTTGCAGATTTAGAAGCGTATCGAGATGATAACAAAGATAAATCTAGTCCCGAGCTGGGGATGGAAAATCACCATCCTAC
AAATCGCTTGGTAAGTAACTTGTATGCTGTTTCAGAGCCGCAACCGTCCCCAAATCATGCAGCACATCATATCGTTATGG
GTAATGGTCGAGTTAGGGCCATGACTGACGCTCGGTTGCAAATGTTCATGTTTGGGATAGGTATTAACGACTCAATGAAT
GGTATTTGGCTACCACGTTCGACCGCCTACAAGGGACATTATACTACGCCAAATGCACCAACGCATTCGCGCATACATGG
CAATAATTATCAGAGATGGGTTGGTAAATTGGCCGATATTCGTCACAATGAAAGCGCGTTTAAATCTAGACTTGTGAGAA
TCAAATCTCAGTTAAAGGATGGTTCTTACCCTCCCCAAATCCTTATGAAGAAAGACCCAACTTGGAACCCACCTGAATGA