Detailed information of regulator


Summary

Regulator ID REG0279 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00939 experimental (Type i5)
Strain Vibrio alginolyticus 12G01
Replicon chromosome
Sequence Protein sequence (293 a.a.); Nucleotide sequence (879 bp)
Description EMSAs and DNase I footprinting assay on both strands of PvqsA confirmed vqsA Bindind Site(VBS).; EMSAs and DNase I footprinting assay on both strands of PvqsA confirmed vqsA Bindind Site(VBS) and were indicated by red.
Reference
External database links

Locus tag (Gene) V12G01_18672 (vqsA)
Coordinates (Strand) 114208..115086 (+)
NCBI ID 91191289
GenBank AAPS01000003
Uniprot ID UPI0000D52E86
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
V12G01-21538 (luxR) promoter ↓ TGAGTGTAA TGGTGCATATGCACAAGGTCAA
V12G01-14830 (aphA) promoter ↓ CACAAGTTAACACTATTGATTATTATGTTAGTTTT
V12G01-18672 (vqsA) promotor ↓ TAACCTCATTTGATTGATAAACTCTGCTTTAGTTATTTCTTTATCAACTCTGGGTGTGTA
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
LysR_substrate PF03466.22 2.5e-35 89..289
HTH_1 PF00126.29 5.3e-15 5..63
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-292 MDKLTSMKVFAYVVEHGSFRNAAHHFDISPTMVGKHVSFLEQSLGTQLIHRTTRKQSLTEAGRLYYQECHRIIEDITNAENLIQTLINRPTGTVKVNCPVTYGKKVIAPIVADFLAEFPDLNVELMLDNNLIDPYRSEADVIIRIGELVDSSLVARKLGEYHMTYCASPSYLEQHAPIQSLDDLAHHHCLGFQYHQGESQQVVNMPSNTFGNSNTRLTSNNGDVLKFAALKSSGVLMQPKILVEQEIQSGALVEILQQHAPKPKPIHLLYRSKQLSLKNRTLVDFILSHVNP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9862 - -


  Protein sequence of regulator: 293 a.a.    .

>REG0279 AAPS01000003:114208-115086 [Vibrio alginolyticus 12G01]
MDKLTSMKVFAYVVEHGSFRNAAHHFDISPTMVGKHVSFLEQSLGTQLIHRTTRKQSLTEAGRLYYQECHRIIEDITNAE
NLIQTLINRPTGTVKVNCPVTYGKKVIAPIVADFLAEFPDLNVELMLDNNLIDPYRSEADVIIRIGELVDSSLVARKLGE
YHMTYCASPSYLEQHAPIQSLDDLAHHHCLGFQYHQGESQQVVNMPSNTFGNSNTRLTSNNGDVLKFAALKSSGVLMQPK
ILVEQEIQSGALVEILQQHAPKPKPIHLLYRSKQLSLKNRTLVDFILSHVNP*

  Nucleotide sequence of regulator: 879 bp    .

>REG0279 AAPS01000003:114208-115086 [Vibrio alginolyticus 12G01]
ATGGATAAATTAACAAGCATGAAGGTTTTCGCTTACGTGGTCGAACACGGCAGCTTTCGTAATGCAGCACACCACTTTGA
TATTTCACCGACCATGGTTGGCAAGCACGTGAGCTTTCTTGAGCAAAGCTTAGGTACACAGCTAATTCATCGCACCACGC
GCAAACAGTCCCTGACCGAAGCTGGCAGACTCTACTATCAAGAGTGCCACCGCATTATTGAAGACATCACCAACGCAGAG
AACCTGATCCAGACGCTGATCAACCGTCCAACCGGAACTGTAAAAGTTAACTGCCCTGTCACGTATGGCAAAAAGGTCAT
TGCTCCTATCGTGGCTGACTTCTTAGCTGAATTTCCCGATTTGAACGTTGAGCTAATGTTGGACAACAATTTGATTGACC
CATATCGAAGCGAAGCTGACGTGATTATTCGAATCGGTGAGTTGGTCGATTCGTCGTTAGTCGCGAGAAAATTGGGTGAA
TACCACATGACTTACTGCGCCTCACCCTCTTACCTTGAGCAACATGCCCCTATCCAATCACTTGATGACTTAGCACACCA
TCATTGTTTGGGGTTTCAGTATCACCAAGGAGAAAGCCAACAAGTGGTGAACATGCCATCTAATACCTTTGGAAACAGCA
ACACCCGATTAACGTCTAACAATGGCGATGTACTTAAGTTCGCCGCGTTAAAAAGCTCTGGTGTTCTTATGCAACCAAAG
ATCTTAGTTGAGCAGGAAATCCAATCTGGTGCCTTGGTAGAAATTCTCCAGCAACATGCTCCCAAGCCAAAACCGATCCA
CCTGCTTTACCGTTCAAAACAGCTTTCCCTTAAAAACCGTACCTTGGTCGATTTTATTCTTTCTCATGTGAATCCATAA