SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0275
Regulator type	protein
Regulatory pathway (from literatures)	QS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111)
Related T6SS	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (205 a.a.); Nucleotide sequence (615 bp)
Description	TfoX and LuxR co-regulate the T6SS2. EMSAs and DNase I footprinting assay on both strands of PvqsA confirmed LuxR Bindind Site(RBS).
Reference	[1] Metzger LC, Matthey N, Stoudmann C, et al. Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species. Environ Microbiol. 2019 Jul;21(7):2231-2247. doi: 10.1111/1462-2920.14562. Epub 2019 Mar 12. PMID: 30761714 [2] Gao X, Wang X, Mao Q, et al. VqsA, a Novel LysR-Type Transcriptional Regulator, Coordinates Quorum Sensing (QS) and Is Controlled by QS To Regulate Virulence in the Pathogen Vibrio alginolyticus. Appl Environ Microbiol. 2018 May 31;84(12). pii: e00444-18. doi: 10.1128/AEM.00444-18. Print 2018 Jun 15. PMID: 29625990

External database links

Locus tag (Gene)	V12G01_21538 (LuxR)
Coordinates (Strand)	13551..14165 (-)
NCBI ID	91187814
GenBank	AAPS01000053
Uniprot ID	UPI0000D533F3
KEGG ID	-
KEGG pathway (determined by BlastKOALA)	map02020; map02024; map05111
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS2 transcripts	↓	-
V12G01-18672 (vqsA) promotor		TAGAATCGTAGGTCGCATTCGCTTGCAA EMSAs and DNase I footprinting assay on both strands of PvqsA confirmed LuxR Bindind Site(RBS)

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
TetR_N	PF00440.25	1.8e-09	22..67

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-204	MDSIAKRPRTRLSPIKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDEVLNHVVRQFSNFLSDNIDLDLHARENIANITNAMIELVSQDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLVQNMFIKAIERGEVCDQHDSEHLANLFHGICYSLFVQANRAKGEAELKHLVNSYLDMLCIYNREH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9921	-	-

Protein sequence of regulator: 205 a.a. .

                                    >REG0275 AAPS01000053:c14165-13551 [Vibrio alginolyticus 12G01]
MDSIAKRPRTRLSPIKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDEVLNHVVRQFSNFLS
DNIDLDLHARENIANITNAMIELVSQDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLVQNMFIKAIERGEVCDQHDSE
HLANLFHGICYSLFVQANRAKGEAELKHLVNSYLDMLCIYNREH*

                                 

Nucleotide sequence of regulator: 615 bp .

                                    >REG0275 AAPS01000053:c14165-13551 [Vibrio alginolyticus 12G01]
ATGGACTCAATTGCAAAGAGACCTAGAACTAGGCTTTCTCCAATTAAGCGTAAGCAACAGTTAATGGAAATCGCATTGGA
AGTATTTGCGCGCCGCGGCATCGGTCGTGGTGGTCACGCAGATATCGCAGAAATTGCTCAAGTTTCTGTAGCGACTGTTT
TTAACTACTTCCCTACACGCGAAGACTTGGTGGATGAAGTTCTCAATCACGTCGTACGTCAGTTCTCAAACTTCCTATCG
GATAACATAGACCTAGACTTACATGCACGTGAAAACATCGCCAATATTACTAATGCGATGATCGAGCTTGTCAGCCAAGA
TTGTCACTGGCTAAAAGTATGGTTCGAGTGGAGTGCTTCAACACGTGACGAAGTATGGCCATTGTTTGTTTCAACTAACC
GTACTAACCAGTTGCTGGTACAAAACATGTTCATTAAAGCAATTGAACGTGGCGAAGTGTGTGACCAGCACGATTCAGAA
CACTTGGCAAACCTGTTCCACGGTATTTGTTACTCACTGTTTGTCCAAGCGAACCGCGCTAAAGGTGAAGCGGAGCTGAA
ACATCTAGTGAATTCATACTTAGATATGCTTTGCATTTACAACCGTGAACACTAA

                                