SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00339
Cognate effector	EFF01457
Related T6SS (Type)	T6SS00939 (Type i5)
Strain	Vibrio alginolyticus 12G01
Replicon	chromosome
Sequence	Protein sequence (315 a.a.); Nucleotide sequence (945 bp)
Description	-
Reference	[1] Salomon D, Klimko JA, Trudgian DC, et al. Type VI Secretion System Toxins Horizontally Shared between Marine Bacteria. PLoS Pathog. 2015 Aug 25;11(8):e1005128. doi: 10.1371/journal.ppat.1005128. eCollection 2015 Aug. PMID: 26305100

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	V12G01_RS33210
Coordinates (Strand)	50304..51248 (-)
NCBI ID	91188895
RefSeq	NZ_CH902590
Uniprot ID	UPI0000D52921
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-41	MAYLPFYITPEEFSAYQEEQEQKILNGMNLTVWHKHDIEEP
Transmembrane helix	42-64	FRYLYLSIAIAAVSPLFFLTYFL
Inside	65-70	HDSSEM
Transmembrane helix	71-93	ILTYILSGLTIIMSGVSYLTFGL
Outside	94-123	DNRYDYTFSDRGLVIKKRRNLPNWVNSAAQ
Transmembrane helix	124-157	VMGWIGAIVCVVMVGVAGPMVLVGAGGFILVSFG
Inside	158-314	MLKRQPDEPTEVRVGEREDWLFADYNKKRKVIKFFFKHDTCEYINIEHTKISRLHNRFHYYVFFKTVSDLESMVNQLATEYKLDCTEVADHKKLFEAKPEARLFGTPVCYKEYQTDEVFDLRASNAPLPEREYLYNGKWQTESEIEQSKSELTAAKV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9976	-	-

Protein sequence: 315 a.a. .

                                    >IMU00339 NZ_CH902590:c51248-50304 [Vibrio alginolyticus 12G01]
MAYLPFYITPEEFSAYQEEQEQKILNGMNLTVWHKHDIEEPFRYLYLSIAIAAVSPLFFLTYFLHDSSEMILTYILSGLT
IIMSGVSYLTFGLDNRYDYTFSDRGLVIKKRRNLPNWVNSAAQVMGWIGAIVCVVMVGVAGPMVLVGAGGFILVSFGMLK
RQPDEPTEVRVGEREDWLFADYNKKRKVIKFFFKHDTCEYINIEHTKISRLHNRFHYYVFFKTVSDLESMVNQLATEYKL
DCTEVADHKKLFEAKPEARLFGTPVCYKEYQTDEVFDLRASNAPLPEREYLYNGKWQTESEIEQSKSELTAAKV*

                                

Nucleotide sequence: 945 bp .

                                    >IMU00339 NZ_CH902590:c51248-50304 [Vibrio alginolyticus 12G01]
TTGGCTTATTTACCTTTCTATATTACCCCAGAAGAATTTTCAGCATACCAAGAAGAGCAGGAGCAAAAAATTCTTAACGG
GATGAATCTGACAGTCTGGCATAAGCACGATATTGAAGAGCCTTTTCGATACTTATACCTATCAATTGCTATAGCTGCTG
TCAGTCCATTATTTTTCCTTACCTATTTCCTACATGATAGTAGTGAAATGATCTTAACTTACATTTTATCGGGGCTGACG
ATAATCATGTCTGGTGTGTCTTATCTGACATTTGGTTTGGACAATCGGTATGATTACACCTTCTCCGATCGCGGGTTAGT
CATAAAAAAACGACGTAATTTACCGAATTGGGTAAACTCCGCGGCTCAAGTCATGGGCTGGATTGGAGCAATAGTTTGTG
TCGTGATGGTTGGAGTTGCTGGACCGATGGTTCTTGTCGGAGCCGGTGGCTTTATATTGGTTTCATTTGGTATGCTTAAA
CGTCAGCCTGATGAACCAACAGAAGTGAGGGTCGGAGAACGTGAAGACTGGCTCTTTGCTGATTACAATAAGAAAAGAAA
AGTAATTAAGTTCTTTTTTAAGCATGACACATGCGAATATATTAATATAGAACATACTAAGATTTCACGCTTACACAACC
GATTCCACTATTACGTATTTTTCAAAACCGTGTCTGACTTAGAGTCTATGGTCAATCAGCTAGCCACGGAATATAAGCTT
GACTGTACAGAAGTCGCCGACCACAAAAAGCTATTTGAAGCTAAGCCAGAGGCTAGACTGTTTGGTACGCCAGTTTGTTA
TAAAGAATATCAAACCGATGAGGTATTTGATTTAAGGGCGTCAAATGCCCCGCTCCCAGAGCGAGAGTACCTATATAACG
GCAAGTGGCAAACCGAGTCTGAAATCGAACAATCAAAATCTGAACTAACGGCAGCCAAGGTATAA

                                