Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS01276 experimental
T6SS type Type i4b
Strain Burkholderia cenocepacia strain K56-2
Replicon (RefSeq) chromosome (NZ_LAUA01000004)
Location 63074..82939
Function anti-eukaryotic
Comment T6SS plays an important role in the persistence and intracellular survival
References
[1] Aubert DF et al (2015) Quantification of Type VI secretion system activity in macrophages infected with Burkholderia cenocepacia. Microbiology. doi: 10.1099/mic.0.000174. [PudMed:26364149]
[2] Aubert DF et al (2008) A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. Infect Immun. 76(5):1979-91. [PudMed:18316384]
[3] Aubert DF et al (2013) The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 15(2):372-85. [PudMed:22830644]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTagFTssATssBTssCTssDTssETssFTssGTssHTssJTssKTssLTssM
Number1111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
WQ49_RS11755 58391..59368 + 978 WP_012492312.1 zinc-binding alcohol dehydrogenase family protein
WQ49_RS36095 59393..59545 + 153 WP_006481917.1 DUF1427 family protein
WQ49_RS11760 59573..60601 - 1029 WP_006481924.1 transporter
WQ49_RS11765 60813..61805 - 993 WP_012492311.1 hypothetical protein
WQ49_RS11770 61927..62772 + 846 WP_006488977.1 M15 family metallopeptidase
T6CP015858 TssM WQ49_RS11775 (tssM) 63074..67018 + 3945 WP_012492310.1 type VI secretion system membrane subunit TssM
T6CP015859 TagF WQ49_RS11780 (tagF) 67015..68004 + 990 WP_006483243.1 type VI secretion system-associated protein TagF
WQ49_RS11785 68009..68959 + 951 WP_006494417.1 OmpA family protein
T6CP015860 TssA WQ49_RS11790 (tssA) 69053..70174 - 1122 WP_006484398.1 type VI secretion system protein TssA
T6CP015861 TssH WQ49_RS11795 (tssH) 70219..72888 - 2670 WP_006484392.1 type VI secretion system ATPase TssH
T6CP015862 TssG WQ49_RS11800 (tssG) 72930..74030 - 1101 WP_006484394.1 type VI secretion system baseplate subunit TssG
T6CP015863 TssF WQ49_RS11805 (tssF) 73994..75829 - 1836 WP_006484396.1 type VI secretion system baseplate subunit TssF
T6CP015864 TssE WQ49_RS11810 (tssE) 75906..76391 - 486 WP_006477092.1 type VI secretion system baseplate subunit TssE
T6CP015865 TssD WQ49_RS11815 76454..76957 - 504 WP_006477093.1 type VI secretion system tube protein Hcp
T6CP015866 TssC WQ49_RS11820 (tssC) 77028..78518 - 1491 WP_006484397.1 type VI secretion system contractile sheath large subunit
T6CP015867 TssB WQ49_RS11825 (tssB) 78534..79049 - 516 WP_006484395.1 type VI secretion system contractile sheath small subunit
WQ49_RS11830 79096..79722 - 627 WP_012492309.1 hypothetical protein
T6CP015868 TssJ WQ49_RS11835 (tssJ) 80109..80708 + 600 WP_006485335.1 type VI secretion system lipoprotein TssJ
T6CP015869 TssK WQ49_RS11840 (tssK) 80814..82160 + 1347 WP_006477098.1 type VI secretion system baseplate subunit TssK
T6CP015870 TssL WQ49_RS11845 82157..82939 + 783 WP_006491869.1 type IVB secretion system protein IcmH/DotU
WQ49_RS11850 83053..83388 - 336 WP_006485334.1 hypothetical protein
WQ49_RS11855 83470..83787 - 318 WP_006485338.1 hypothetical protein
WQ49_RS11860 84069..84869 + 801 WP_006485337.1 transporter substrate-binding domain-containing protein
WQ49_RS11865 84979..85182 - 204 WP_006485326.1 hypothetical protein
WQ49_RS11875 85631..86152 - 522 WP_006485328.1 ClpXP protease specificity-enhancing factor
WQ49_RS11880 86224..86835 - 612 WP_006400565.1 glutathione S-transferase N-terminal domain-containing protein
WQ49_RS11885 86930..87688 - 759 WP_006477108.1 cytochrome c1
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0324 experimental WQ49_05050 (cepR) protein QS regulatory pathway Burkholderia cenocepacia strain K56-2 chromosome (LAUA01000002) 545988..546707 (-) 815852483 T6SS promotor (P (BCAL0340) ) (↓)
REG0325 experimental WQ49_20245 (cciR) protein QS regulatory pathway Burkholderia cenocepacia strain K56-2 chromosome (LAUA01000016) 843677..844378 (+) 815850423 Unknown (↑)
REG0326 experimental WQ49_19595 (atsR) protein TCS-RR Burkholderia cenocepacia strain K56-2 chromosome (LAUA01000016) 710895..712715 (+) 815849700 T6SS transcripts (↑); T6SS expression (↑)
REG0327 experimental WQ49_05060 (cepI) protein QS regulatory pathway Burkholderia cenocepacia strain K56-2 chromosome (LAUA01000002) 547435..548043 (+) 815852485 T6SS expression (↓)
REG0328 experimental WQ49_20250 (cciI) protein QS regulatory pathway Burkholderia cenocepacia strain K56-2 chromosome (LAUA01000016) 844665..845255 (+) 815850424 T6SS expression (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01849 experimental WQ49_11820 (hcp) - - Burkholderia cenocepacia strain K56-2 scf7180000000051 chromosome (NZ_LAUA01000004) 76454..76957 (-) 493523014

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector