Detailed information of regulator


Summary

Regulator ID REG0327 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS01276 experimental (Type i4b)
Strain Burkholderia cenocepacia strain K56-2
Replicon chromosome
Sequence Protein sequence (203 a.a.); Nucleotide sequence (609 bp)
Description T6SS activity was recorded as the ability of the bacteria to induce the formation of characteristic ectopic actin nucleations or ‘pearls’ around infected ANA‐1 macrophages. While few pearls were detected around macrophages infected with WT, no pearls were visible around macrophages infected with ΔcepI and ΔcepIΔcciI thus confirming that T6SS expression is QS regulated.
Reference
External database links

Locus tag (Gene) WQ49_05060 (cepI)
Coordinates (Strand) 547435..548043 (+)
NCBI ID 815852485
GenBank LAUA01000002
Uniprot ID A0A142PB84_9BURK
KEGG ID -
KEGG pathway (determined by BlastKOALA) map01100; map00270; map02020; map02024; map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS expression ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Autoind_synth PF00765.19 2.3e-22 17..186
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-202 MQTFVHEEGRLPHELAADLGRYRRRVFVEQLGWALPSANESFERDQFDRDDTVYVFARNADGDMCGCARLLPTTRPYLLKSLFADLVAEDMPLPQSAAVWELSRFAATDDEGGPGNAEWAVRPMLAAVVECAAQLGARQLIGVTFASMERLFRRIGIHAHRAGPPKQVDGRLVVACWIDIDPQTFAALGIEPGQAARQAIAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9809 - -


  Protein sequence of regulator: 203 a.a.    .

>REG0327 LAUA01000002:547435-548043 [Burkholderia cenocepacia]
MQTFVHEEGRLPHELAADLGRYRRRVFVEQLGWALPSANESFERDQFDRDDTVYVFARNADGDMCGCARLLPTTRPYLLK
SLFADLVAEDMPLPQSAAVWELSRFAATDDEGGPGNAEWAVRPMLAAVVECAAQLGARQLIGVTFASMERLFRRIGIHAH
RAGPPKQVDGRLVVACWIDIDPQTFAALGIEPGQAARQAIAA*

  Nucleotide sequence of regulator: 609 bp    .

>REG0327 LAUA01000002:547435-548043 [Burkholderia cenocepacia]
ATGCAGACCTTCGTTCACGAGGAAGGGCGGTTGCCACACGAACTCGCGGCGGATCTCGGGCGCTATCGGCGCCGCGTGTT
CGTCGAGCAGCTCGGTTGGGCGCTCCCGTCGGCGAACGAAAGTTTCGAGCGTGACCAGTTCGATCGCGACGATACCGTCT
ACGTGTTCGCGCGAAACGCCGACGGCGACATGTGCGGATGTGCGCGCCTGCTGCCGACGACGCGCCCGTATCTGCTGAAG
TCGCTGTTCGCCGACCTGGTCGCCGAGGACATGCCGCTGCCGCAATCGGCCGCCGTCTGGGAATTGTCGCGATTCGCGGC
GACCGACGACGAAGGCGGTCCGGGCAACGCCGAATGGGCCGTGCGGCCGATGCTCGCGGCCGTCGTCGAATGCGCGGCCC
AGCTCGGGGCGCGGCAGTTGATCGGCGTGACGTTCGCGAGCATGGAGCGGCTGTTCCGCCGGATCGGCATACACGCGCAC
CGCGCAGGCCCGCCGAAGCAGGTAGATGGGCGTCTGGTGGTCGCGTGCTGGATCGACATCGATCCGCAAACGTTTGCTGC
GCTAGGAATCGAGCCGGGGCAAGCCGCCCGGCAAGCTATCGCCGCCTGA