SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01849
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS01276 (Type i4b)
Strain	Burkholderia cenocepacia strain K56-2 scf7180000000051
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Description	hcp
Reference	[1] Spiewak HL, Shastri S, Zhang L, et al. Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition. Microbiologyopen. 2019 Jan 9:e774. doi: 10.1002/mbo3.774. [Epub ahead of print] PMID: 30628184 [2] Aubert DF, Hu S, Valvano MA. Quantification of type VI secretion system activity in macrophages infected with Burkholderia cenocepacia. Microbiology (Reading). 2015 Nov;161(11):2161-73. doi: 10.1099/mic.0.000174. Epub 2015 Sep 11. PMID: 26364149

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	WQ49_11820 (hcp)
Coordinates (Strand)	76454..76957 (-)
NCBI ID	493523014
RefSeq	NZ_LAUA01000004
Uniprot ID	J7JDR9_BURCE, A0A0B1YQS5_9BURK, A0A0U4DCL3_BURCE, A0A084D9C9_9BURK, A0A2S9KZ71_9BURK, A0A1K1S9E0_9BURK, A0A117VGM3_9BURK, A0A1B4F605_9BURK, A0A103LBA0_9BURK, A0A2A4CEA2_9BURK, A0A1X1P7H9_9BURK, A0A2A9LW14_9BURK, A0A228PYE3_9BURK, A0A228HBG8_9BURK, A0A228I0N3_9BURK, A0A228J4P0_9BURK, A0A228K684_9BURK, A0A228RK00_9BURK, A0A228QKE8_9BURK, B4E6R5_BURCJ, A0A2U9SGD4_9BURK, A0A081V0J2_BURCE, A0A0H2XRS0_BURCA, Q0BIT1_BURCM, B1FDD0_9BURK, B1T015_9BURK, B1YS99_BURA4, B1JUN9_BURCC, Q39K54_BURL3, A0A0G3YXS0_9BURK, A0A1B4HQA6_9BURK, A0A1B4BP34_9BURK, A0A087NNS3_BURPY, A0A1D7ZEA5_9BURK, A0A0M1IMJ7_9BURK
KEGG ID	bdf:WI26_01920, bstl:BBJ41_12130, bur:Bcep18194_A3561, bam:Bamb_0383, bced:DM42_1364, bcon:NL30_20125, bcj:BCAL0343, bct:GEM_3042, bpyr:ABD05_07980, bcn:Bcen_2637, bmec:WJ16_01965, bac:BamMC406_0404, bcm:Bcenmc03_0447
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	3.1e-37	10..138

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-167	MLHMHLQFGSPAVKGESADKDHQGWIELKSWDHSIVQPRSATASTAGGHTMTRCEHGDMIFTKEIDSSSPLLYQHASGGTTFDEVTVHFSRADGEGKRVQYLEVKLKYVIISSIAPSVREEGLPLETFSLKYAAVQWKQTQQKIGGNQGGNTQGAWSLTKNDKTYAV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9447	-	-

Protein sequence: 168 a.a. .

                                    >EFF01849 NZ_LAUA01000004:c76957-76454  (Unknown) [Burkholderia cenocepacia]
MLHMHLQFGSPAVKGESADKDHQGWIELKSWDHSIVQPRSATASTAGGHTMTRCEHGDMIFTKEIDSSSPLLYQHASGGT
TFDEVTVHFSRADGEGKRVQYLEVKLKYVIISSIAPSVREEGLPLETFSLKYAAVQWKQTQQKIGGNQGGNTQGAWSLTK
NDKTYAV*

                                

Nucleotide sequence: 504 bp .

                                    >EFF01849 NZ_LAUA01000004:c76957-76454  (Unknown) [Burkholderia cenocepacia]
ATGTTACATATGCACTTGCAGTTTGGTAGTCCCGCGGTGAAGGGCGAATCCGCGGACAAGGACCATCAGGGCTGGATCGA
ACTGAAATCGTGGGATCACTCGATCGTTCAGCCGCGTTCGGCAACCGCATCGACCGCTGGCGGTCACACGATGACGCGTT
GCGAGCACGGCGACATGATCTTCACGAAGGAAATCGATTCGTCGAGCCCGCTGCTGTACCAGCACGCTTCGGGCGGTACC
ACGTTCGACGAAGTGACGGTCCATTTCTCGCGCGCGGACGGTGAAGGCAAGCGCGTGCAGTATCTGGAAGTCAAGCTCAA
ATACGTGATCATCTCGAGCATCGCCCCGAGCGTCCGCGAGGAAGGTCTGCCCCTCGAGACGTTCTCGCTGAAGTACGCAG
CCGTGCAGTGGAAGCAAACCCAGCAGAAGATCGGCGGCAACCAGGGCGGCAACACGCAAGGCGCCTGGAGCCTGACGAAG
AACGACAAGACCTACGCGGTCTAA

                                