Detailed information of regulator


Summary

Regulator ID REG0324 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Quorum sensing (map02024)
Related T6SS T6SS01276 experimental (Type i4b)
Strain Burkholderia cenocepacia strain K56-2
Replicon chromosome
Sequence Protein sequence (240 a.a.); Nucleotide sequence (720 bp)
Description Transcriptional profiling using a custom B. cenocepacia oligonucleotide microarray results that CepR was determined to positively regulate T6SS gene expression
Reference
External database links

Locus tag (Gene) WQ49_05050 (cepR)
Coordinates (Strand) 545988..546707 (-)
NCBI ID 815852483
GenBank LAUA01000002
Uniprot ID UPI00017BA09B
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020; map02024
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS promotor (P (BCAL0340) ) ↓ CAGTAAAACTTGCGCATT Identification of potential CepR regulated genes in T6SS(BCAL0340) promotor
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Autoind_bind PF03472.17 2.5e-32 18..157
GerE PF00196.21 5.6e-21 175..230
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-239 MELRWQDAYQQFSAAEDEQQLFQRIAAYSKRLGFEYCCYGIRVPLPVSKPAVAIFDTYPDGWMAHYQAQNYIEIDSTVRDGALNTNMIVWPDVDRIDPCPLWQDARDFGLSVGVAQSSWAARGAFGLLSIARHADRLTPAEINMLTLQTNWLANLSHSLMSRFMVPKLSPAAGVTLTAREREVLCWTAEGKTACEIGQILSISERTVNFHVNNILEKLGATNKVQAVVKAISAGLIEAP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9749 - -


  Protein sequence of regulator: 240 a.a.    .

>REG0324 LAUA01000002:c546707-545988 [Burkholderia cenocepacia]
MELRWQDAYQQFSAAEDEQQLFQRIAAYSKRLGFEYCCYGIRVPLPVSKPAVAIFDTYPDGWMAHYQAQNYIEIDSTVRD
GALNTNMIVWPDVDRIDPCPLWQDARDFGLSVGVAQSSWAARGAFGLLSIARHADRLTPAEINMLTLQTNWLANLSHSLM
SRFMVPKLSPAAGVTLTAREREVLCWTAEGKTACEIGQILSISERTVNFHVNNILEKLGATNKVQAVVKAISAGLIEAP*

  Nucleotide sequence of regulator: 720 bp    .

>REG0324 LAUA01000002:c546707-545988 [Burkholderia cenocepacia]
ATGGAACTGCGCTGGCAGGATGCCTACCAACAATTCAGCGCCGCGGAGGACGAGCAGCAGCTCTTCCAGCGGATCGCCGC
CTATTCCAAGCGGCTCGGATTCGAATACTGCTGTTACGGCATTCGCGTACCGCTGCCCGTTTCGAAGCCGGCCGTCGCCA
TCTTCGATACCTATCCGGACGGCTGGATGGCGCACTACCAGGCGCAGAACTACATCGAGATCGATTCGACGGTTCGTGAC
GGCGCGCTCAACACCAACATGATCGTGTGGCCGGACGTCGACCGGATCGATCCGTGCCCGCTGTGGCAGGACGCGCGCGA
TTTCGGATTGTCGGTGGGCGTCGCGCAGTCGAGCTGGGCGGCGCGCGGCGCGTTCGGGCTGCTAAGCATCGCCCGCCATG
CCGACCGGCTGACGCCGGCCGAGATCAACATGCTGACGCTGCAGACCAACTGGCTCGCAAACCTGTCGCATTCGCTGATG
AGCCGCTTCATGGTGCCGAAGCTGTCGCCGGCGGCGGGCGTCACGCTGACCGCGCGCGAGCGCGAGGTGCTGTGCTGGAC
GGCCGAGGGCAAGACCGCGTGCGAAATCGGCCAGATCCTCAGCATCTCGGAGCGGACGGTCAACTTCCACGTGAACAACA
TCCTCGAGAAGCTCGGCGCGACCAACAAGGTCCAGGCGGTCGTCAAGGCGATCTCGGCCGGGCTCATCGAAGCACCCTGA