Detailed information of component protein


Summary

Component ID T6CP015868
Component protein type TssJ
T6SS ID (Type) T6SS01276 experimental (Type i4b)
Strain Burkholderia cenocepacia strain K56-2
Replicon chromosome
Sequence Protein sequence (200 a.a.); Nucleotide sequence (600 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) WQ49_RS11835
Coordinate (Strand) 80109..80708 (+)
NCBI ID WP_006485335.1
RefSeq NZ_LAUA01000004
Uniprot ID UPI00017B90FD
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 8.1e-30 50..168
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MIRY
Transmembrane helix 5-27 ALPLLACALLAGCAAAPLLGSAA
Outside 28-199 SAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALKDPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKSGIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.8408 pos: 16-17. LAG-CA. MIRYALPLLACALLAG


  Protein sequence: 200 a.a.    

>T6CP015868 NZ_LAUA01000004:80109-80708 [Burkholderia cenocepacia] [TssJ]
MIRYALPLLACALLAGCAAAPLLGSAASAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALK
DPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKS
GIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG*

  Nucleotide sequence: 600 bp    

>T6CP015868 NZ_LAUA01000004:80109-80708 [Burkholderia cenocepacia] [TssJ]
ATGATTCGCTACGCGCTGCCGCTGCTTGCGTGCGCCCTTCTGGCCGGATGCGCGGCCGCTCCGCTGCTCGGCTCGGCCGC
GAGCGCCGTGATGTCCGCGGCCGGCATCGGCAAGCCCGAGGTGCCCGACGCGCAGAAGCCGCCGCGCAACATAGGCCTCA
CGCTGGCCGCCGCACCGAACCTGAATGCCGCGACCGACAACAAGCCGCTTGCGCTCGTCGTGCGCCTCTATGCGCTGAAA
GACCCGACCTCGTTCCAGCAAGCGCCGTTCGACGCATTTACCGACCCGGCCAAGGAAAAAACCGCACTCGGCGCCGATCT
GCTGAACGTGCGTGAAATCACGCTGATTCCGGGCCAGCGCTATACGGCAACCGAGAAGGTATCGCGCGAAGCGCAGGCAT
TCGGCATTGTCGCGCTGTTCCGCGATCCCGCACTGCAACGCTGGAAACTGACGTTCGATCCGGTGAAGTCGGAGAAATCC
GGCATAATCATCGGCCTGCATAATTGCGCGATGACGGTCACCGACGGCACGGTCATCGCACCGCAACAGGGTGCGCCTTC
GCAACCGCTGAATATGCTGTCTTCGGTCTCCTGCGGTTAA