Detailed information of regulator


Summary

Regulator ID REG0326 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01276 experimental (Type i4b)
Strain Burkholderia cenocepacia strain K56-2
Replicon chromosome
Sequence Protein sequence (607 a.a.); Nucleotide sequence (1821 bp)
Description Mutation in atsR, a RetS homologue, increases T6SS-dependent growth inhibition of Dictyostelium discoideum by B. cenocepacia; T6SS activity was recorded as the ability of the bacteria to induce the formation of characteristic ectopic actin nucleations or ‘pearls’ around infected ANA‐1 macrophages. Numerous pearls, indicative of T6SS activity, were produced by macrophages infected with ΔatsR, ΔatsRΔcepI and ΔatsRΔcepIΔcciI. AtsR represses T6SS activity independently of AHL‐QS.
Reference
External database links

Locus tag (Gene) WQ49_19595 (atsR)
Coordinates (Strand) 710895..712715 (+)
NCBI ID 815849700
GenBank LAUA01000016
Uniprot ID UPI00017B9BCF
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts ↑ -
T6SS expression ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 1.9e-23 347..460
Response_reg PF00072.26 1.3e-12 489..600
HisKA PF00512.27 2.4e-10 236..298
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MTRRRWKNRKII
Transmembrane helix 13-35 LVLGSLWIMGFAAWAFLLWDLLA
Outside 36-181 TSVNEGVLEGPREGVFWTAAQYRNVYTRFDRQLILYATREDEDFDHLQMQLDSLSVSFGFLQRPSEVSQYWLRIPRARNEIDALGEFMTRLKREVPLLRESPMQARQVIDEVNAYWPKVNGLANYFRAIEMAQRDFTFHQLKDKQR
Transmembrane helix 182-204 AILILGIVLGVLLCALFLLLLYT
Inside 205-606 MRTRDDLLERQNAALDAERKASDRAFEMIEAKNAFLGMVSHELRTPLQAICGSIEILLARSQSDANLKTIRRLQNSALSLEALVKDLTDYIKLRSTKRVAELETVGIAPLLAEALEPLREKIVAKRIAVTQLVEPPGLAIRSDRKLLRQVLSNLIENSVKYTVGGSITVAITLADAAAGQQLKIAVRDTGAGIAKQHLSKIFEPFYRANDAVGLHVDGIGMGLAVVREIVTTLRGHVDVRSVVGEGSEFVVTLPVEVPGVDTAGTAAGDAPAPPPALAYHGRRALVVDDNDNARETLGAMLSALGVDADLCGTGQEGVARFGTGRYDLVVLDLELPDLSGFDVARRIRAVAEPDDNGRHPAILGVSAYESAALRENQRVFDAFLPKPVHLRELGALVETLLA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9758 - -


  Protein sequence of regulator: 607 a.a.    .

>REG0326 LAUA01000016:710895-712715 [Burkholderia cenocepacia]
MTRRRWKNRKIILVLGSLWIMGFAAWAFLLWDLLATSVNEGVLEGPREGVFWTAAQYRNVYTRFDRQLILYATREDEDFD
HLQMQLDSLSVSFGFLQRPSEVSQYWLRIPRARNEIDALGEFMTRLKREVPLLRESPMQARQVIDEVNAYWPKVNGLANY
FRAIEMAQRDFTFHQLKDKQRAILILGIVLGVLLCALFLLLLYTMRTRDDLLERQNAALDAERKASDRAFEMIEAKNAFL
GMVSHELRTPLQAICGSIEILLARSQSDANLKTIRRLQNSALSLEALVKDLTDYIKLRSTKRVAELETVGIAPLLAEALE
PLREKIVAKRIAVTQLVEPPGLAIRSDRKLLRQVLSNLIENSVKYTVGGSITVAITLADAAAGQQLKIAVRDTGAGIAKQ
HLSKIFEPFYRANDAVGLHVDGIGMGLAVVREIVTTLRGHVDVRSVVGEGSEFVVTLPVEVPGVDTAGTAAGDAPAPPPA
LAYHGRRALVVDDNDNARETLGAMLSALGVDADLCGTGQEGVARFGTGRYDLVVLDLELPDLSGFDVARRIRAVAEPDDN
GRHPAILGVSAYESAALRENQRVFDAFLPKPVHLRELGALVETLLA*

  Nucleotide sequence of regulator: 1821 bp    .

>REG0326 LAUA01000016:710895-712715 [Burkholderia cenocepacia]
ATGACGCGGCGGCGATGGAAGAATAGAAAAATCATCCTGGTCCTCGGCTCGCTGTGGATCATGGGGTTCGCCGCGTGGGC
GTTCCTGCTGTGGGATCTCCTCGCCACCTCGGTCAACGAGGGCGTGCTCGAGGGGCCGCGCGAAGGCGTGTTCTGGACCG
CCGCGCAATACCGCAACGTGTATACGCGCTTCGATCGTCAGCTGATTCTTTACGCGACGCGCGAGGACGAAGACTTCGAT
CACCTGCAGATGCAGCTCGACAGCCTGTCGGTGTCGTTCGGGTTCCTGCAGCGGCCGTCGGAAGTGTCCCAATACTGGCT
GCGCATCCCGCGCGCGCGCAACGAGATCGACGCGCTGGGCGAATTCATGACGCGCCTGAAGCGCGAGGTGCCGCTGCTGC
GCGAGTCGCCGATGCAGGCGCGGCAGGTGATCGACGAAGTCAATGCGTATTGGCCGAAAGTTAACGGCCTCGCGAACTAC
TTCCGCGCGATCGAAATGGCTCAGCGCGATTTCACGTTCCATCAACTGAAGGACAAACAGCGCGCGATCCTGATCCTCGG
CATCGTGCTCGGTGTGCTCCTGTGCGCGCTGTTCCTGCTGCTGCTCTATACGATGCGCACGCGCGACGACCTGCTCGAAC
GGCAAAACGCCGCGCTCGATGCGGAGCGCAAGGCGTCGGACCGCGCGTTCGAGATGATCGAGGCGAAGAATGCGTTCCTC
GGGATGGTCAGCCACGAACTGCGCACGCCGCTGCAGGCGATCTGCGGATCGATCGAGATCCTGCTCGCGCGCTCGCAGTC
CGACGCGAACCTGAAGACGATTCGGCGGCTGCAGAACTCCGCGCTGTCGCTGGAGGCGCTCGTCAAGGACCTCACCGACT
ACATCAAGCTGCGCTCGACGAAGCGCGTCGCCGAACTGGAGACCGTCGGCATCGCGCCGCTGCTCGCCGAAGCGCTCGAA
CCGCTACGCGAGAAGATCGTCGCGAAACGGATCGCGGTCACGCAGCTCGTCGAGCCGCCCGGCCTCGCGATCCGCTCCGA
TCGCAAGCTGCTGCGACAGGTGCTGTCGAATCTGATCGAGAACTCGGTCAAGTACACTGTCGGCGGCTCGATCACGGTCG
CGATCACGCTTGCCGACGCGGCGGCCGGCCAGCAATTGAAGATCGCCGTGCGCGACACCGGCGCCGGCATCGCGAAACAA
CATCTGTCGAAGATCTTCGAGCCGTTCTATCGCGCGAACGACGCGGTCGGGCTGCACGTCGACGGGATCGGCATGGGGCT
CGCGGTCGTGCGCGAAATCGTGACGACGCTACGCGGGCATGTGGACGTGCGCAGCGTGGTCGGCGAAGGCAGCGAGTTCG
TCGTGACGCTGCCGGTCGAAGTGCCGGGCGTCGATACGGCCGGCACTGCCGCCGGCGATGCGCCCGCGCCGCCGCCCGCG
CTGGCGTACCACGGCCGGCGCGCGCTGGTGGTCGACGACAACGACAACGCGCGCGAAACGCTCGGCGCGATGCTCTCGGC
GCTCGGCGTCGACGCCGATCTGTGCGGCACCGGGCAGGAAGGCGTCGCGCGCTTCGGCACGGGCCGCTACGACCTCGTCG
TGCTCGATCTCGAACTGCCGGACCTGAGCGGATTCGACGTGGCGCGGCGTATTCGGGCGGTGGCGGAACCCGACGACAAC
GGTCGGCATCCGGCGATCCTCGGCGTCAGCGCGTATGAATCGGCGGCGCTGCGCGAGAACCAGCGGGTGTTCGACGCGTT
CCTGCCGAAGCCGGTCCACCTGCGCGAGCTCGGCGCGCTCGTCGAGACACTGCTCGCCTGA