Detailed information of regulator
Summary
| Regulator ID | REG0328  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01276 (Type i4b) |
| Strain | Burkholderia cenocepacia strain K56-2 |
| Replicon | chromosome |
| Sequence | Protein sequence (197 a.a.); Nucleotide sequence (591 bp) |
| Description | T6SS activity was recorded as the ability of the bacteria to induce the formation of characteristic ectopic actin nucleations or ‘pearls’ around infected ANA‐1 macrophages. While few pearls were detected around macrophages infected with WT, no pearls were visible around macrophages infected with ΔcepI and ΔcepIΔcciI thus confirming that T6SS expression is QS regulated. |
| Reference |
External database links
| Locus tag (Gene) | WQ49_20250 (cciI) |
| Coordinates (Strand) | 844665..845255 (+) |
| NCBI ID | 815850424 |
| GenBank | LAUA01000016 |
| Uniprot ID | UPI0005BC1A53 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS expression | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Autoind_synth | PF00765.19 | 1.7e-15 | 12..171 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-110 | MPTTMHRRLGVFRYDVFVGRLGWQLPGADATSLTEWDQFDRGRTIHVVSVDQAQHICGCARLIPTTQPYLLQTLCAPSAALHLPRAPTVWELSRFAARCGANPTMRASTG |
| Transmembrane helix | 111-133 | MQLFPAILAIAASLGATCVIGAM |
| Inside | 134-196 | TRAVARLYQRCGLALQLLDTAETADRPAYLIGAIEITRSTFTNLGCDAHELLAAVTWLGAHAT |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8423 | - | - |
Protein sequence of regulator: 197 a.a. .
>REG0328 LAUA01000016:844665-845255 [Burkholderia cenocepacia]
MPTTMHRRLGVFRYDVFVGRLGWQLPGADATSLTEWDQFDRGRTIHVVSVDQAQHICGCARLIPTTQPYLLQTLCAPSAA
LHLPRAPTVWELSRFAARCGANPTMRASTGMQLFPAILAIAASLGATCVIGAMTRAVARLYQRCGLALQLLDTAETADRP
AYLIGAIEITRSTFTNLGCDAHELLAAVTWLGAHAT*
MPTTMHRRLGVFRYDVFVGRLGWQLPGADATSLTEWDQFDRGRTIHVVSVDQAQHICGCARLIPTTQPYLLQTLCAPSAA
LHLPRAPTVWELSRFAARCGANPTMRASTGMQLFPAILAIAASLGATCVIGAMTRAVARLYQRCGLALQLLDTAETADRP
AYLIGAIEITRSTFTNLGCDAHELLAAVTWLGAHAT*
Nucleotide sequence of regulator: 591 bp .
>REG0328 LAUA01000016:844665-845255 [Burkholderia cenocepacia]
ATGCCAACCACGATGCATCGACGCCTCGGCGTGTTCCGATACGACGTGTTTGTAGGTCGACTGGGTTGGCAGTTACCCGG
AGCGGACGCGACCAGCCTGACCGAATGGGATCAATTTGATCGCGGTCGTACGATTCACGTTGTCTCGGTCGATCAAGCTC
AGCACATTTGCGGGTGTGCACGATTGATACCGACCACACAGCCGTATTTGCTCCAGACACTGTGCGCGCCTTCAGCGGCG
CTGCACTTGCCACGCGCGCCCACGGTCTGGGAGCTGTCGCGGTTTGCCGCCCGCTGTGGGGCGAACCCGACCATGCGCGC
ATCGACTGGAATGCAGCTGTTTCCAGCGATTCTCGCAATCGCCGCGTCACTGGGCGCCACATGCGTCATCGGTGCCATGA
CGCGCGCCGTGGCACGGCTTTACCAGCGTTGCGGTCTGGCGCTCCAACTCCTCGACACTGCCGAAACCGCAGATCGTCCA
GCCTACCTGATCGGTGCAATCGAGATCACCCGATCGACGTTCACGAACCTGGGGTGCGACGCGCACGAGCTGTTGGCCGC
CGTGACATGGCTCGGCGCCCATGCCACGTGA
ATGCCAACCACGATGCATCGACGCCTCGGCGTGTTCCGATACGACGTGTTTGTAGGTCGACTGGGTTGGCAGTTACCCGG
AGCGGACGCGACCAGCCTGACCGAATGGGATCAATTTGATCGCGGTCGTACGATTCACGTTGTCTCGGTCGATCAAGCTC
AGCACATTTGCGGGTGTGCACGATTGATACCGACCACACAGCCGTATTTGCTCCAGACACTGTGCGCGCCTTCAGCGGCG
CTGCACTTGCCACGCGCGCCCACGGTCTGGGAGCTGTCGCGGTTTGCCGCCCGCTGTGGGGCGAACCCGACCATGCGCGC
ATCGACTGGAATGCAGCTGTTTCCAGCGATTCTCGCAATCGCCGCGTCACTGGGCGCCACATGCGTCATCGGTGCCATGA
CGCGCGCCGTGGCACGGCTTTACCAGCGTTGCGGTCTGGCGCTCCAACTCCTCGACACTGCCGAAACCGCAGATCGTCCA
GCCTACCTGATCGGTGCAATCGAGATCACCCGATCGACGTTCACGAACCTGGGGTGCGACGCGCACGAGCTGTTGGCCGC
CGTGACATGGCTCGGCGCCCATGCCACGTGA