SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0023
Regulator type	protein
Regulatory pathway (from literatures)	QS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111)
Related T6SS	T6SS01168 (Type i1)
Strain	Vibrio cholerae C6706
Replicon	chromosome
Sequence	Protein sequence (204 a.a.); Nucleotide sequence (612 bp)
Description	Hcp expression was dramatically decreased when hapR was disrupted in either the ΔluxO or ΔluxO tsrA strains.; T6SS-mediated killing are positively regulated by CytR, TfoX, HapR, and QstR.
Reference	[1] Zheng J, Shin OS, Cameron DE, et al. Quorum sensing and a global regulator TsrA control expression of type VI secretion and virulence in Vibrio cholerae. Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21128-33. doi: 10.1073/pnas.1014998107. Epub 2010 Nov 17. PMID: 21084635 [2] Watve SS, Thomas J, Hammer BK. CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae. PLoS One. 2015 Sep 24;10(9):e0138834. doi: 10.1371/journal.pone.0138834. eCollection 2015. PMID: 26401962

External database links

Locus tag (Gene)	GPY04_RS00870 (HapR)
Coordinates (Strand)	164935..165546 (-)
NCBI ID	446262243
RefSeq	NZ_CP046844
Uniprot ID	B2CKP3_VIBCL
KEGG ID	vcq:EN18_19905
KEGG pathway (determined by BlastKOALA)	map02020; map02024; map05111
PDB ID	6D7R

Target

Target	Regulation (↓/↑)	Target sequence
Unknown	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
TetR_N	PF00440.25	5.2e-09	23..67

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-203	MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFLTDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDVDNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9940	-	-

Protein sequence of regulator: 204 a.a. .

                                    >REG0023 NZ_CP046844:c165546-164935 [Vibrio cholerae C6706]
MDASIEKRPRTRLSPQKRKLQLMEIALEVFAKRGIGRGGHADIAEIAQVSVATVFNYFPTREDLVDDVLNFVVRQYSNFL
TDHIDLDLDVKTNLQTLCKEMVKLAMTDCHWLKVWFEWSASTRDEVWPLFVSTNRTNQLLIRNMFMKAMERGELCEKHDV
DNMASLFHGIFYSIFLQVNRLGEQEAVYKLADSYLNMLCIYKN*

                                 

Nucleotide sequence of regulator: 612 bp .

                                    >REG0023 NZ_CP046844:c165546-164935 [Vibrio cholerae C6706]
ATGGACGCATCAATCGAAAAACGCCCTCGAACTCGGCTATCGCCTCAAAAACGCAAACTACAACTGATGGAAATCGCGTT
GGAAGTGTTTGCTAAACGCGGCATTGGTCGTGGTGGTCACGCAGATATTGCCGAGATTGCGCAAGTCTCCGTTGCAACAG
TGTTCAACTACTTCCCAACTCGTGAAGACTTGGTTGACGATGTGCTGAATTTTGTGGTTCGTCAGTACTCCAACTTCTTG
ACCGATCACATCGATCTTGATTTGGATGTGAAAACCAACCTACAAACTCTGTGCAAAGAGATGGTGAAATTGGCGATGAC
CGATTGTCACTGGCTCAAAGTCTGGTTTGAGTGGAGTGCTTCAACCCGTGACGAAGTTTGGCCACTGTTTGTTTCCACCA
ACCGAACTAACCAACTGCTGATCAGAAACATGTTTATGAAAGCGATGGAGCGTGGCGAATTGTGTGAGAAACACGATGTC
GATAACATGGCCAGCCTGTTCCACGGCATCTTCTACTCCATCTTCTTACAAGTGAACCGTTTAGGTGAACAAGAAGCAGT
GTATAAGTTGGCCGATAGCTACCTCAATATGCTGTGTATCTATAAGAACTAG

                                