Detailed information of regulator

Summary

Regulator ID	REG0303
Regulator type	protein
Regulatory pathway (from literatures)	c-di-GMP regulatory pathway; QS regulatory pathway; Global regulator
KEGG pathway (determined by BlastKOALA)	Biofilm formation - Escherichia coli (map02026)
Related T6SS	T6SS01112 (Type i1)
Strain	Vibrio cholerae strain A1552
Replicon	chromosome
Sequence	Protein sequence (215 a.a.); Nucleotide sequence (645 bp)
Description	QstR is functional under low c-di-GMP concentration and directly regulates T6SS genes in its regulon by binding to DNA. Use VC0396 in Vibrio cholerae N16961 to describe VCA1552_00154 (QstR) in Vibrio cholerae A1552; QstR is functional under low c-di-GMP concentration and directly regulates T6SS genes in its regulon by binding to DNA; The requirement for VasH in the QstR-dependent T6SS activation showing that VasH is still necessary for TfoX-induced interbacterial killing. T6SS induction is required regulation by the QS regulator QstR. TfoX is able to drive VipA production and T6SS assembly; QstR is required for the TfoX-dependent induction of comEA, comEC and T6SS genes, and that it also acts in concert with HapR
Reference	[1] Jaskólska M, Stutzmann S, Stoudmann C, et al. QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae. Nucleic Acids Res. 2018 Nov 16;46(20):10619-10634. doi: 10.1093/nar/gky717. PMID: 30102403 [2] Metzger LC, Stutzmann S, Scrignari T, et al. Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY. Cell Rep. 2016 May 3;15(5):951-958. doi: 10.1016/j.celrep.2016.03.092. Epub 2016 Apr 21. PMID: 27117415

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External database links

Locus tag (Gene)	VCA1552_00154 (QstR)
Coordinates (Strand)	149920..150564 (-)
NCBI ID	447111060
RefSeq	NZ_CP024867
Uniprot ID	Q9KUW3_VIBCH, A0A0X1L2N3_VIBCO, A0A0H3ALH4_VIBC3, D7HD98_VIBCL, A0A0K9UMZ8_VIBCL, A0A0H3Q739_VIBCL, C3LRC7_VIBCM, A0A0H6R3E4_VIBCL
KEGG ID	vco:VC0395_A2814, vcq:EN18_18975, vch:VC0396, vcm:VCM66_0381
KEGG pathway (determined by BlastKOALA)	map02026
PDB ID	-

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  Target

Target	Regulation (↓/↑)	Target sequence
TssB/TfoY transcripts	↓	-
VC0396/VCA1552_00154 (QstR)	↓	-
T6SS transcripts	↓	-

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Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
GerE	PF00196.21	3e-20	155..208

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-214	MQRANYARTIYLLTTQPKALHPSIQAAIEQLNLPVPVIEPERLLREYQSDKHKILLLDHAENGLIRQQLGPLKLTSPYFETILFNVEKRLRTEDLLTFGNLKGLFYANEDTGFIAHGLGEIINGQNWLPRHVSSQLLHYYRYAFQSHQVQATVDLTAREIQILRCLQTGASNMQIAENLFISEFTVKSHLYQIFKKLNVKNRVKAIAWVNQNLL

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8457	-	-

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  Protein sequence of regulator: 215 a.a.    .Download


  Nucleotide sequence of regulator: 645 bp    .Download