Detailed information of regulator


Summary

Regulator ID REG0093 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Quorum sensing (map02024)
Related T6SS T6SS00040 experimental (Type i1)
Strain Pectobacterium atrosepticum SCRI1043
Replicon chromosome
Sequence Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Description Synthesise AHL, N-(3-oxohexanoyl)-L-homoserine lactone (OHHL).
Reference

External database links

Locus tag (Gene) ECA_RS00520 (expI)
Coordinates (Strand) 125689..126339 (+)
NCBI ID 499404281
RefSeq NC_004547
Uniprot ID Q6DAZ7_PECAS
KEGG ID eca:ECA0105
KEGG pathway (determined by BlastKOALA) map01100; map00270; map02024
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts (ECA4275[hcp1], ECA3428[hcp2] and ECA2866[hcp3]) -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Autoind_synth PF00765.19 2.2e-89 8..187

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-216 MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITGTFFPYFKEINIPDGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSRDKGYDGIYTIVSHPMLTILKRSGWGISVVEQGLSEKEEKVYLVFLPVDDENQEALARRINRSGTFMSNELKQWPLRLPAAIAQA



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9899 - -



  Protein sequence of regulator: 217 a.a.    .

>REG0093 NC_004547:125689-126339 [Pectobacterium atrosepticum SCRI1043]
MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITG
TFFPYFKEINIPDGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSRDKGYDGIYTIVSHPMLTILKRSGWGISV
VEQGLSEKEEKVYLVFLPVDDENQEALARRINRSGTFMSNELKQWPLRLPAAIAQA*

  Nucleotide sequence of regulator: 651 bp    .

>REG0093 NC_004547:125689-126339 [Pectobacterium atrosepticum SCRI1043]
ATGTTAGAGATATTTGATGTAAATCACACCTTGTTGTCAGAAACGAAATCAGAAGAGCTATTTACCCTCAGAAAAGAGAC
GTTTAAAGATCGACTGAATTGGGCCGTGCAATGTACTGACGGAATGGAGTTTGATCAGTATGATAATAATAACACGACTT
ATCTTTTTGGCATAAAAGATAACACCGTTATCTGTAGTTTAAGGTTTATCGAAACAAAATACCCTAACATGATTACTGGA
ACATTTTTCCCTTACTTCAAGGAGATAAATATTCCTGACGGGAATTATCTAGAATCTAGTCGGTTCTTTGTGGATAAATC
ACGGGCGAAAGATATTCTCGGCAATGAATATCCAATTAGTTCGATGTTGTTTCTTTCGATGATTAACTATTCAAGAGATA
AAGGCTATGATGGAATATATACGATAGTGAGTCATCCTATGCTAACGATATTAAAACGTTCTGGCTGGGGAATTAGCGTA
GTTGAACAAGGTCTGTCAGAAAAGGAAGAAAAGGTCTATTTAGTTTTTCTTCCCGTTGATGATGAGAATCAAGAAGCTTT
GGCTCGTCGTATTAACCGCAGTGGGACATTTATGAGCAATGAATTGAAGCAGTGGCCTCTACGGTTACCTGCCGCTATTG
CACAGGCTTGA