SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0093
Regulator type	protein
Regulatory pathway (from literatures)	QS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Quorum sensing (map02024)
Related T6SS	T6SS00040 (Type i1)
Strain	Pectobacterium atrosepticum SCRI1043
Replicon	chromosome
Sequence	Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Description	Synthesise AHL, N-(3-oxohexanoyl)-L-homoserine lactone (OHHL).
Reference	[1] Liu H, Coulthurst SJ, Pritchard L, et al. Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLoS Pathog. 2008 Jun 20;4(6):e1000093. doi: 10.1371/journal.ppat.1000093. PMID: 18566662

External database links

Locus tag (Gene)	ECA_RS00520 (expI)
Coordinates (Strand)	125689..126339 (+)
NCBI ID	499404281
RefSeq	NC_004547
Uniprot ID	Q6DAZ7_PECAS
KEGG ID	eca:ECA0105
KEGG pathway (determined by BlastKOALA)	map01100; map00270; map02024
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS transcripts (ECA4275[hcp1], ECA3428[hcp2] and ECA2866[hcp3])	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Autoind_synth	PF00765.19	2.2e-89	8..187

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-216	MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITGTFFPYFKEINIPDGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSRDKGYDGIYTIVSHPMLTILKRSGWGISVVEQGLSEKEEKVYLVFLPVDDENQEALARRINRSGTFMSNELKQWPLRLPAAIAQA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9899	-	-

Protein sequence of regulator: 217 a.a. .

                                    >REG0093 NC_004547:125689-126339 [Pectobacterium atrosepticum SCRI1043]
MLEIFDVNHTLLSETKSEELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITG
TFFPYFKEINIPDGNYLESSRFFVDKSRAKDILGNEYPISSMLFLSMINYSRDKGYDGIYTIVSHPMLTILKRSGWGISV
VEQGLSEKEEKVYLVFLPVDDENQEALARRINRSGTFMSNELKQWPLRLPAAIAQA*

                                 

Nucleotide sequence of regulator: 651 bp .

                                    >REG0093 NC_004547:125689-126339 [Pectobacterium atrosepticum SCRI1043]
ATGTTAGAGATATTTGATGTAAATCACACCTTGTTGTCAGAAACGAAATCAGAAGAGCTATTTACCCTCAGAAAAGAGAC
GTTTAAAGATCGACTGAATTGGGCCGTGCAATGTACTGACGGAATGGAGTTTGATCAGTATGATAATAATAACACGACTT
ATCTTTTTGGCATAAAAGATAACACCGTTATCTGTAGTTTAAGGTTTATCGAAACAAAATACCCTAACATGATTACTGGA
ACATTTTTCCCTTACTTCAAGGAGATAAATATTCCTGACGGGAATTATCTAGAATCTAGTCGGTTCTTTGTGGATAAATC
ACGGGCGAAAGATATTCTCGGCAATGAATATCCAATTAGTTCGATGTTGTTTCTTTCGATGATTAACTATTCAAGAGATA
AAGGCTATGATGGAATATATACGATAGTGAGTCATCCTATGCTAACGATATTAAAACGTTCTGGCTGGGGAATTAGCGTA
GTTGAACAAGGTCTGTCAGAAAAGGAAGAAAAGGTCTATTTAGTTTTTCTTCCCGTTGATGATGAGAATCAAGAAGCTTT
GGCTCGTCGTATTAACCGCAGTGGGACATTTATGAGCAATGAATTGAAGCAGTGGCCTCTACGGTTACCTGCCGCTATTG
CACAGGCTTGA

                                