Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00115  |
| T6SS type | Type i4b |
| Strain | Burkholderia thailandensis E264 |
| Replicon (RefSeq) | chromosome I (NC_007651) |
| Location | 3396298..3416317 |
| Function | anti-bacterial; effector translocation; adherence |
| Comment | Inactivation of T6SS-1 renders B. thai greatly more susceptible to cell contact-induced stasis by Pseudomonas putida, Pseudomonas fluorescens and Serratia proteamaculans leaving it 100- to 1000-fold less fit than the wild-type in competition experiments with these organisms. |
| References | [1] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068] [2] Schwarz S et al (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170] [3] Lennings J, Makhlouf M, Olejnik P, Mayer C, Brötz-Oesterhelt H, Schwarz S. et al (2019) Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis.. Int J Med Microbiol. 309(6):151335. [PudMed:31378704] [4] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] [5] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TagL | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BTH_RS27245 | 3392425..3393873 | + | 1449 | WP_009888616.1 | TolC family type I secretion outer membrane protein | ||
| BTH_RS27250 | 3394070..3395035 | - | 966 | WP_009910677.1 | hypothetical protein | ||
| BTH_RS27255 | 3395161..3396012 | + | 852 | WP_025369750.1 | M15 family metallopeptidase | ||
| T6CP001085 | TssM | BTH_RS27260 (tssM) | 3396298..3400203 | + | 3906 | WP_011402549.1 | type VI secretion system membrane subunit TssM |
| BTH_RS27265 (tagF) | 3400200..3401189 | + | 990 | WP_009888606.1 | type VI secretion system-associated protein TagF | ||
| T6CP011368 | TagL | BTH_RS27270 | 3401194..3402129 | + | 936 | WP_009888604.1 | OmpA family protein |
| T6CP001086 | TssA | BTH_RS27275 (tssA) | 3402329..3403459 | - | 1131 | WP_009888602.1 | type VI secretion system protein TssA |
| T6CP001087 | TssH | BTH_RS27280 (tssH) | 3403545..3406208 | - | 2664 | WP_009910486.1 | type VI secretion system ATPase TssH |
| T6CP001088 | TssG | BTH_RS27285 (tssG) | 3406242..3407342 | - | 1101 | WP_009888601.1 | type VI secretion system baseplate subunit TssG |
| T6CP001089 | TssF | BTH_RS27290 (tssF) | 3407306..3409144 | - | 1839 | WP_009888599.1 | type VI secretion system baseplate subunit TssF |
| T6CP001090 | TssE | BTH_RS27295 (tssE) | 3409225..3409707 | - | 483 | WP_009888597.1 | type VI secretion system baseplate subunit TssE |
| T6CP001091 | TssD | BTH_RS27300 | 3409765..3410268 | - | 504 | WP_009906860.1 | type VI secretion system tube protein Hcp |
| T6CP001092 | TssC | BTH_RS27305 (tssC) | 3410341..3411831 | - | 1491 | WP_009888594.1 | type VI secretion system contractile sheath large subunit |
| T6CP001093 | TssB | BTH_RS27310 (tssB) | 3411848..3412366 | - | 519 | WP_009888592.1 | type VI secretion system contractile sheath small subunit |
| BTH_RS27315 | 3412403..3413092 | - | 690 | WP_011402551.1 | tetratricopeptide repeat protein | ||
| T6CP001094 | TssJ | BTH_RS27320 (tssJ) | 3413466..3414080 | + | 615 | WP_025404121.1 | type VI secretion system lipoprotein TssJ |
| T6CP001095 | TssK | BTH_RS27325 (tssK) | 3414189..3415535 | + | 1347 | WP_009888581.1 | type VI secretion system baseplate subunit TssK |
| T6CP001096 | TssL | BTH_RS27330 (icmH) | 3415532..3416317 | + | 786 | WP_009888580.1 | type IVB secretion system protein IcmH/DotU |
| BTH_RS27335 | 3416437..3416940 | - | 504 | pseudogene | IS3 family transposase | ||
| BTH_RS34715 | 3416951..3417079 | + | 129 | WP_158338514.1 | transposase | ||
| BTH_RS27345 | 3417661..3418173 | - | 513 | WP_011402553.1 | ClpXP protease specificity-enhancing factor | ||
| BTH_RS27350 | 3418243..3418854 | - | 612 | WP_004185176.1 | glutathione S-transferase N-terminal domain-containing protein | ||
| BTH_RS27355 | 3418947..3419705 | - | 759 | WP_009888567.1 | cytochrome c1 | ||
| BTH_RS27360 | 3419728..3421110 | - | 1383 | WP_009888565.1 | cytochrome bc complex cytochrome b subunit |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0155 |
BTH_II1510 (BtaR1) | protein | QS regulatory pathway | Burkholderia thailandensis E264 | chromosome (NC_007650) | 1779070..1779789 (-) | 497583165 | BTH_I2691/BTH_I2693-99/BTH_I2701-702 (↓) |
Experimental investigation has been performed. Bioinformatics investigation has been performed. Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00071 |
BTH_I0069 (Tai2) | EFF00140 |
Burkholderia thailandensis E264 | chromosome (NC_007651) | 74394..74789 (+) | 83719910 |
| IMU00076 |
BTH_I2699 (Tli1) | EFF00145 |
Burkholderia thailandensis E264 | chromosome (NC_007651) | 3091636..3092235 (-) | 83720426 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|