Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00115 experimental
T6SS type Type i4b
Strain Burkholderia thailandensis E264
Replicon (RefSeq) chromosome I (NC_007651)
Location 3396298..3416317
Function anti-bacterial; effector translocation; adherence
Comment Proliferation in mixed culture with several Gram-negative bacteria, resistance to Pseudomonas putida-induced stasis and persistence in mixed biofilms with P. putida.
References
[1] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806]
[2] Schwarz S et al (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6(8):e1001068. [PudMed:20865170]
[3] Russell AB et al (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 496(7446):508-12. [PudMed:23552891]
[4] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTagLTssATssBTssCTssDTssETssFTssGTssHTssJTssKTssLTssM
Number1111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
BTH_RS27245 3392425..3393873 + 1449 WP_009888616.1 TolC family type I secretion outer membrane protein
BTH_RS27250 3394070..3395035 - 966 WP_009910677.1 hypothetical protein
BTH_RS27255 3395161..3396012 + 852 WP_025369750.1 M15 family metallopeptidase
T6CP001085 TssM BTH_RS27260 (tssM) 3396298..3400203 + 3906 WP_011402549.1 type VI secretion system membrane subunit TssM
BTH_RS27265 (tagF) 3400200..3401189 + 990 WP_009888606.1 type VI secretion system-associated protein TagF
T6CP011368 TagL BTH_RS27270 3401194..3402129 + 936 WP_009888604.1 OmpA family protein
T6CP001086 TssA BTH_RS27275 (tssA) 3402329..3403459 - 1131 WP_009888602.1 type VI secretion system protein TssA
T6CP001087 TssH BTH_RS27280 (tssH) 3403545..3406208 - 2664 WP_009910486.1 type VI secretion system ATPase TssH
T6CP001088 TssG BTH_RS27285 (tssG) 3406242..3407342 - 1101 WP_009888601.1 type VI secretion system baseplate subunit TssG
T6CP001089 TssF BTH_RS27290 (tssF) 3407306..3409144 - 1839 WP_009888599.1 type VI secretion system baseplate subunit TssF
T6CP001090 TssE BTH_RS27295 (tssE) 3409225..3409707 - 483 WP_009888597.1 type VI secretion system baseplate subunit TssE
T6CP001091 TssD BTH_RS27300 3409765..3410268 - 504 WP_009906860.1 type VI secretion system tube protein Hcp
T6CP001092 TssC BTH_RS27305 (tssC) 3410341..3411831 - 1491 WP_009888594.1 type VI secretion system contractile sheath large subunit
T6CP001093 TssB BTH_RS27310 (tssB) 3411848..3412366 - 519 WP_009888592.1 type VI secretion system contractile sheath small subunit
BTH_RS27315 3412403..3413092 - 690 WP_011402551.1 tetratricopeptide repeat protein
T6CP001094 TssJ BTH_RS27320 (tssJ) 3413466..3414080 + 615 WP_025404121.1 type VI secretion system lipoprotein TssJ
T6CP001095 TssK BTH_RS27325 (tssK) 3414189..3415535 + 1347 WP_009888581.1 type VI secretion system baseplate subunit TssK
T6CP001096 TssL BTH_RS27330 (icmH) 3415532..3416317 + 786 WP_009888580.1 type IVB secretion system protein IcmH/DotU
BTH_RS27335 3416437..3416940 - 504 pseudogene IS3 family transposase
BTH_RS34715 3416951..3417079 + 129 WP_158338514.1 transposase
BTH_RS27345 3417661..3418173 - 513 WP_011402553.1 ClpXP protease specificity-enhancing factor
BTH_RS27350 3418243..3418854 - 612 WP_004185176.1 glutathione S-transferase N-terminal domain-containing protein
BTH_RS27355 3418947..3419705 - 759 WP_009888567.1 cytochrome c1
BTH_RS27360 3419728..3421110 - 1383 WP_009888565.1 cytochrome bc complex cytochrome b subunit
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0155 experimental BTH_II1510 (BtaR1) protein QS regulatory pathway Burkholderia thailandensis E264 chromosome (NC_007650) 1779070..1779789 (-) 497583165 BTH_I2691/BTH_I2693-99/BTH_I2701-702 (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF00140 experimental BTH_I0068 (Tae2) Tae IMU00071 experimental Burkholderia thailandensis E264 chromosome (NC_007651) 73967..74407 (+) 83720828
EFF00145 experimental BTH_I2698 (Tle1) Tle Burkholderia thailandensis E264 chromosome (NC_007651) 3089237..3091639 (-) 83721061
EFF00136 experimental BTH_RS00455 Tse - Burkholderia thailandensis E264 chromosome (NC_007650) 100474..102714 (+) 83716109
EFF00137 experimental BTH_RS01610 Tse - Burkholderia thailandensis E264 chromosome (NC_007650) 379615..380514 (-) 83716914
EFF00150 experimental BTH_RS28610 Tse - Burkholderia thailandensis E264 chromosome (NC_007651) 3674112..3676352 (+) 83719543
EFF00142 experimental BTH_RS25940 Tse - Burkholderia thailandensis E264 chromosome (NC_007651) 3077299..3080322 (-) 83720200
EFF00141 experimental BTH_RS21555 Tse - Burkholderia thailandensis E264 chromosome (NC_007651) 2056900..2057376 (-) 83721528
EFF00135 experimental BTH_RS00450 - - Burkholderia thailandensis E264 chromosome (NC_007650) 97680..100460 (+) 83716745
EFF00149 experimental BTH_RS28605 - - Burkholderia thailandensis E264 chromosome (NC_007651) 3671318..3674098 (+) 83718625
EFF00147 experimental BTH_RS26010 - - Burkholderia thailandensis E264 chromosome (NC_007651) 3097448..3100246 (-) 83718826
EFF00144 experimental BTH_RS25975 - - Burkholderia thailandensis E264 chromosome (NC_007651) 3085793..3089128 (-) 83720129
EFF00143 experimental BTH_RS25950 - - Burkholderia thailandensis E264 chromosome (NC_007651) 3081345..3084173 (-) 83718482

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00071 experimental BTH_I0069 (Tai2) EFF00140 experimental Burkholderia thailandensis E264 chromosome (NC_007651) 74394..74789 (+) 83719910
IMU00076 experimental BTH_I2699 (Tli1) EFF00145 experimental Burkholderia thailandensis E264 chromosome (NC_007651) 3091636..3092235 (-) 83720426

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00022 experimental BTH_RS20365 (monT) chaperone Burkholderia thailandensis E264 chromosome (NC_007651) 1796067..1798403 (+) 497575584 - BTH_II1883 (TseM)