Detailed information of component protein


Summary

Component ID T6CP001086
Component protein type TssA
T6SS ID (Type) T6SS00115 experimental (Type i4b)
Strain Burkholderia thailandensis E264
Replicon chromosome I
Sequence Protein sequence (377 a.a.); Nucleotide sequence (1131 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Lennings J, Makhlouf M, Olejnik P, et al. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. Epub 2019 Jul 18. PMID: 31378704
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS27275
Coordinate (Strand) 3402329..3403459 (-)
NCBI ID WP_009888602.1
RefSeq NC_007651
Uniprot ID A0A7X3DR20_BURTH
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 9.6e-37 9..131
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-376 MPINLTELLTPISEASPCGDDLLFSAEFDAIQHARKFDDPSLDQGEWITDIKEADWGFVVEQSSTLLRERTKDLRLAVWLTEALALEDGIGGLTQGYELLTNLCRQFWDHVHPLPDGDDAEYRLGNVAWLAGRTVALLRAVPLTDGASNAFSELDWDVAQHVAQAIRRDPEQANEIARGKPSVEQIEASKRMTPVAFYARLLGELKTFQAALDALEQELDQRAGDAAPSFRQARDAYETVYRLAERFAKEQGYSADAQPRGVAPNAQDASRERAEPSFRTPLHSEEPVQRHAHAPSAPAPIVIAGIQNRAQAVEQLRAVAKYFRGTEPHSPVAYLADKAAEWADMPLHQWLASVVKDDGSLAHIRELLGLKPDDNA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9720 - -


  Protein sequence: 377 a.a.    

>T6CP001086 NC_007651:c3403459-3402329 [Burkholderia thailandensis E264] [TssA]
MPINLTELLTPISEASPCGDDLLFSAEFDAIQHARKFDDPSLDQGEWITDIKEADWGFVVEQSSTLLRERTKDLRLAVWL
TEALALEDGIGGLTQGYELLTNLCRQFWDHVHPLPDGDDAEYRLGNVAWLAGRTVALLRAVPLTDGASNAFSELDWDVAQ
HVAQAIRRDPEQANEIARGKPSVEQIEASKRMTPVAFYARLLGELKTFQAALDALEQELDQRAGDAAPSFRQARDAYETV
YRLAERFAKEQGYSADAQPRGVAPNAQDASRERAEPSFRTPLHSEEPVQRHAHAPSAPAPIVIAGIQNRAQAVEQLRAVA
KYFRGTEPHSPVAYLADKAAEWADMPLHQWLASVVKDDGSLAHIRELLGLKPDDNA*

  Nucleotide sequence: 1131 bp    

>T6CP001086 NC_007651:c3403459-3402329 [Burkholderia thailandensis E264] [TssA]
ATGCCGATCAACCTTACCGAGTTGCTGACGCCGATCAGCGAAGCGTCGCCTTGCGGCGACGATCTCCTGTTCTCCGCCGA
ATTCGACGCGATCCAGCACGCGCGCAAGTTCGACGATCCGTCGCTCGACCAGGGCGAATGGATCACCGACATCAAGGAGG
CGGACTGGGGCTTCGTCGTCGAGCAATCGAGCACGCTGTTGCGCGAGCGCACCAAGGACCTGCGGCTCGCCGTATGGCTG
ACCGAGGCGCTCGCGCTCGAGGACGGCATCGGCGGGCTCACGCAGGGCTACGAACTGCTCACGAACCTCTGCCGGCAATT
CTGGGATCACGTACACCCGCTGCCGGACGGCGACGATGCCGAATACCGGCTCGGCAACGTCGCGTGGCTCGCCGGGCGCA
CGGTCGCGCTGCTGCGCGCGGTGCCGCTTACCGACGGCGCGTCGAACGCGTTCAGCGAGCTCGACTGGGACGTTGCGCAA
CACGTCGCGCAGGCGATCCGGCGCGATCCCGAGCAGGCGAACGAGATCGCGCGCGGCAAGCCGTCGGTCGAGCAGATCGA
GGCGTCGAAGCGGATGACACCCGTTGCGTTCTACGCGCGGCTGCTCGGCGAACTGAAAACGTTCCAGGCGGCGCTCGACG
CGCTCGAGCAGGAACTCGACCAACGCGCGGGCGACGCGGCGCCGAGCTTCCGTCAGGCGCGCGATGCGTACGAAACCGTC
TACCGGCTTGCCGAGCGTTTCGCGAAGGAGCAGGGCTACTCGGCCGATGCGCAGCCGCGGGGCGTCGCGCCGAATGCGCA
GGACGCATCGCGCGAGCGCGCGGAGCCGAGCTTCAGGACGCCGCTTCATTCGGAGGAACCCGTGCAGAGACACGCACACG
CGCCGTCCGCGCCCGCGCCGATCGTGATCGCCGGCATCCAGAATCGCGCGCAAGCGGTCGAGCAACTGCGCGCGGTCGCA
AAGTATTTTCGCGGAACCGAGCCGCACAGCCCGGTTGCGTATCTCGCCGACAAGGCGGCAGAGTGGGCCGACATGCCGCT
GCATCAATGGCTCGCGTCGGTCGTCAAGGACGACGGCTCGCTCGCGCATATCCGCGAGCTGCTCGGCCTGAAGCCGGACG
ACAACGCGTGA