Detailed information of component protein


Summary

Component ID T6CP001085
Component protein type TssM
T6SS ID (Type) T6SS00115 experimental (Type i4b)
Strain Burkholderia thailandensis E264
Replicon chromosome I
Sequence Protein sequence (1302 a.a.); Nucleotide sequence (3906 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Lennings J, Makhlouf M, Olejnik P, et al. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. Epub 2019 Jul 18. PMID: 31378704
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS27260
Coordinate (Strand) 3396298..3400203 (+)
NCBI ID WP_011402549.1
RefSeq NC_007651
Uniprot ID Q2SUD8_BURTA
KEGG ID bte:BTH_I2954
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 9.2e-96 494..862
IcmF-related_N PF14331.8 5.4e-83 190..442
IcmF_C PF06744.14 1.8e-22 1131..1234
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MQRFLNVLTHPR
Transmembrane helix 13-30 TLTIVGFAALAAVLFIAA
Outside 31-34 DALQ
Transmembrane helix 35-57 IGFVWAAGALAVALALWLATKLW
Inside 58-439 RRSRVRRANRQLGDMLEQQAETGKVSAAVAEPAKRADLDVLRTRLADSVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVINSGLQFPFADKNSAVIHGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWLGFLDLLKRYRPKAPINGIIVTASIAELTGNRPEFAINLAKNLRQRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDRQEYDRVWGATLPYEPDEKRDVVALFDQRFEELYDGLKEISVAQLSISRGNKLSPGQLSFPLEFSTIKPALRSFLATLFEDNPFQYKPIFRGFYFTSALQEGETNSAAAQRIANRFGLDSQSLPKPHSAFSKNGFFLRDLFSKVIFADRQTVRQFASPTKTR
Transmembrane helix 440-462 VRYATFFGFVAALALALGGWTWS
Outside 463-1301 TIGNQQLVSNVQADLDNVTRLQQNRNDLQSRLQAMDILEDRIEQLEQFRRDKPLSVSLGLYQGNRLEQHLLTEYYNGVRQILLAPVSDNLASFLKDVNAHPDQLAPMTRAPESGATPAGPMPVSANPAAAPGTAPSPAVAPNQQQGGLYNDASPTNVEDAYNALKTYLMLSDKRHVEQAHLTDQVARFWRGWLETNRGNMPRDEMIKSAERMITFYLSRVSDNDWPMIEANLALVDQTRENLRRVVRGMPARQRVYEEIKARASTRFAPMTIARIVGEGNTGLVAGSYAIPGTFTREAWFQYVQPAIRDAATKELQAKDWVLNTASADDLTLEGSPEQIQKTLVAMYKTEYAQHWQKFMQGIAVQGFGNFTQAVDAMNKLGDPQDSPIRKVLETAYDQTSWDNPSLANATIRKAQTGVVNWVKQWFTRQPGGQIAANVDINGNPADVPMGPIGQEFVGLGRIVATHDGTSMLKGYMDTLSKVRTRFNVIKNQGDPGPGARQLMQQTLDGNGSELADSLKFVDEQMLTGLTDSQRKSLRPLLVRPLMQAFAVVIQPASVEVNKVWNAQVYQPFQNSLANKYPFAAGAKVEAGAGEIAQVFGPDGSIAKFVGATLGPLAVRRGDTLSARTWGDMGLALTPDFTSGFARWVAPLAGGAATSAAGSSEPQTVFQVLPQPSSGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPQGSPGATLSATTFDGRTVQLVNEPGRYGLEKLINSAQRKRRADGTFDLTWTQGSVSVSVTMRIISTSQPTGGGGDQPQQQSLRGLRLPSSVADASAGGAANATAQPGSGGTAAAPQAAVATASASNAQGAQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9917 - -


  Protein sequence: 1302 a.a.    

>T6CP001085 NC_007651:3396298-3400203 [Burkholderia thailandensis E264] [TssM]
MQRFLNVLTHPRTLTIVGFAALAAVLFIAADALQIGFVWAAGALAVALALWLATKLWRRSRVRRANRQLGDMLEQQAETG
KVSAAVAEPAKRADLDVLRTRLADSVKTIKTSKIGQVSGGSALYELPWYIVIGNPAAGKSSAVINSGLQFPFADKNSAVI
HGIGGTRNCDWFFTTEGILLDTAGRYSVHEEDRSEWLGFLDLLKRYRPKAPINGIIVTASIAELTGNRPEFAINLAKNLR
QRVQELTEKLEVFAPVYVMFTKADLITGFTEFFSSSDRQEYDRVWGATLPYEPDEKRDVVALFDQRFEELYDGLKEISVA
QLSISRGNKLSPGQLSFPLEFSTIKPALRSFLATLFEDNPFQYKPIFRGFYFTSALQEGETNSAAAQRIANRFGLDSQSL
PKPHSAFSKNGFFLRDLFSKVIFADRQTVRQFASPTKTRVRYATFFGFVAALALALGGWTWSTIGNQQLVSNVQADLDNV
TRLQQNRNDLQSRLQAMDILEDRIEQLEQFRRDKPLSVSLGLYQGNRLEQHLLTEYYNGVRQILLAPVSDNLASFLKDVN
AHPDQLAPMTRAPESGATPAGPMPVSANPAAAPGTAPSPAVAPNQQQGGLYNDASPTNVEDAYNALKTYLMLSDKRHVEQ
AHLTDQVARFWRGWLETNRGNMPRDEMIKSAERMITFYLSRVSDNDWPMIEANLALVDQTRENLRRVVRGMPARQRVYEE
IKARASTRFAPMTIARIVGEGNTGLVAGSYAIPGTFTREAWFQYVQPAIRDAATKELQAKDWVLNTASADDLTLEGSPEQ
IQKTLVAMYKTEYAQHWQKFMQGIAVQGFGNFTQAVDAMNKLGDPQDSPIRKVLETAYDQTSWDNPSLANATIRKAQTGV
VNWVKQWFTRQPGGQIAANVDINGNPADVPMGPIGQEFVGLGRIVATHDGTSMLKGYMDTLSKVRTRFNVIKNQGDPGPG
ARQLMQQTLDGNGSELADSLKFVDEQMLTGLTDSQRKSLRPLLVRPLMQAFAVVIQPASVEVNKVWNAQVYQPFQNSLAN
KYPFAAGAKVEAGAGEIAQVFGPDGSIAKFVGATLGPLAVRRGDTLSARTWGDMGLALTPDFTSGFARWVAPLAGGAATS
AAGSSEPQTVFQVLPQPSSGTTEYTIAIDGQQLRYRNTPPQWTNFVWPNPQGSPGATLSATTFDGRTVQLVNEPGRYGLE
KLINSAQRKRRADGTFDLTWTQGSVSVSVTMRIISTSQPTGGGGDQPQQQSLRGLRLPSSVADASAGGAANATAQPGSGG
TAAAPQAAVATASASNAQGAQ*

  Nucleotide sequence: 3906 bp    

>T6CP001085 NC_007651:3396298-3400203 [Burkholderia thailandensis E264] [TssM]
ATGCAACGCTTCCTCAACGTGTTGACTCACCCTCGCACGCTCACGATCGTCGGCTTCGCCGCGCTCGCGGCGGTTCTGTT
CATCGCGGCCGATGCGCTACAGATCGGCTTCGTATGGGCGGCCGGCGCGCTCGCCGTCGCGCTCGCGCTCTGGCTCGCGA
CGAAGCTCTGGCGCCGCTCGCGCGTGCGCCGCGCGAATCGCCAGCTCGGCGACATGCTCGAGCAGCAGGCCGAAACGGGC
AAGGTGTCCGCCGCCGTGGCCGAGCCCGCCAAGCGGGCGGACCTCGACGTGCTGCGCACCCGCCTTGCCGACTCCGTGAA
GACGATCAAGACCTCGAAGATCGGCCAGGTGTCGGGCGGCTCCGCGCTGTACGAACTGCCGTGGTACATCGTGATCGGCA
ACCCGGCCGCGGGCAAGAGCAGCGCCGTCATCAACTCGGGGCTGCAGTTTCCGTTCGCGGACAAGAACAGCGCGGTGATC
CACGGCATCGGCGGCACGCGCAACTGCGACTGGTTCTTCACGACGGAAGGCATCCTGCTCGACACCGCGGGCCGTTATTC
GGTGCACGAGGAAGACCGCAGCGAATGGCTCGGCTTCCTCGATCTGCTCAAGCGCTATCGTCCGAAGGCGCCGATCAACG
GGATCATCGTGACGGCGAGCATCGCCGAGCTGACGGGCAACCGTCCCGAATTCGCGATCAACCTCGCGAAGAACCTGCGC
CAGCGCGTGCAGGAGCTCACCGAGAAGCTCGAAGTGTTCGCGCCCGTCTACGTGATGTTCACGAAGGCCGACCTGATCAC
GGGCTTCACCGAGTTCTTCAGCAGCAGCGACCGCCAGGAATACGACCGCGTGTGGGGCGCGACGCTGCCGTACGAGCCGG
ACGAGAAGCGCGATGTCGTCGCGCTGTTCGATCAGCGCTTCGAAGAGCTGTATGACGGGCTGAAGGAGATCAGCGTCGCG
CAACTGTCGATCAGCCGCGGCAACAAGCTTTCGCCCGGCCAACTGAGCTTCCCGCTCGAATTCTCGACGATCAAGCCCGC
GCTGCGCTCGTTCCTCGCGACGCTGTTCGAGGACAACCCGTTCCAGTACAAGCCGATCTTCCGCGGCTTCTATTTCACCA
GCGCGTTGCAGGAAGGCGAAACGAACAGCGCGGCCGCGCAGCGGATCGCGAACCGCTTCGGCCTTGATTCGCAAAGCCTG
CCGAAGCCGCACAGCGCATTCTCGAAGAACGGCTTCTTCCTGCGCGACCTGTTCTCGAAGGTGATCTTCGCGGACCGCCA
GACAGTCCGCCAGTTCGCGAGCCCGACGAAGACGCGCGTGCGCTACGCGACGTTCTTCGGCTTCGTCGCGGCGCTCGCGC
TCGCGCTCGGCGGCTGGACGTGGTCGACGATCGGCAACCAGCAGCTCGTCTCGAACGTGCAAGCCGACCTCGACAACGTG
ACCCGCCTGCAGCAGAACCGCAACGACCTGCAGTCGCGCCTGCAGGCGATGGACATCCTCGAGGACCGGATCGAGCAGCT
CGAGCAGTTCCGCCGCGACAAGCCGCTGTCCGTGTCGCTCGGCCTGTATCAGGGCAACCGGCTCGAGCAGCATCTGCTGA
CCGAGTACTACAACGGCGTGCGGCAGATCCTGCTCGCCCCCGTGTCGGACAACCTCGCCTCGTTCCTGAAGGACGTGAAC
GCGCACCCGGACCAGCTCGCGCCGATGACGCGCGCCCCGGAATCGGGCGCGACGCCCGCAGGCCCGATGCCGGTGTCGGC
GAATCCCGCAGCTGCGCCGGGTACCGCCCCCTCCCCTGCCGTCGCGCCGAACCAGCAACAGGGCGGCCTGTATAACGACG
CGTCGCCGACCAACGTCGAGGACGCGTACAACGCGCTGAAGACCTACCTGATGCTGTCCGACAAGCGGCACGTCGAGCAG
GCGCACCTGACCGATCAGGTCGCGCGCTTCTGGCGCGGCTGGCTCGAGACGAATCGCGGCAACATGCCACGCGACGAGAT
GATCAAGAGCGCGGAGCGGATGATCACGTTCTACCTGTCGCGCGTGTCCGACAACGATTGGCCGATGATCGAGGCGAATC
TCGCGCTCGTCGATCAGACCCGCGAGAACCTGCGCCGCGTCGTGCGCGGGATGCCGGCCCGCCAGCGCGTGTACGAGGAG
ATCAAGGCGCGCGCGTCGACCCGCTTCGCGCCGATGACGATCGCGCGCATCGTCGGCGAAGGCAACACGGGCCTCGTCGC
GGGCAGCTATGCGATCCCCGGCACGTTCACGCGCGAGGCGTGGTTCCAGTACGTGCAGCCGGCGATCCGCGACGCCGCGA
CCAAGGAGCTGCAGGCGAAGGACTGGGTGCTGAACACCGCGTCGGCCGACGATCTGACGCTCGAGGGCAGCCCCGAGCAG
ATCCAGAAGACGCTCGTCGCGATGTACAAGACCGAGTACGCGCAGCACTGGCAGAAGTTCATGCAGGGCATCGCGGTGCA
GGGCTTCGGCAACTTCACGCAGGCCGTCGATGCGATGAACAAGCTCGGCGACCCGCAGGATTCGCCGATCCGCAAGGTGC
TCGAGACCGCGTACGACCAGACCTCGTGGGACAACCCGTCGCTCGCGAACGCGACGATCAGGAAGGCGCAGACGGGCGTC
GTGAACTGGGTCAAGCAGTGGTTCACGCGCCAGCCGGGCGGCCAGATCGCGGCGAACGTCGACATCAACGGCAATCCCGC
CGACGTGCCGATGGGCCCGATCGGCCAGGAGTTCGTCGGGCTCGGCCGGATCGTCGCGACGCACGACGGCACGTCGATGC
TCAAGGGCTACATGGACACGCTGTCGAAGGTCCGCACGCGCTTTAACGTGATCAAGAACCAGGGCGACCCCGGCCCCGGC
GCGCGCCAGCTGATGCAGCAGACGCTCGACGGCAACGGCTCGGAGCTCGCCGATTCGCTGAAGTTCGTCGACGAGCAGAT
GCTCACGGGCCTGACCGATTCGCAACGCAAGTCGCTGCGCCCGCTCCTCGTGCGTCCGCTGATGCAGGCGTTCGCGGTCG
TGATCCAGCCGGCGAGCGTCGAGGTGAACAAGGTGTGGAACGCGCAGGTGTACCAGCCGTTCCAGAACTCGCTCGCGAAC
AAGTATCCGTTCGCGGCGGGCGCGAAGGTCGAAGCGGGCGCGGGCGAGATCGCGCAGGTGTTCGGCCCCGACGGCTCGAT
CGCGAAGTTCGTCGGCGCGACGCTCGGGCCGCTCGCAGTGCGCCGCGGCGACACGCTGTCCGCGCGCACCTGGGGCGACA
TGGGCCTCGCGCTCACGCCGGATTTCACGAGCGGCTTCGCGCGCTGGGTCGCGCCGCTCGCGGGCGGCGCGGCGACGAGC
GCCGCCGGGTCGTCCGAACCGCAGACCGTGTTCCAGGTGCTGCCGCAGCCGAGCAGCGGCACGACCGAGTACACGATCGC
GATCGACGGCCAGCAACTGCGTTACCGCAACACGCCGCCGCAATGGACGAACTTCGTGTGGCCGAACCCGCAGGGCTCGC
CCGGCGCGACGCTGTCCGCCACGACGTTCGACGGCCGTACGGTGCAGCTCGTCAACGAGCCGGGCCGCTACGGTCTCGAG
AAGCTGATCAACTCGGCGCAGCGCAAGCGCCGTGCGGACGGCACGTTCGATCTGACGTGGACGCAAGGCAGCGTCAGCGT
GTCGGTGACGATGCGCATCATCAGCACGTCGCAGCCGACTGGCGGCGGCGGCGATCAGCCGCAGCAGCAGAGCCTGCGCG
GCCTGCGCCTGCCGTCGTCGGTCGCCGACGCGAGCGCGGGCGGCGCGGCCAACGCAACGGCGCAGCCCGGCTCGGGCGGC
ACGGCCGCGGCCCCGCAGGCCGCCGTCGCGACGGCTTCCGCATCGAATGCACAGGGGGCGCAATGA