SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00140
Effector type	Tae
Cognate immunity protein	IMU00071
Related T6SS (Type)	T6SS00115 (Type i4b)
Strain	Burkholderia thailandensis E264
Replicon	chromosome
Sequence	Protein sequence (147 a.a.); Nucleotide sequence (441 bp)
Description	Tae2; amidase
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BTH_I0068 (Tae2)
Coordinates (Strand)	73967..74407 (+)
NCBI ID	83720828
RefSeq	NC_007651
Uniprot ID	UPI0000668460
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-146	MPYVSTHYSNNAGAPVGRWTCAPTSKLAPFDKAPTGSVTSGVDLCGQCVSYVKRVCPTLPLTGQWRKGAPVKGNATIVAGTVIATFNAAGKYDGHAAIYVSQTKDGGILVYDQFVTPPTPQPVQQRRLRWGAHGRSNNGDNFYVVE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8104	-	-

Protein sequence: 147 a.a. .

                                    >EFF00140 NC_007651:73967-74407 Type VI Amidase Effector (Tae) [Burkholderia thailandensis E264]
MPYVSTHYSNNAGAPVGRWTCAPTSKLAPFDKAPTGSVTSGVDLCGQCVSYVKRVCPTLPLTGQWRKGAPVKGNATIVAG
TVIATFNAAGKYDGHAAIYVSQTKDGGILVYDQFVTPPTPQPVQQRRLRWGAHGRSNNGDNFYVVE*

                                

Nucleotide sequence: 441 bp .

                                    >EFF00140 NC_007651:73967-74407 Type VI Amidase Effector (Tae) [Burkholderia thailandensis E264]
ATGCCTTACGTGTCGACTCATTACTCGAACAACGCCGGCGCGCCGGTCGGCCGCTGGACCTGCGCGCCGACTTCGAAGCT
CGCGCCTTTCGACAAGGCGCCGACGGGCAGCGTCACGTCCGGCGTCGATCTGTGCGGCCAATGCGTGTCCTACGTGAAAC
GGGTCTGCCCGACGCTGCCGCTCACCGGGCAATGGCGCAAGGGCGCGCCGGTGAAAGGCAACGCGACGATCGTCGCGGGC
ACCGTGATCGCGACGTTCAACGCCGCCGGCAAGTACGACGGCCATGCGGCGATCTACGTCAGCCAGACGAAGGACGGCGG
CATCCTGGTGTACGACCAGTTCGTGACGCCGCCGACGCCGCAGCCGGTGCAGCAACGGCGCCTGCGCTGGGGCGCGCATG
GCCGGTCGAACAATGGGGACAACTTCTATGTCGTGGAGTAA

                                