SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00145
Effector type	Tle
Cognate immunity protein	IMU00076
Related T6SS (Type)	T6SS00115 (Type i4b)
Strain	Burkholderia thailandensis E264
Replicon	chromosome
Sequence	Protein sequence (801 a.a.); Nucleotide sequence (2403 bp)
Description	Tle1; Lipase
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806 [2] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BTH_I2698 (Tle1)
Coordinates (Strand)	3089237..3091639 (-)
NCBI ID	83721061
RefSeq	NC_007651
Uniprot ID	UPI0000668539
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DUF2235	PF09994.11	4e-18	295..407
DUF2235	PF09994.11	4.7e-10	42..280

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-800	MNFRFAPAERPDIGVVTKEEKDAILRRLHDDDGMSCCKTLHIGIFFDGTRNNAERDKSGHKHSNVARLRDAFPQDRYHKSIYVAGVGTPFSSEIGDYGIGLQAVAGASAGWAGEGRINWALLQIHNAVHECAFRVGLSTALGVDDKNLVKLMSLDMNFKGIDLGGNAPQPGSTGDIKSRSSPGIGALKLIAAEQYGAELTWDKDTNWSQLKDDLDSSKWAAAVRAWDGRRRKILGDRRAQLKARVGDMLVKGKPRIQRIRLYVFGFSRGAAEARTFSNWLVDALESDFSLCGVPVSYDFLGIFDTVASVGIAQSAAATLFDGHGGWARKELMAVPHYVRRCVHMVAAHEPRGSFPLDLIDCSLEGREEIVYPGVHSDVGGGYGPAEQGRGRGDADKLSQVPLVDMYRAARIAGVPLDIQGPGITSEAADVFKISAGLKQAFTAYVKASEGYYYAKEHGTAGLMRAHYGLYLRWRRMRLKDMSLQPSFKAAQANCPQDAMDIDSANKELRAEWEDLLEIEKEGGPSVAHYAKKFGAKVARDNPKIVASVSAVLLPGVIVFSTRPEVIYGVRKAGDRVTELVRAQLQEKWEQWQQVRSDWNMGPPEAPISALYDNYMHDSRAWFKPLGDDDDVWNYKQIQELKSKQASFEREHAAWRKRAETGAPGPWQIAQAMSAGASGLGPIAMQPEPEPRSPLTAQQADLLKRYDAAMQSAKQARAAKDPNAPTDSAVLTDPKVTGGLALQTSGREFYFLWGFLRWRTVFVNGVRWDQPRVPTVQEEMEGMRMQMQRQVDMKGIGVLFQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9862	-	-

Protein sequence: 801 a.a. .

                                    >EFF00145 NC_007651:c3091639-3089237 Type VI Lipase Effector (Tle) [Burkholderia thailandensis E264]
MNFRFAPAERPDIGVVTKEEKDAILRRLHDDDGMSCCKTLHIGIFFDGTRNNAERDKSGHKHSNVARLRDAFPQDRYHKS
IYVAGVGTPFSSEIGDYGIGLQAVAGASAGWAGEGRINWALLQIHNAVHECAFRVGLSTALGVDDKNLVKLMSLDMNFKG
IDLGGNAPQPGSTGDIKSRSSPGIGALKLIAAEQYGAELTWDKDTNWSQLKDDLDSSKWAAAVRAWDGRRRKILGDRRAQ
LKARVGDMLVKGKPRIQRIRLYVFGFSRGAAEARTFSNWLVDALESDFSLCGVPVSYDFLGIFDTVASVGIAQSAAATLF
DGHGGWARKELMAVPHYVRRCVHMVAAHEPRGSFPLDLIDCSLEGREEIVYPGVHSDVGGGYGPAEQGRGRGDADKLSQV
PLVDMYRAARIAGVPLDIQGPGITSEAADVFKISAGLKQAFTAYVKASEGYYYAKEHGTAGLMRAHYGLYLRWRRMRLKD
MSLQPSFKAAQANCPQDAMDIDSANKELRAEWEDLLEIEKEGGPSVAHYAKKFGAKVARDNPKIVASVSAVLLPGVIVFS
TRPEVIYGVRKAGDRVTELVRAQLQEKWEQWQQVRSDWNMGPPEAPISALYDNYMHDSRAWFKPLGDDDDVWNYKQIQEL
KSKQASFEREHAAWRKRAETGAPGPWQIAQAMSAGASGLGPIAMQPEPEPRSPLTAQQADLLKRYDAAMQSAKQARAAKD
PNAPTDSAVLTDPKVTGGLALQTSGREFYFLWGFLRWRTVFVNGVRWDQPRVPTVQEEMEGMRMQMQRQVDMKGIGVLFQ
*

                                

Nucleotide sequence: 2403 bp .

                                    >EFF00145 NC_007651:c3091639-3089237 Type VI Lipase Effector (Tle) [Burkholderia thailandensis E264]
ATGAATTTTCGCTTTGCTCCGGCCGAACGCCCCGACATAGGCGTTGTGACGAAAGAAGAAAAGGACGCGATTCTTCGGCG
GCTTCATGACGACGATGGTATGAGTTGTTGTAAAACTCTTCACATCGGAATTTTCTTCGACGGTACGCGTAACAACGCGG
AAAGGGACAAGTCAGGGCATAAGCACAGTAACGTGGCTCGCCTTCGGGACGCGTTTCCTCAAGATCGATATCACAAGTCC
ATTTACGTCGCTGGGGTAGGGACACCTTTCTCAAGCGAAATCGGTGATTATGGTATCGGACTTCAAGCCGTGGCTGGCGC
ATCTGCCGGATGGGCTGGCGAAGGACGTATTAACTGGGCCTTATTGCAAATCCATAATGCAGTGCACGAATGTGCTTTCC
GTGTTGGTCTTAGTACCGCGCTCGGGGTGGACGACAAGAATTTGGTGAAGTTGATGAGCCTAGACATGAACTTCAAGGGC
ATCGACCTGGGTGGAAATGCTCCCCAACCTGGTAGTACAGGTGACATTAAATCGCGATCGAGCCCTGGCATCGGTGCGCT
CAAGTTGATCGCGGCCGAACAGTACGGCGCGGAGTTGACGTGGGACAAGGATACGAACTGGTCCCAGTTAAAGGATGATC
TGGATTCGTCGAAATGGGCGGCGGCTGTGCGGGCGTGGGACGGTAGACGCCGCAAGATTTTGGGCGATCGTCGTGCGCAG
TTGAAGGCGCGGGTCGGCGACATGCTTGTTAAGGGCAAGCCGAGAATCCAGCGCATTCGCCTTTATGTGTTTGGATTTTC
GCGGGGTGCAGCGGAAGCTCGCACGTTTTCGAATTGGCTTGTCGATGCACTCGAATCCGATTTCAGCCTGTGTGGTGTTC
CCGTCTCGTACGATTTCCTTGGAATTTTCGATACTGTCGCGTCAGTCGGCATTGCGCAGAGCGCCGCCGCGACACTTTTC
GATGGGCACGGCGGTTGGGCGCGTAAGGAATTAATGGCGGTGCCGCACTATGTCCGCCGCTGCGTGCATATGGTCGCTGC
GCATGAGCCGCGCGGCTCGTTTCCGCTCGATCTGATCGACTGTTCGTTGGAAGGGCGAGAGGAAATTGTGTATCCCGGCG
TTCACTCAGATGTGGGGGGTGGTTACGGACCAGCCGAGCAGGGACGAGGACGAGGCGATGCCGATAAGTTGTCGCAAGTT
CCACTCGTTGACATGTATAGAGCGGCACGTATCGCCGGCGTACCGCTCGATATACAAGGACCGGGCATCACGTCGGAAGC
GGCCGATGTATTTAAGATCTCCGCTGGACTCAAGCAAGCCTTTACTGCCTATGTCAAGGCAAGTGAGGGCTACTATTACG
CGAAGGAGCATGGGACAGCGGGTTTGATGCGAGCTCATTATGGTTTGTATCTGCGCTGGCGCAGGATGCGTCTCAAGGAT
ATGAGCTTGCAGCCGAGTTTCAAGGCTGCGCAGGCGAATTGTCCGCAAGACGCTATGGATATCGACAGTGCGAATAAGGA
ATTAAGGGCGGAATGGGAAGATCTGCTGGAGATTGAAAAGGAGGGAGGCCCGAGTGTGGCCCATTATGCAAAGAAGTTTG
GAGCGAAAGTGGCTCGAGACAATCCTAAAATTGTCGCATCCGTCTCGGCCGTTCTTTTGCCGGGGGTCATCGTCTTCAGT
ACTCGTCCTGAAGTTATTTACGGGGTGAGAAAGGCCGGTGATCGCGTGACAGAACTAGTCCGCGCCCAGCTTCAAGAAAA
ATGGGAGCAATGGCAGCAAGTCCGCAGCGACTGGAACATGGGCCCGCCCGAAGCGCCGATCAGCGCGCTGTACGACAACT
ACATGCACGACTCGCGAGCATGGTTCAAGCCGCTCGGCGATGACGACGACGTCTGGAACTACAAGCAGATCCAGGAGCTG
AAGTCGAAGCAAGCGAGCTTCGAGCGCGAGCACGCCGCATGGAGAAAACGCGCCGAAACGGGCGCGCCGGGGCCGTGGCA
GATCGCGCAGGCGATGAGTGCGGGGGCGTCCGGACTCGGGCCGATCGCAATGCAGCCCGAACCCGAACCCCGCTCCCCGC
TCACCGCCCAGCAAGCCGATTTGCTCAAGCGCTACGACGCCGCGATGCAGTCCGCGAAGCAGGCGCGCGCCGCGAAGGAC
CCGAACGCGCCGACGGACAGCGCCGTGCTCACCGATCCGAAAGTGACGGGCGGCCTCGCGTTGCAAACCAGCGGCCGCGA
GTTCTATTTCCTGTGGGGCTTCCTGCGCTGGCGAACCGTGTTCGTGAACGGCGTGCGCTGGGACCAGCCGCGCGTGCCGA
CAGTGCAGGAGGAAATGGAGGGCATGCGCATGCAGATGCAACGCCAGGTCGACATGAAGGGCATCGGCGTACTGTTCCAA
TGA

                                