Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00076  |
| Cognate effector | EFF00145 |
| Related T6SS (Type) | T6SS00115 (Type i4b) |
| Strain | Burkholderia thailandensis E264 |
| Replicon | chromosome |
| Sequence | Protein sequence (200 a.a.); Nucleotide sequence (600 bp) |
| Description | Tli1 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BTH_I2699 (Tli1) |
| Coordinates (Strand) | 3091636..3092235 (-) |
| NCBI ID | 83720426 |
| RefSeq | NC_007651 |
| Uniprot ID | UPI00006682DE |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF3304 | PF11745.10 | 9.4e-23 | 60..154 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-9 | MNLHFGSMK |
| Transmembrane helix | 10-27 | IFIRLAVFLMVFLVSTGC |
| Inside | 28-199 | SRAIDHSSENAIAGNESVAQSGDDMMSLKLDALNYTDIPVGTFYVDGVWGGNVFSRIGSGGGGITCCVSLPKKWHPGLSVTVEWRNDEMYKKNPESVASRTVPVEKYGEFTGGYLWVLFFPGEGIKVYATPWLPGAADFPEGLQAPSEACPGDFTLLNSDSRCKNPDKRIKP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9871 | pos: 26-27. STG-CS. | MNLHFGSMKIFIRLAVFLMVFLVSTG |
Protein sequence: 200 a.a. .
>IMU00076 NC_007651:c3092235-3091636 [Burkholderia thailandensis E264]
MNLHFGSMKIFIRLAVFLMVFLVSTGCSRAIDHSSENAIAGNESVAQSGDDMMSLKLDALNYTDIPVGTFYVDGVWGGNV
FSRIGSGGGGITCCVSLPKKWHPGLSVTVEWRNDEMYKKNPESVASRTVPVEKYGEFTGGYLWVLFFPGEGIKVYATPWL
PGAADFPEGLQAPSEACPGDFTLLNSDSRCKNPDKRIKP*
MNLHFGSMKIFIRLAVFLMVFLVSTGCSRAIDHSSENAIAGNESVAQSGDDMMSLKLDALNYTDIPVGTFYVDGVWGGNV
FSRIGSGGGGITCCVSLPKKWHPGLSVTVEWRNDEMYKKNPESVASRTVPVEKYGEFTGGYLWVLFFPGEGIKVYATPWL
PGAADFPEGLQAPSEACPGDFTLLNSDSRCKNPDKRIKP*
Nucleotide sequence: 600 bp .
>IMU00076 NC_007651:c3092235-3091636 [Burkholderia thailandensis E264]
ATGAATTTGCATTTCGGCTCGATGAAGATTTTTATCAGACTGGCGGTATTTCTGATGGTGTTTCTTGTGAGCACAGGTTG
TTCGCGCGCGATAGATCATTCGTCCGAGAATGCCATCGCGGGAAATGAGTCGGTTGCGCAGTCGGGCGACGACATGATGA
GTTTGAAGCTGGATGCGCTCAATTATACGGACATACCTGTTGGAACTTTTTACGTTGATGGCGTATGGGGTGGTAACGTG
TTTTCCCGGATCGGAAGTGGTGGCGGGGGCATCACCTGCTGTGTGTCTTTGCCAAAAAAGTGGCATCCGGGGCTTAGTGT
CACGGTGGAGTGGCGTAACGACGAGATGTATAAGAAAAACCCGGAATCGGTGGCTAGTAGAACTGTGCCAGTGGAAAAAT
ACGGCGAATTTACGGGCGGATACCTGTGGGTTCTGTTCTTCCCGGGTGAAGGAATCAAGGTATATGCAACACCGTGGCTT
CCGGGGGCTGCAGACTTTCCCGAGGGGCTGCAAGCGCCAAGTGAGGCATGTCCAGGAGATTTTACCTTGCTGAACAGTGA
TTCACGATGCAAGAATCCTGACAAAAGGATCAAGCCATGA
ATGAATTTGCATTTCGGCTCGATGAAGATTTTTATCAGACTGGCGGTATTTCTGATGGTGTTTCTTGTGAGCACAGGTTG
TTCGCGCGCGATAGATCATTCGTCCGAGAATGCCATCGCGGGAAATGAGTCGGTTGCGCAGTCGGGCGACGACATGATGA
GTTTGAAGCTGGATGCGCTCAATTATACGGACATACCTGTTGGAACTTTTTACGTTGATGGCGTATGGGGTGGTAACGTG
TTTTCCCGGATCGGAAGTGGTGGCGGGGGCATCACCTGCTGTGTGTCTTTGCCAAAAAAGTGGCATCCGGGGCTTAGTGT
CACGGTGGAGTGGCGTAACGACGAGATGTATAAGAAAAACCCGGAATCGGTGGCTAGTAGAACTGTGCCAGTGGAAAAAT
ACGGCGAATTTACGGGCGGATACCTGTGGGTTCTGTTCTTCCCGGGTGAAGGAATCAAGGTATATGCAACACCGTGGCTT
CCGGGGGCTGCAGACTTTCCCGAGGGGCTGCAAGCGCCAAGTGAGGCATGTCCAGGAGATTTTACCTTGCTGAACAGTGA
TTCACGATGCAAGAATCCTGACAAAAGGATCAAGCCATGA