Detailed information of component protein


Summary

Component ID T6CP001096
Component protein type TssL
T6SS ID (Type) T6SS00115 experimental (Type i4b)
Strain Burkholderia thailandensis E264
Replicon chromosome I
Sequence Protein sequence (262 a.a.); Nucleotide sequence (786 bp)
Reference
[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[2] Schwarz S, West TE, Boyer F, et al. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 2010 Aug 26;6(8):e1001068. doi: 10.1371/journal.ppat.1001068. PMID: 20865170
[3] Russell AB, LeRoux M, Hathazi K, et al. Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013 Apr 25;496(7446):508-12. doi: 10.1038/nature12074. Epub 2013 Apr 3. PMID: 23552891
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
[5] Lennings J, Makhlouf M, Olejnik P, et al. Environmental and cellular factors affecting the localization of T6SS proteins in Burkholderia thailandensis. Int J Med Microbiol. 2019 Sep;309(6):151335. doi: 10.1016/j.ijmm.2019.151335. Epub 2019 Jul 18. PMID: 31378704
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BTH_RS27330
Coordinate (Strand) 3415532..3416317 (+)
NCBI ID WP_009888580.1
RefSeq NC_007651
Uniprot ID Q2SUC4_BURTA, A0A1B4JS37_BURTH
KEGG ID bte:BTH_I2968, btha:DR62_839
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.2e-58 32..230
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-209 MSYAPSLFGGNTPPPAPHTVSSTDAGFQARSLVDLLYDGFFMLFLLKNGREPNDASEFSTRIQEFLGEFERGAKKLNIAADDVYAAKFAFCAAIDESVLSSQFKIRADWERRPLQLVLFGEQLAGEKFYQYLEECRAQGAARLQSLEVFHMCLLLGFQGKYLLEGPEKLAYLTARLGDEIANMKGKRAPFAPHWPLPDQISHRLKREVP
Transmembrane helix 210-232 LWAIGAVFALVGLLAFVGLNTYL
Inside 233-261 KDSTVRALAPYSQVIKVGPESANLTISLP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9730 - -


  Protein sequence: 262 a.a.    

>T6CP001096 NC_007651:3415532-3416317 [Burkholderia thailandensis E264] [TssL]
MSYAPSLFGGNTPPPAPHTVSSTDAGFQARSLVDLLYDGFFMLFLLKNGREPNDASEFSTRIQEFLGEFERGAKKLNIAA
DDVYAAKFAFCAAIDESVLSSQFKIRADWERRPLQLVLFGEQLAGEKFYQYLEECRAQGAARLQSLEVFHMCLLLGFQGK
YLLEGPEKLAYLTARLGDEIANMKGKRAPFAPHWPLPDQISHRLKREVPLWAIGAVFALVGLLAFVGLNTYLKDSTVRAL
APYSQVIKVGPESANLTISLP*

  Nucleotide sequence: 786 bp    

>T6CP001096 NC_007651:3415532-3416317 [Burkholderia thailandensis E264] [TssL]
ATGAGTTACGCGCCTTCCCTCTTCGGCGGCAACACGCCGCCTCCCGCGCCGCACACGGTGTCGTCGACCGACGCCGGCTT
CCAGGCACGCTCGCTCGTCGATCTGCTGTACGACGGCTTCTTCATGCTGTTCCTGCTGAAGAACGGCCGCGAACCGAACG
ACGCGTCGGAATTCAGCACGCGCATTCAGGAGTTCCTGGGCGAATTCGAGCGCGGCGCGAAGAAGCTCAACATCGCCGCC
GACGACGTCTACGCGGCGAAGTTCGCGTTTTGCGCGGCGATCGACGAATCGGTGCTGTCGTCGCAGTTCAAGATCCGCGC
GGATTGGGAACGCCGGCCGCTGCAACTCGTGCTGTTCGGCGAACAGCTTGCGGGCGAGAAGTTCTATCAGTACCTCGAAG
AATGCCGCGCGCAGGGCGCGGCACGGCTGCAGTCGCTCGAGGTGTTCCATATGTGCCTGCTGCTCGGCTTCCAGGGCAAG
TATCTGCTCGAAGGGCCGGAGAAGCTCGCGTATCTGACCGCGCGGCTCGGCGACGAGATCGCGAACATGAAGGGCAAGCG
CGCGCCGTTCGCGCCGCATTGGCCACTTCCGGATCAGATCTCGCACCGGCTCAAGCGCGAGGTGCCGCTGTGGGCGATCG
GGGCGGTGTTCGCGCTCGTCGGGCTGCTGGCGTTCGTCGGGTTGAATACGTATTTGAAGGACAGCACCGTGCGGGCGCTC
GCGCCTTATTCGCAGGTCATCAAGGTTGGGCCGGAGTCGGCTAATTTGACGATTTCGTTGCCTTGA