SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00137
Effector type	Tse
Cognate immunity protein	-
Related T6SS (Type)	T6SS00115 (Type i4b)
Strain	Burkholderia thailandensis E264
Replicon	chromosome
Sequence	Protein sequence (300 a.a.); Nucleotide sequence (900 bp)
Description	-
Reference	[1] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BTH_RS01610
Coordinates (Strand)	379615..380514 (-)
NCBI ID	83716914
RefSeq	NC_007650
Uniprot ID	UPI00006675B5
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-4	MKKY
Transmembrane helix	5-27	ALALLLMLFSSMSAMAGAIIPSG
Outside	28-299	GTLVAGQYVISNNGNFAFGIDAATGKLYFHYKFPTQAWGASSMSQIYGTKPEGALYVGKGDRLVMQTDGRLVFYAGNDVVWSNWYFGPAPIPGSYAVVEDFGVVAIYPPQGGYPSFTWPFASITPNSGALTTVAGYPFGLNFPVLNGYQSLQVFNNVTHYLWNKSFAADSVAMQPDGNLVVYNKGGPVWSTGTSGNPGAYMLVTPVGFVLGKQYGGIVANVPYPVSVDQSSRASGPDDPKPATPPPPALNLPINASWKCYYVRDWLVCYAPG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9966	pos: 20-21. AMA-GA.	MKKYALALLLMLFSSMSAMA

Protein sequence: 300 a.a. .

                                    >EFF00137 NC_007650:c380514-379615 Type VI Secretion System Effector (Tse) [Burkholderia thailandensis E264]
MKKYALALLLMLFSSMSAMAGAIIPSGGTLVAGQYVISNNGNFAFGIDAATGKLYFHYKFPTQAWGASSMSQIYGTKPEG
ALYVGKGDRLVMQTDGRLVFYAGNDVVWSNWYFGPAPIPGSYAVVEDFGVVAIYPPQGGYPSFTWPFASITPNSGALTTV
AGYPFGLNFPVLNGYQSLQVFNNVTHYLWNKSFAADSVAMQPDGNLVVYNKGGPVWSTGTSGNPGAYMLVTPVGFVLGKQ
YGGIVANVPYPVSVDQSSRASGPDDPKPATPPPPALNLPINASWKCYYVRDWLVCYAPG*

                                

Nucleotide sequence: 900 bp .

                                    >EFF00137 NC_007650:c380514-379615 Type VI Secretion System Effector (Tse) [Burkholderia thailandensis E264]
ATGAAGAAATACGCATTGGCGCTCTTGTTGATGCTGTTTTCGTCGATGTCCGCAATGGCGGGGGCGATCATTCCATCGGG
CGGCACGCTGGTGGCGGGACAATACGTGATATCGAACAATGGCAATTTCGCATTCGGCATCGACGCGGCAACCGGCAAGC
TCTATTTCCACTACAAGTTCCCGACGCAGGCGTGGGGCGCAAGTTCGATGTCGCAAATCTACGGAACGAAGCCCGAGGGC
GCGCTCTACGTCGGCAAAGGCGACCGGCTCGTGATGCAGACCGACGGCCGGCTCGTGTTCTACGCGGGCAACGACGTCGT
CTGGAGCAACTGGTATTTCGGCCCCGCGCCGATTCCGGGCTCGTATGCCGTCGTCGAGGATTTCGGCGTCGTCGCGATCT
ATCCGCCGCAGGGCGGCTATCCTTCGTTCACCTGGCCGTTCGCATCGATCACGCCGAACAGCGGCGCGCTCACGACAGTC
GCCGGCTATCCGTTCGGCCTGAACTTCCCGGTGCTCAACGGATACCAATCGCTGCAGGTCTTCAACAACGTCACCCATTA
CCTGTGGAACAAGAGCTTCGCGGCGGACAGCGTCGCGATGCAGCCCGACGGCAATCTCGTCGTGTACAACAAGGGAGGGC
CGGTCTGGTCGACGGGAACGTCGGGCAATCCGGGCGCCTACATGCTGGTGACGCCTGTCGGCTTCGTGCTCGGCAAGCAG
TACGGCGGCATCGTCGCGAACGTGCCGTATCCGGTGTCCGTTGACCAGTCGAGCCGCGCGAGCGGCCCGGACGATCCGAA
GCCCGCCACGCCGCCGCCTCCTGCGCTGAACCTGCCGATCAACGCGAGCTGGAAGTGCTATTACGTCAGGGACTGGCTCG
TGTGCTACGCGCCTGGTTGA

                                