Detailed information of regulator
Summary
| Regulator ID | REG0022  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01168 (Type i1) |
| Strain | Vibrio cholerae C6706 |
| Replicon | chromosome |
| Sequence | Protein sequence (203 a.a.); Nucleotide sequence (609 bp) |
| Description | T6SS-mediated killing are positively regulated by CytR, TfoX, HapR, and QstR. |
| Reference |
External database links
| Locus tag (Gene) | GPY04_RS03590 (TfoX) |
| Coordinates (Strand) | 777344..777952 (+) |
| NCBI ID | 487807468 |
| RefSeq | NZ_CP046844 |
| Uniprot ID | Q9KSV3_VIBCH, A0A0X1KVV8_VIBCO, A0A0H3AJZ6_VIBC3, D7H802_VIBCL, A0A0H3PX92_VIBCL, C3LLK0_VIBCM, A0A2K2UTX7_VIBCL |
| KEGG ID | vcm:VCM66_1109, vch:VC1153, vco:VC0395_A0723 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TfoX_C | PF04994.15 | 2.3e-28 | 119..197 |
| TfoX_N | PF04993.15 | 4.3e-22 | 16..106 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-202 | VIKGSMDMNEQQFFDYVTKFGAYQKRSMFGGIGLFQHDAMYVLVSEDRIFVRGGEELDPELLALGCEKYRHVKKQTTATVNYYDITELYEQHHPELDSIIERSIQFSVNQREFQRSAASRRLRDLPNMQLTLERMVKKAGIDDVETFMSLGAPEVFNKVRQAYGSDVDVKLLWKFAGAIEGIHWKLLQEPRKRQLLESCQQR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9949 | - | - |
Protein sequence of regulator: 203 a.a. .
>REG0022 NZ_CP046844:777344-777952 [Vibrio cholerae C6706]
VIKGSMDMNEQQFFDYVTKFGAYQKRSMFGGIGLFQHDAMYVLVSEDRIFVRGGEELDPELLALGCEKYRHVKKQTTATV
NYYDITELYEQHHPELDSIIERSIQFSVNQREFQRSAASRRLRDLPNMQLTLERMVKKAGIDDVETFMSLGAPEVFNKVR
QAYGSDVDVKLLWKFAGAIEGIHWKLLQEPRKRQLLESCQQR*
VIKGSMDMNEQQFFDYVTKFGAYQKRSMFGGIGLFQHDAMYVLVSEDRIFVRGGEELDPELLALGCEKYRHVKKQTTATV
NYYDITELYEQHHPELDSIIERSIQFSVNQREFQRSAASRRLRDLPNMQLTLERMVKKAGIDDVETFMSLGAPEVFNKVR
QAYGSDVDVKLLWKFAGAIEGIHWKLLQEPRKRQLLESCQQR*
Nucleotide sequence of regulator: 609 bp .
>REG0022 NZ_CP046844:777344-777952 [Vibrio cholerae C6706]
GTGATTAAAGGATCAATGGATATGAATGAGCAACAGTTTTTCGACTACGTAACTAAGTTTGGCGCCTACCAAAAACGCTC
GATGTTTGGTGGTATTGGCTTGTTTCAACACGACGCTATGTATGTGTTGGTCAGTGAAGACCGCATTTTTGTGCGTGGTG
GAGAAGAGCTCGACCCTGAGCTCTTGGCCTTAGGGTGCGAGAAATATCGCCATGTTAAAAAACAGACAACGGCAACCGTA
AACTATTACGACATCACTGAACTGTATGAGCAACATCACCCTGAGCTAGACTCGATTATTGAACGCTCGATTCAGTTTTC
TGTGAATCAGCGGGAATTTCAACGCTCAGCAGCCAGTCGTCGTCTACGGGATTTACCCAATATGCAACTGACTTTGGAGC
GTATGGTAAAAAAAGCGGGCATTGACGATGTGGAAACTTTTATGAGCCTCGGTGCGCCAGAAGTGTTTAATAAGGTGCGT
CAAGCTTATGGAAGTGATGTCGATGTAAAACTACTGTGGAAGTTTGCGGGTGCGATTGAAGGCATTCACTGGAAACTGCT
GCAAGAGCCGCGTAAGCGCCAACTGTTAGAAAGTTGTCAGCAGCGTTAA
GTGATTAAAGGATCAATGGATATGAATGAGCAACAGTTTTTCGACTACGTAACTAAGTTTGGCGCCTACCAAAAACGCTC
GATGTTTGGTGGTATTGGCTTGTTTCAACACGACGCTATGTATGTGTTGGTCAGTGAAGACCGCATTTTTGTGCGTGGTG
GAGAAGAGCTCGACCCTGAGCTCTTGGCCTTAGGGTGCGAGAAATATCGCCATGTTAAAAAACAGACAACGGCAACCGTA
AACTATTACGACATCACTGAACTGTATGAGCAACATCACCCTGAGCTAGACTCGATTATTGAACGCTCGATTCAGTTTTC
TGTGAATCAGCGGGAATTTCAACGCTCAGCAGCCAGTCGTCGTCTACGGGATTTACCCAATATGCAACTGACTTTGGAGC
GTATGGTAAAAAAAGCGGGCATTGACGATGTGGAAACTTTTATGAGCCTCGGTGCGCCAGAAGTGTTTAATAAGGTGCGT
CAAGCTTATGGAAGTGATGTCGATGTAAAACTACTGTGGAAGTTTGCGGGTGCGATTGAAGGCATTCACTGGAAACTGCT
GCAAGAGCCGCGTAAGCGCCAACTGTTAGAAAGTTGTCAGCAGCGTTAA