Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00372 experimental
T6SS type Type i4b
Strain Edwardsiella tarda EIB202
Replicon (RefSeq) chromosome (NC_013508)
Location 2555437..2575336
Function anti-eukaryotic; effector translocation; adherence; virulence
Comment Virulence in blue gourami fish; virulence in several other fish models; invasion of cultured carp epithelial cells.
References
[1] Wang X et al (2009) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish Shellfish Immunol. 27(3):469-77. [PudMed:19563898]
[2] Leung KY et al (2012) Edwardsiella tarda - virulence mechanisms of an emerging gastroenteritis pathogen. Microbes Infect. 14(1):26-34. [PudMed:21924375]
[3] Lv Y et al (2012) Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Vet Microbiol. 157(1-2):190-9. [PudMed:22227416]
[4] Jobichen C et al (2010) Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 5(9):e12910. [PudMed:20886112]
[5] Yang M et al (2012) Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLoS One. 7(5):e36987. [PudMed:22590641]
[6] Chakraborty S et al (2011) Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 286(45):39417-30. [PudMed:21953460]
[7] Zheng J et al (2007) Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 66(5):1192-206. [PudMed:17986187]
[8] Zhang J et al (2014) A new target for the old regulator: H-NS suppress T6SS secretory protein EvpP, the major virulence factor in the fish pathogen Edwardsiella tarda. Lett Appl Microbiol. 59(5):557-564. [PudMed:25131176]
[9] Wang X et al (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish Shellfish Immunol. 29(6):1082-91. [PudMed:20832475]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPAARTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number11111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
ETAE_RS11215 2550717..2550998 - 282 WP_012849252.1 YfcL family protein
ETAE_RS11220 2551037..2551585 - 549 WP_012849253.1 elongation factor P hydroxylase
ETAE_RS11225 2551686..2552486 - 801 WP_012849254.1 TSUP family transporter
ETAE_RS11230 2552790..2554244 - 1455 WP_071818527.1 SH3 domain-containing protein
ETAE_RS18215 2554576..2555136 + 561 WP_172454866.1 hypothetical protein
T6CP003806 TssB ETAE_RS11240 (tssB) 2555386..2555901 + 516 WP_015683417.1 type VI secretion system contractile sheath small subunit
T6CP003807 TssC ETAE_RS11245 (tssC) 2555898..2557385 + 1488 WP_012849259.1 type VI secretion system contractile sheath large subunit
T6CP003808 TssD ETAE_RS11250 2557455..2557946 + 492 WP_012849260.1 type VI secretion system tube protein Hcp
ETAE_RS11255 2558046..2559248 + 1203 WP_012849261.1 hypothetical protein
T6CP003809 TssE ETAE_RS11260 (tssE) 2559257..2559733 + 477 WP_015461951.1 type VI secretion system baseplate subunit TssE
T6CP003810 TssF ETAE_RS11265 (tssF) 2559737..2561578 + 1842 WP_012849263.1 type VI secretion system baseplate subunit TssF
T6CP003811 TssG ETAE_RS11270 (tssG) 2561575..2562600 + 1026 WP_012849264.1 type VI secretion system baseplate subunit TssG
T6CP003812 TssH ETAE_RS11275 (tssH) 2562752..2565331 + 2580 WP_034168298.1 type VI secretion system ATPase TssH
T6CP003813 TssI ETAE_RS11280 2565331..2567316 + 1986 WP_015461955.1 type VI secretion system tip protein VgrG
T6CP011796 PAAR ETAE_RS11285 2567411..2567713 + 303 WP_012849267.1 PAAR domain-containing protein
T6CP003814 TssA ETAE_RS11290 (tssA) 2567727..2568794 + 1068 WP_012849268.1 type VI secretion system protein TssA
T6CP009342 TssJ ETAE_RS11295 2568781..2569422 + 642 WP_012849269.1 type VI secretion lipoprotein TssJ
T6CP003815 TssK ETAE_RS11300 (tssK) 2569522..2570910 + 1389 WP_012849270.1 type VI secretion system baseplate subunit TssK
T6CP003816 TssL ETAE_RS11305 2570907..2571557 + 651 WP_012849271.1 DotU family type IV/VI secretion system protein
T6CP003817 TssM ETAE_RS11310 2571554..2575336 + 3783 WP_012849272.1 type VI secretion system protein ImpL
ETAE_RS11315 (mepA) 2575468..2576286 - 819 WP_034168301.1 penicillin-insensitive murein endopeptidase
ETAE_RS11320 (aroC) 2576312..2577397 - 1086 WP_012849274.1 chorismate synthase
ETAE_RS11325 (prmB) 2577489..2578421 - 933 WP_012849275.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase
ETAE_RS11330 (smrB) 2578632..2579177 + 546 WP_041692530.1 endonuclease SmrB
ETAE_RS11335 (sixA) 2579228..2579704 - 477 WP_012849277.1 phosphohistidine phosphatase SixA
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0206 experimental ETAE_2873 (RpoS) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3025094..3026080 (-) 502612732 ETAE_0886 (esrB) promoter (↑)
REG0207 experimental ETAE_0035 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 35881..37008 (+) 502609898 ETAE_0886 (esrB) promoter (↑)
REG0208 experimental ETAE_0154 (hemB) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 159867..160889 (+) 502610017 ETAE_0886 (esrB) promoter (↑)
REG0209 experimental ETAE_0743 (cdsA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 820052..820909 (+) 502610607 ETAE_0886 (esrB) promoter (↑)
REG0210 experimental ETAE_2082 (pyrF) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2196136..2196858 (-) 502611934 ETAE_0886 (esrB) promoter (↑)
REG0211 experimental ETAE_1096 (dapA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1194778..1195659 (+) 502610962 ETAE_0886 (esrB) promoter (↑)
REG0212 experimental ETAE_2697 (cysS) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2831230..2832615 (-) 502612557 ETAE_0886 (esrB) promoter (↑)
REG0213 experimental ETAE_1760 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (CP001135) 1870377..1870535 (+) 267984768 ETAE_0886 (esrB) promoter (↑)
REG0214 experimental ETAE_1837 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (CP001135) 1946159..1946284 (-) 267984845 ETAE_0886 (esrB) promoter (↑)
REG0215 experimental ETAE_1202 (ugd) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1306603..1307769 (+) 502611067 ETAE_0886 (esrB) promoter (↑)
REG0216 experimental ETAE_2640 (holA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2779832..2780866 (+) 502612494 ETAE_0886 (esrB) promoter (↑)
REG0217 experimental ETAE_1439 (ruvA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1550776..1551384 (+) 502611302 ETAE_0886 (esrB) promoter (↑)
REG0218 experimental ETAE_0358 (purA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 403538..404836 (+) 502610221 ETAE_0886 (esrB) promoter (↑)
REG0219 experimental ETAE_2813 (iscA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2952011..2952334 (-) 491431780 ETAE_0886 (esrB) promoter (↑)
REG0220 experimental ETAE_2787 (guaB) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2925310..2926776 (-) 502612646 ETAE_0886 (esrB) promoter (↑)
REG0221 experimental ETAE_1412 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1522924..1523409 (-) 502611274 ETAE_0886 (esrB) promoter (↑)
REG0222 experimental ETAE_0430 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 473075..473755 (-) 502610291 ETAE_0886 (esrB) promoter (↑)
REG0223 experimental ETAE_0659 (aceE) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 731608..734271 (+) 502610523 ETAE_0886 (esrB) promoter (↑)
REG0224 experimental ETAE_2773 (mtnN) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2910743..2911441 (+) 502612632 ETAE_0886 (esrB) promoter (↑)
REG0225 experimental ETAE_2468 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2598484..2599356 (-) 504339400 ETAE_0886 (esrB) promoter (↑)
REG0226 experimental ETAE_1614 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1721332..1722024 (-) 754045576 ETAE_0886 (esrB) promoter (↑)
REG0227 experimental ETAE_0994 (Lon) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1085063..1087288 (+) 267984010 ETAE_0886 (esrB) promoter (↓)
REG0228 experimental ETAE_3542 (phoU) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3747537..3748268 (+) 502613380 ETAE_0886 (esrB) promoter (↓)
REG0229 experimental ETAE_3449 (tpiA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3637381..3638145 (+) 502613286 ETAE_0886 (esrB) promoter (↓)
REG0230 experimental ETAE_1655 (prc) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1757898..1759940 (+) 735677771 ETAE_0886 (esrB) promoter (↓)
REG0231 experimental ETAE_1158 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1257412..1258584 (+) 502611022 ETAE_0886 (esrB) promoter (↓)
REG0232 experimental ETAE_0772 (tadA) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 850367..850879 (+) 505698653 ETAE_0886 (esrB) promoter (↓)
REG0233 experimental ETAE_0695 (mrcB) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 766885..769368 (+) 502610560 ETAE_0886 (esrB) promoter (↓)
REG0234 experimental ETAE_1170 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (CP001135) 1271304..1271447 (-) 267984186 ETAE_0886 (esrB) promoter (↓)
REG0235 experimental ETAE_1562 protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1668753..1670378 (-) 780104675 ETAE_0886 (esrB) promoter (↓)
REG0236 experimental ETAE_3453 (cpxR) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3641204..3641902 (+) 502613290 ETAE_0886 (esrB) promoter (↓)
REG0237 experimental ETAE_0263 (mltC) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 293215..294306 (-) 1392602598 ETAE_0886 (esrB) promoter (↓)
REG0238 experimental ETAE_0542 (slt) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 588958..590886 (+) 502610404 ETAE_0886 (esrB) promoter (↓)
REG0239 experimental ETAE_2638 (leuS) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 2776570..2779152 (+) 502612492 ETAE_0886 (esrB) promoter (↓)
REG0240 experimental ETAE_3207 (rplQ) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3390207..3390593 (-) 491439763 ETAE_0886 (esrB) promoter (↓)
REG0241 experimental ETAE_1010 (acrB) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1099309..1102461 (-) 502610875 ETAE_0886 (esrB) promoter (↓)
REG0242 experimental ETAE_1425 (mviN) protein TCS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 1535368..1536906 (+) 502611288 ETAE_0886 (esrB) promoter (↓)
REG0243 experimental ETAE_0886 (EsrB) protein TCS-RR Edwardsiella tarda EIB202 chromosome (NC_013508) 964658..965302 (-) 502610749 Unknown (↓); Unknown (↓)
REG0244 experimental ETAE_2059 (phoP) protein TCS-HK Edwardsiella tarda EIB202 chromosome (NC_013508) 2172565..2173239 (+) 502611910 ETAE_0886 (EsrB) (↓)
REG0245 experimental ETAE_3098 (PepA) protein Transcription regulator Edwardsiella tarda EIB202 chromosome (NC_013508) 3260322..3261833 (+) 502612947 ETAE_0886 (esrB) promoter (↑)
REG0246 experimental ETAE_3474 (fabR) protein QS regulatory pathway Edwardsiella tarda EIB202 chromosome (NC_013508) 3666488..3667159 (-) 502613311 Unknown (↓)
REG0247 experimental ETAE_1505 (H-NS) protein Transcription regulator Edwardsiella tarda EIB202 chromosome (NC_013508) 1615099..1615506 (-) 505274277 ETAE_2430 (evpB) (↑); evpC promotor (↑)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01477 experimental ETAE_1303 (EseL) Tse - Edwardsiella tarda EIB202 chromosome (NC_013508) 1411812..1412027 (-) 502611166
EFF01478 experimental ETAE_2136 (EseM) Tse - Edwardsiella tarda EIB202 chromosome (NC_013508) 2248536..2248751 (+) 502611987
EFF00478 experimental ETAE_RS18215 Tse - Edwardsiella tarda EIB202 chromosome (NC_013508) 2554576..2555136 (+) 269139773
EFF00479 experimental ETAE_RS11250 - - Edwardsiella tarda EIB202 chromosome (NC_013508) 2557455..2557946 (+) 269139776

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector