Detailed information of regulator


Summary

Regulator ID REG0244 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Cationic antimicrobial peptide (CAMP) resistance (map01503)
Related T6SS T6SS00372 experimental (Type i4b)
Strain Edwardsiella tarda EIB202
Replicon chromosome
Sequence Protein sequence (225 a.a.); Nucleotide sequence (675 bp)
Description The mutation of phoP exhibited a slight but reproducible and significant decrease of ECPs secretion when compared to the wild-type strain.When pDM8G-esrB was introduced into the phoP mutant, the b-galactosidase activity was reduced 1.6-fold compared to that of the wild type. Deletion of phoP also reduced the activity of esrA-lacZ fusion by 1.4-fold.
Reference
External database links

Locus tag (Gene) ETAE_2059 (phoP)
Coordinates (Strand) 2172565..2173239 (+)
NCBI ID 502611910
RefSeq NC_013508
Uniprot ID D0Z9B8_EDWTE, A0A0H3DVI7_EDWTF
KEGG ID etd:ETAF_1858, etr:ETAE_2059
KEGG pathway (determined by BlastKOALA) map02020; map01503
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
ETAE_0886 (EsrB) ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 9.2e-24 3..112
Trans_reg_C PF00486.30 2.2e-19 148..220
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-224 MRILVVEDNNLLRHHLSVQMRELGHQVDAAENAKEADFFVREQSPDIAIVDLGLPGEDGMSMIRRWRSEQITLPILVLTARESWQEKVAVLEAGADDYVTKPFHLEEVLARMQALMRRNSGLASQIISMPPFQADLSRRELRVNETLIKLTAFEYTIIETLIRNSGKVVSKDALMLQLYPDAELRESHTIDVLMGRLRKKLQNEYPGDVITTVRGQGYRFDVPA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9941 - -


  Protein sequence of regulator: 225 a.a.    .

>REG0244 NC_013508:2172565-2173239 [Edwardsiella tarda EIB202]
MRILVVEDNNLLRHHLSVQMRELGHQVDAAENAKEADFFVREQSPDIAIVDLGLPGEDGMSMIRRWRSEQITLPILVLTA
RESWQEKVAVLEAGADDYVTKPFHLEEVLARMQALMRRNSGLASQIISMPPFQADLSRRELRVNETLIKLTAFEYTIIET
LIRNSGKVVSKDALMLQLYPDAELRESHTIDVLMGRLRKKLQNEYPGDVITTVRGQGYRFDVPA*

  Nucleotide sequence of regulator: 675 bp    .

>REG0244 NC_013508:2172565-2173239 [Edwardsiella tarda EIB202]
ATGCGTATCTTAGTCGTCGAAGACAACAATCTGCTGCGCCATCACCTGTCGGTACAAATGCGCGAGCTGGGCCACCAGGT
CGATGCCGCGGAAAACGCCAAAGAGGCGGACTTTTTTGTCCGCGAACAGAGCCCTGATATCGCGATCGTCGACCTCGGCC
TGCCGGGAGAAGACGGCATGAGCATGATCCGCCGCTGGCGCAGCGAGCAGATCACCCTGCCCATTCTGGTGCTCACCGCC
CGGGAGAGCTGGCAGGAAAAGGTGGCGGTGCTGGAGGCGGGCGCCGATGACTATGTCACCAAACCCTTCCATTTAGAAGA
GGTGCTGGCGCGCATGCAGGCGCTGATGCGCCGCAACAGCGGCCTGGCCTCACAGATCATCAGCATGCCCCCTTTCCAGG
CGGATCTCTCTCGCCGCGAGCTGCGGGTCAACGAAACGCTGATCAAGCTCACCGCTTTCGAGTACACCATCATAGAGACC
CTGATCCGCAACAGCGGCAAAGTGGTCAGCAAAGATGCCCTGATGCTGCAGCTCTATCCCGACGCCGAGCTGCGCGAAAG
CCATACCATCGACGTACTGATGGGACGCCTGCGCAAAAAGCTGCAGAATGAGTACCCGGGCGACGTCATCACCACCGTGC
GCGGTCAGGGCTACCGCTTCGACGTGCCGGCATGA