Detailed information of effector
Summary
| Effector ID | EFF00478  |
| Effector type | Tse |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (187 a.a.); Nucleotide sequence (561 bp) |
| Description | EvpP inhibits neutrophils recruitment through Jnk-caspy-inflammasome cascades in vivo. |
| Reference | [1] Zheng J, Leung KY. Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 2007 Dec;66(5):1192-206. Epub 2007 Nov 6. PMID: 17986187 [2] Chen H, Yang D, Han F, et al. The Bacterial T6SS Effector EvpP Prevents NLRP3 Inflammasome Activation by Inhibiting the Ca2+-Dependent MAPK-Jnk Pathway. Cell Host Microbe. 2017 Jan 11;21(1):47-58. doi: 10.1016/j.chom.2016.12.004. PMID: 28081443 [3] Tan J, Yang D, Wang Z, et al. EvpP inhibits neutrophils recruitment via Jnk-caspy inflammasome signaling in vivo. Fish Shellfish Immunol. 2019 Sep;92:851-860. doi: 10.1016/j.fsi.2019.05.051. Epub 2019 May 23. PMID: 31129187 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ETAE_RS18215 |
| Coordinates (Strand) | 2554576..2555136 (+) |
| NCBI ID | 269139773 |
| RefSeq | NC_013508 |
| Uniprot ID | UPI0001BD08F7 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-186 | MTLNSELEWSDVGSICTDQKAKTYVSDAFNIAYGQPTKELLPAGTALYKFNGFSSLARSPITDDSPLSPWWSPVQPFRYDGGLRQRMLIAKQNGVSMREWGRLTSVIKENWSSLDFLLEIVLKIPVYAWFGGFKGMSRIDNGMTSKRNITLEQKGRSSMLPGGATQFYIPNLTVGHISSHQFSILK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9802 | - | - |
Protein sequence: 187 a.a. .
>EFF00478 NC_013508:2554576-2555136 Type VI Secretion System Effector (Tse) [Edwardsiella tarda EIB202]
MTLNSELEWSDVGSICTDQKAKTYVSDAFNIAYGQPTKELLPAGTALYKFNGFSSLARSPITDDSPLSPWWSPVQPFRYD
GGLRQRMLIAKQNGVSMREWGRLTSVIKENWSSLDFLLEIVLKIPVYAWFGGFKGMSRIDNGMTSKRNITLEQKGRSSML
PGGATQFYIPNLTVGHISSHQFSILK*
MTLNSELEWSDVGSICTDQKAKTYVSDAFNIAYGQPTKELLPAGTALYKFNGFSSLARSPITDDSPLSPWWSPVQPFRYD
GGLRQRMLIAKQNGVSMREWGRLTSVIKENWSSLDFLLEIVLKIPVYAWFGGFKGMSRIDNGMTSKRNITLEQKGRSSML
PGGATQFYIPNLTVGHISSHQFSILK*
Nucleotide sequence: 561 bp .
>EFF00478 NC_013508:2554576-2555136 Type VI Secretion System Effector (Tse) [Edwardsiella tarda EIB202]
TTGACTTTGAATTCCGAGCTGGAATGGTCCGATGTTGGAAGCATTTGTACAGATCAAAAGGCAAAAACCTACGTCAGCGA
TGCATTTAACATAGCCTATGGGCAGCCGACGAAAGAGCTGCTCCCGGCGGGAACAGCCTTATACAAATTCAATGGATTTA
GTTCATTGGCAAGGTCCCCGATCACTGACGATAGCCCGCTATCTCCCTGGTGGAGCCCGGTCCAGCCATTCCGGTACGAT
GGCGGTCTGCGCCAGCGTATGCTGATCGCTAAGCAAAACGGGGTATCAATGAGGGAATGGGGACGACTCACCTCCGTGAT
CAAAGAAAACTGGAGCTCTCTCGACTTTTTACTTGAGATAGTCCTAAAGATTCCCGTCTATGCCTGGTTCGGTGGATTTA
AGGGCATGTCGCGCATTGATAATGGTATGACCTCTAAACGAAATATCACGCTGGAACAAAAAGGCCGGAGCTCAATGCTC
CCCGGCGGGGCCACACAGTTCTATATTCCAAACCTGACGGTCGGGCATATCAGTAGCCACCAATTTTCAATATTGAAATA
G
TTGACTTTGAATTCCGAGCTGGAATGGTCCGATGTTGGAAGCATTTGTACAGATCAAAAGGCAAAAACCTACGTCAGCGA
TGCATTTAACATAGCCTATGGGCAGCCGACGAAAGAGCTGCTCCCGGCGGGAACAGCCTTATACAAATTCAATGGATTTA
GTTCATTGGCAAGGTCCCCGATCACTGACGATAGCCCGCTATCTCCCTGGTGGAGCCCGGTCCAGCCATTCCGGTACGAT
GGCGGTCTGCGCCAGCGTATGCTGATCGCTAAGCAAAACGGGGTATCAATGAGGGAATGGGGACGACTCACCTCCGTGAT
CAAAGAAAACTGGAGCTCTCTCGACTTTTTACTTGAGATAGTCCTAAAGATTCCCGTCTATGCCTGGTTCGGTGGATTTA
AGGGCATGTCGCGCATTGATAATGGTATGACCTCTAAACGAAATATCACGCTGGAACAAAAAGGCCGGAGCTCAATGCTC
CCCGGCGGGGCCACACAGTTCTATATTCCAAACCTGACGGTCGGGCATATCAGTAGCCACCAATTTTCAATATTGAAATA
G