Detailed information of regulator


Summary

Regulator ID REG0233 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Peptidoglycan biosynthesis (map00550)
Related T6SS T6SS00372 experimental (Type i4b)
Strain Edwardsiella tarda EIB202
Replicon chromosome
Sequence Protein sequence (828 a.a.); Nucleotide sequence (2484 bp)
Description Putative activators of esrB as identified by Tn-seq.
Reference
External database links

Locus tag (Gene) ETAE_0695 (mrcB)
Coordinates (Strand) 766885..769368 (+)
NCBI ID 502610560
RefSeq NC_013508
Uniprot ID D0ZCR1_EDWTE, A0A0H3DQL4_EDWTF
KEGG ID etr:ETAE_0695, etd:ETAF_0637
KEGG pathway (determined by BlastKOALA) map01100; map00550
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
ETAE_0886 (esrB) promoter ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Transgly PF00912.24 2.4e-54 217..387
UB2H PF14814.8 9.2e-26 121..205
Transpeptidase PF00905.24 5.7e-23 481..721
PBP1_TM PF14812.8 3.8e-21 1..87
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-72 MSGDDRQPIGRKGKKRAAQPPRGEPARRRRRYDEDDYDEDDQAREESDDDGYDDDDEGDVARKQVKRSRRRR
Transmembrane helix 73-95 VLWLLFRLALVALIVIGAYGVYL
Outside 96-827 DQQIRSRIDGKVWQLPAAVYGRMVSLEPGMNYSKKEMVNLLEGMQYREVSRITRPGEFTVSGSSIDMLRRPFDFPDGKEGQIHARLVFDADGLSEIVNLENKRQFGFFRLDPRLITMLQSPNGEQRLFVPRNGFPTLLVDTLLATEDRHFYQHDGISFYSIGRALLANLTAGRTVQGGSTLTQQLVKNLFLSNERSLWRKLNEAYMALILDYRYSKDRILELYLNEVYLGQSGNDQIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASLYNPWRNPKLALERRNVVLHLLLEQKIIDQDLYNMLSARPLGVQPKGGVISPQPAFMQLVRQELQAKLGDKVQDLSGVKIFTTLDPVSQDAAEKAVEAGIPALRAARHLDDLEAAMVVVDRFSGEVRAMVGGAQTQFAGFNRAMDARRLIGSLAKPPTYLTALSEPVSYRLNTPLTDQPVSIKLSNGTYWEPKNYDRQYRGQVMLMTALAQSLNVPTVNLGMALGLDKISATWQQLGVPKSVIDPVPAMLLGAINLTPMEVAQAYQTIAAGGNRATLSALRSVIAEDGTVLYQSLPQAQSVVAPQAAYLTLYAMQQGVVSGTSRSLSGKFGRYHLAAKTGTTNDLRDSWFAGIDGKEVAITWVGRDNNGPSKLTGASGALTLYRRYLENQTPLALDLVPPQGISMVGIDDAGNFQCGSGRTLPVWTDDPQSLCSAPPPAAAGQGQGSDGVASWIKDMFGRN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9471 - -


  Protein sequence of regulator: 828 a.a.    .

>REG0233 NC_013508:766885-769368 [Edwardsiella tarda EIB202]
MSGDDRQPIGRKGKKRAAQPPRGEPARRRRRYDEDDYDEDDQAREESDDDGYDDDDEGDVARKQVKRSRRRRVLWLLFRL
ALVALIVIGAYGVYLDQQIRSRIDGKVWQLPAAVYGRMVSLEPGMNYSKKEMVNLLEGMQYREVSRITRPGEFTVSGSSI
DMLRRPFDFPDGKEGQIHARLVFDADGLSEIVNLENKRQFGFFRLDPRLITMLQSPNGEQRLFVPRNGFPTLLVDTLLAT
EDRHFYQHDGISFYSIGRALLANLTAGRTVQGGSTLTQQLVKNLFLSNERSLWRKLNEAYMALILDYRYSKDRILELYLN
EVYLGQSGNDQIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASLYNPWRNPKLALERRNVVLHLLLEQKIIDQDLYN
MLSARPLGVQPKGGVISPQPAFMQLVRQELQAKLGDKVQDLSGVKIFTTLDPVSQDAAEKAVEAGIPALRAARHLDDLEA
AMVVVDRFSGEVRAMVGGAQTQFAGFNRAMDARRLIGSLAKPPTYLTALSEPVSYRLNTPLTDQPVSIKLSNGTYWEPKN
YDRQYRGQVMLMTALAQSLNVPTVNLGMALGLDKISATWQQLGVPKSVIDPVPAMLLGAINLTPMEVAQAYQTIAAGGNR
ATLSALRSVIAEDGTVLYQSLPQAQSVVAPQAAYLTLYAMQQGVVSGTSRSLSGKFGRYHLAAKTGTTNDLRDSWFAGID
GKEVAITWVGRDNNGPSKLTGASGALTLYRRYLENQTPLALDLVPPQGISMVGIDDAGNFQCGSGRTLPVWTDDPQSLCS
APPPAAAGQGQGSDGVASWIKDMFGRN*

  Nucleotide sequence of regulator: 2484 bp    .

>REG0233 NC_013508:766885-769368 [Edwardsiella tarda EIB202]
ATGTCTGGGGACGATCGCCAGCCAATCGGGCGTAAAGGTAAAAAACGGGCGGCGCAGCCGCCACGCGGAGAGCCTGCGCG
CCGCCGCCGTCGTTATGACGAGGATGACTACGACGAGGATGACCAGGCGCGTGAGGAGAGTGATGATGACGGATATGATG
ACGATGATGAGGGTGATGTGGCGCGCAAACAGGTAAAACGTTCCCGTCGGCGCCGCGTGCTGTGGCTGCTGTTCCGTCTG
GCGCTGGTCGCGCTGATCGTCATCGGCGCCTATGGGGTCTATCTCGATCAGCAGATCCGCAGCCGTATCGACGGTAAAGT
CTGGCAGCTGCCGGCCGCCGTGTATGGCCGGATGGTCAGCCTTGAGCCGGGGATGAACTACAGCAAAAAAGAGATGGTCA
ATCTGCTGGAGGGCATGCAGTACCGCGAGGTGAGCCGCATCACCCGTCCCGGCGAGTTTACCGTTTCGGGCAGCAGTATC
GATATGCTGCGTCGTCCCTTTGATTTTCCCGATGGAAAAGAGGGGCAGATCCACGCTCGCCTGGTGTTTGACGCGGATGG
CCTGAGCGAAATTGTCAATCTGGAGAATAAGCGCCAGTTTGGCTTTTTCCGCCTCGATCCCCGGCTGATCACGATGCTGC
AGTCGCCCAACGGCGAGCAGCGTCTGTTTGTGCCGCGCAACGGCTTCCCGACGCTGCTGGTGGATACCCTGCTGGCGACG
GAGGATCGCCACTTCTATCAGCATGACGGCATCAGCTTCTACTCCATTGGTCGTGCGCTGCTGGCCAACCTGACGGCCGG
CCGTACGGTGCAGGGGGGCAGTACCCTGACCCAGCAGCTGGTGAAAAACCTGTTCCTGAGCAATGAGCGCTCCCTGTGGC
GCAAGCTTAACGAAGCCTATATGGCGCTGATCCTCGACTATCGCTACAGCAAGGATCGCATTCTGGAACTGTACCTCAAC
GAGGTGTACCTGGGGCAGAGCGGTAACGATCAGATCCGCGGTTTCCCGCTGGCCAGCCTGTATTACTTTGGCCGCCCGGT
AGAGGAGCTGAGCCTGGATCAGCAGGCGCTGCTGGTGGGCATGGTCAAGGGGGCATCGCTGTATAACCCGTGGCGCAATC
CCAAGCTGGCGCTGGAGCGGCGCAATGTGGTGCTGCACCTGCTGCTGGAGCAGAAGATCATCGATCAGGATCTGTACAAC
ATGCTCAGCGCCCGTCCGCTCGGGGTTCAGCCCAAGGGGGGCGTCATCTCGCCTCAGCCTGCGTTTATGCAGCTGGTGCG
TCAGGAGCTGCAGGCTAAGCTGGGCGATAAGGTACAGGACCTCTCCGGGGTGAAGATCTTTACCACCCTCGATCCTGTCT
CGCAGGATGCGGCGGAAAAAGCCGTCGAGGCCGGTATTCCGGCGCTGCGCGCTGCGCGCCACCTCGACGATCTGGAGGCC
GCCATGGTGGTGGTCGATCGCTTTAGCGGCGAGGTGCGTGCGATGGTGGGCGGCGCGCAGACCCAGTTTGCCGGCTTTAA
CCGCGCGATGGACGCCCGTCGTCTGATCGGCTCGCTGGCCAAGCCGCCGACCTATCTGACGGCGCTGAGTGAGCCGGTGA
GCTACCGTCTGAATACCCCGCTGACGGATCAGCCGGTGTCGATCAAGCTCTCCAACGGCACCTACTGGGAGCCGAAGAAC
TACGATCGTCAGTACCGTGGGCAGGTGATGCTGATGACCGCGCTGGCGCAGTCGCTCAACGTACCGACGGTGAATTTGGG
CATGGCGCTGGGGCTGGATAAGATCAGTGCGACCTGGCAACAGCTGGGCGTCCCGAAAAGCGTCATTGATCCGGTTCCCG
CCATGCTGCTGGGCGCGATTAACCTGACGCCGATGGAGGTGGCTCAGGCCTATCAGACCATCGCCGCCGGCGGTAATCGG
GCGACGCTGTCGGCGCTGCGTTCGGTGATCGCCGAGGACGGCACCGTACTGTATCAGAGCCTGCCCCAGGCGCAGAGCGT
GGTGGCTCCGCAGGCAGCATACCTGACGCTGTATGCCATGCAGCAGGGGGTGGTGAGTGGAACCTCGCGGTCGCTGTCGG
GTAAGTTTGGGCGCTACCATCTGGCGGCCAAGACCGGTACCACGAACGATCTGCGCGACAGCTGGTTTGCCGGCATTGAC
GGGAAGGAGGTCGCGATCACCTGGGTTGGGCGCGATAATAACGGCCCCTCCAAGTTGACCGGGGCCAGCGGTGCCCTGAC
TCTGTACCGCCGTTATCTGGAGAATCAGACGCCGCTGGCGCTGGATCTGGTGCCGCCGCAGGGGATCAGCATGGTAGGGA
TCGATGACGCCGGTAATTTCCAGTGCGGCAGCGGGCGTACCCTGCCGGTCTGGACCGACGATCCGCAGTCGCTGTGCAGT
GCGCCGCCGCCGGCTGCCGCTGGCCAGGGGCAGGGCAGCGACGGTGTCGCCAGCTGGATCAAGGATATGTTTGGTCGTAA
CTGA