Detailed information of regulator
Summary
| Regulator ID | REG0243  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS-RR |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020) |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (215 a.a.); Nucleotide sequence (645 bp) |
| Description | - |
| Reference | [1] Wang X, Wang Q, Xiao J, et al. Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish Shellfish Immunol. 2009 Sep;27(3):469-77. doi: 10.1016/j.fsi.2009.06.013. Epub 2009 Jun 27. PMID: 19563898 [2] Yin K, Peng Y, Ahmed MAH, et al. PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida. Microbiol Res. 2020 Feb;232:126349. doi: 10.1016/j.micres.2019.126349. Epub 2019 Oct 9. PMID: 31816594 |
External database links
| Locus tag (Gene) | ETAE_0886 (EsrB) |
| Coordinates (Strand) | 964658..965302 (-) |
| NCBI ID | 502610749 |
| RefSeq | NC_013508 |
| Uniprot ID | A0A034T5T1_9GAMM, D0ZDM6_EDWTE, Q0VHI7_EDWTA, A0A076LR92_9GAMM, A0A0H3DNV3_EDWTF |
| KEGG ID | etd:ETAF_0830, etr:ETAE_0886, ete:ETEE_2769 |
| KEGG pathway (determined by BlastKOALA) | map02020 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Unknown | ↓ | EvpP expression |
| Unknown | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Response_reg | PF00072.26 | 3.3e-27 | 10..121 |
| GerE | PF00196.21 | 7.8e-19 | 151..205 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-214 | MTISILPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWSDMRIVVVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQILKLVAEGKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9682 | - | - |
Protein sequence of regulator: 215 a.a. .
>REG0243 NC_013508:c965302-964658 [Edwardsiella tarda EIB202]
MTISILPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWS
DMRIVVVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQIL
KLVAEGKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF*
MTISILPLNILIVEDHALLADGIRNLLDSQPRYRVVGAVSNGLAVYEACQRLSPDLVLLDLGLPGMDGVDVIRQLRRRWS
DMRIVVVTASSEARRGSEALAAGALAYVLKQSPQQTLLAAMQHASLGKTFVDPALRLDSLVASSDMAQEPPLTLRERQIL
KLVAEGKRNRDIAELLSISLKTVETHRLNLMRKLDAHNAAELSNWARRLGVLEF*
Nucleotide sequence of regulator: 645 bp .
>REG0243 NC_013508:c965302-964658 [Edwardsiella tarda EIB202]
ATGACTATTTCTATTTTGCCTCTGAACATTCTGATCGTTGAAGATCATGCCTTGCTAGCCGATGGCATCCGTAATCTCTT
GGACAGCCAGCCCCGCTACCGGGTCGTGGGCGCCGTCTCGAATGGGCTGGCCGTTTATGAGGCCTGCCAGCGTCTCTCGC
CCGATCTGGTCTTACTCGATCTGGGTCTGCCGGGCATGGACGGCGTTGACGTGATCCGTCAGCTGCGGCGTCGCTGGAGC
GATATGCGTATCGTCGTCGTCACCGCCTCCTCTGAGGCACGGCGCGGCAGCGAAGCCCTCGCCGCCGGCGCGCTCGCCTA
CGTCCTGAAGCAAAGCCCGCAGCAAACCCTGCTGGCGGCCATGCAGCATGCCTCGCTGGGGAAAACCTTTGTCGATCCCG
CCCTCCGGCTGGACTCCCTGGTAGCCTCGTCCGATATGGCTCAGGAGCCGCCGCTGACCCTGCGCGAACGGCAAATTCTC
AAGCTGGTCGCCGAGGGCAAGCGCAACCGGGATATCGCGGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCACCG
GCTCAACCTGATGCGCAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAACTGGGCCCGCCGCCTGGGGGTTCTGGAGT
TTTAA
ATGACTATTTCTATTTTGCCTCTGAACATTCTGATCGTTGAAGATCATGCCTTGCTAGCCGATGGCATCCGTAATCTCTT
GGACAGCCAGCCCCGCTACCGGGTCGTGGGCGCCGTCTCGAATGGGCTGGCCGTTTATGAGGCCTGCCAGCGTCTCTCGC
CCGATCTGGTCTTACTCGATCTGGGTCTGCCGGGCATGGACGGCGTTGACGTGATCCGTCAGCTGCGGCGTCGCTGGAGC
GATATGCGTATCGTCGTCGTCACCGCCTCCTCTGAGGCACGGCGCGGCAGCGAAGCCCTCGCCGCCGGCGCGCTCGCCTA
CGTCCTGAAGCAAAGCCCGCAGCAAACCCTGCTGGCGGCCATGCAGCATGCCTCGCTGGGGAAAACCTTTGTCGATCCCG
CCCTCCGGCTGGACTCCCTGGTAGCCTCGTCCGATATGGCTCAGGAGCCGCCGCTGACCCTGCGCGAACGGCAAATTCTC
AAGCTGGTCGCCGAGGGCAAGCGCAACCGGGATATCGCGGAGCTGCTGTCCATCAGCCTGAAAACCGTGGAGACCCACCG
GCTCAACCTGATGCGCAAGCTGGATGCCCACAACGCGGCGGAGCTGAGCAACTGGGCCCGCCGCCTGGGGGTTCTGGAGT
TTTAA