Detailed information of component protein


Summary

Component ID T6CP003817
Component protein type TssM
T6SS ID (Type) T6SS00372 experimental (Type i4b)
Strain Edwardsiella tarda EIB202
Replicon chromosome
Sequence Protein sequence (1261 a.a.); Nucleotide sequence (3783 bp)
Reference
[1] Yang M, Lv Y, Xiao J, et al. Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLoS One. 2012;7(5):e36987. doi: 10.1371/journal.pone.0036987. Epub 2012 May 10. PMID: 22590641
[2] Lv Y, Xiao J, Liu Q, et al. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Vet Microbiol. 2012 May 25;157(1-2):190-9. doi: 10.1016/j.vetmic.2011.12.018. Epub 2011 Dec 22. PMID: 22227416
[3] Chakraborty S, Sivaraman J, Leung KY, et al. Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 2011 Nov 11;286(45):39417-30. doi: 10.1074/jbc.M111.295188. Epub 2011 Sep 27. PMID: 21953460
[4] Leung KY, Siame BA, Tenkink BJ, et al. Edwardsiella tarda - virulence mechanisms of an emerging gastroenteritis pathogen. Microbes Infect. 2012 Jan;14(1):26-34. doi: 10.1016/j.micinf.2011.08.005. Epub 2011 Sep 1. PMID: 21924375
[5] Jobichen C, Chakraborty S, Li M, et al. Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda. PLoS One. 2010 Sep 23;5(9):e12910. doi: 10.1371/journal.pone.0012910. PMID: 20886112
[6] Wang X, Wang Q, Xiao J, et al. Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish Shellfish Immunol. 2010 Dec;29(6):1082-91. doi: 10.1016/j.fsi.2010.08.025. Epub 2010 Sep 9. PMID: 20832475
[7] Wang X, Wang Q, Xiao J, et al. Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish Shellfish Immunol. 2009 Sep;27(3):469-77. doi: 10.1016/j.fsi.2009.06.013. Epub 2009 Jun 27. PMID: 19563898
[8] Zheng J, Leung KY. Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol. 2007 Dec;66(5):1192-206. Epub 2007 Nov 6. PMID: 17986187
[9] Zhang J, Xiao J, Zhang Y, et al. A new target for the old regulator: H-NS suppress T6SS secretory protein EvpP, the major virulence factor in the fish pathogen Edwardsiella tarda. Lett Appl Microbiol. 2014 Nov;59(5):557-64. doi: 10.1111/lam.12316. Epub 2014 Sep 12. PMID: 25131176
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ETAE_RS11310
Coordinate (Strand) 2571554..2575336 (+)
NCBI ID WP_012849272.1
RefSeq NC_013508
Uniprot ID D0ZB44_EDWTE
KEGG ID etr:ETAE_2443
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 6.1e-52 172..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKRLFK
Transmembrane helix 7-26 WIAIVVLLSVLAMLCWLLTL
Outside 27-29 WQR
Transmembrane helix 30-52 WPAAAAWVLFLAILAGALLTRYL
Inside 53-417 WRKVRAWRTRSLEAEPVEKARAAERHPMTLQRQWKAATTLLKHSQLRRFRHPLQVLPWFMLLGRPGSGKSALLAQSGLAAPLDLPHELNDGKAPVDWWYFDKAVILDISGQFVQPEASQETRSTWNRMLDLLGRDRARAGLNGLVLTVSAQTLLSADGGGVVDEGRLIRTRIEQLIRLFDRRFPVYVLVTQIDSLYGMEAWSHLLTPEALDQAMGHVVDEPENGRDVRDVIDIALGRVNSRLKTMQLTMLLQHPDADPALLMFPTELARLAEPLKRYVYHALGDSPYLESPILRGVFFASAVQKGNTRSGPEDQQEIALQEAAHDTVKRGVFVRDLFNRVLPADRHLNRPVAVTSPWRRLNRHLG
Transmembrane helix 418-440 LFAWWLCVAAALGYLGVSVVYWQ
Outside 441-1260 HTLDDLRQRYPATMAMNRALPHDMQMMIAQRDAIYWLENRNAQWASRVLAFQHQLLSVEADAKSQYVNDFSQYILPSLDKAIQQSTTADSIASHPDGLVNAIQVEVRRANLLRARLRGSSYGALMAMPPIPTPPLRLLYPALDSDTVSRFNDLFLAQLAWSTDIQQQNARLLHLQARLRQLAAQSATLDWIIPWANAQPDLTSVTLDDFWQGSLRPDKLPEVDAAYTLEGQQRIRAFLVEMRSSMPNDIAFDKKRAAFWHEYAQKRLQAWQDFAGQFSQGERILAGQGEWQSTIDRLPHDDNPYLRLARRLLTEFAEMPDDLRPGWLRVLPQWLEIRQRAHQQALVRVPGLVSDVGGMILRGTAQKGALDQTRSQLVGRLDAIRLYQEYQAAMTQAMNSVVISQAQATKVAADFHGFDSQQSGQSSLQTAYARLMSLRSLLGYNRPDEQLTWGLMAGPLQLVIRYADQQASCFLQSAWQDRVIAPSRWTLNQAERADKLYGDNGLLWTFLSGPIKPFIQRNDSGWQEVDTLGQSVPFTADFLPFINRALIRQVDLKAGKIALHQKQQQATLAQQQQKQRWQTVAAESGEKLAELNTAAAALKAKSYPVALTGLPTDVNSNAKAKVVGTLLTMQCTNATLRLNNLNFAVMQSVLWSQQQCGQVTLQIRLPAFTLTRSYPGPQGFVHFLRDFPGGARRFTPADFPVDKSRMAAVGLTQITVRYRIAGQETLLQDAAQLAQAERDIQSAQTRQQQAQQALLTLNRPSASESLPPLDTLPGLTMMSVPERIGQCWRGDIRLARENDGIGRLMSELVAKQLSDKP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9912 - -


  Protein sequence: 1261 a.a.    

>T6CP003817 NC_013508:2571554-2575336 [Edwardsiella tarda EIB202] [TssM]
MKRLFKWIAIVVLLSVLAMLCWLLTLWQRWPAAAAWVLFLAILAGALLTRYLWRKVRAWRTRSLEAEPVEKARAAERHPM
TLQRQWKAATTLLKHSQLRRFRHPLQVLPWFMLLGRPGSGKSALLAQSGLAAPLDLPHELNDGKAPVDWWYFDKAVILDI
SGQFVQPEASQETRSTWNRMLDLLGRDRARAGLNGLVLTVSAQTLLSADGGGVVDEGRLIRTRIEQLIRLFDRRFPVYVL
VTQIDSLYGMEAWSHLLTPEALDQAMGHVVDEPENGRDVRDVIDIALGRVNSRLKTMQLTMLLQHPDADPALLMFPTELA
RLAEPLKRYVYHALGDSPYLESPILRGVFFASAVQKGNTRSGPEDQQEIALQEAAHDTVKRGVFVRDLFNRVLPADRHLN
RPVAVTSPWRRLNRHLGLFAWWLCVAAALGYLGVSVVYWQHTLDDLRQRYPATMAMNRALPHDMQMMIAQRDAIYWLENR
NAQWASRVLAFQHQLLSVEADAKSQYVNDFSQYILPSLDKAIQQSTTADSIASHPDGLVNAIQVEVRRANLLRARLRGSS
YGALMAMPPIPTPPLRLLYPALDSDTVSRFNDLFLAQLAWSTDIQQQNARLLHLQARLRQLAAQSATLDWIIPWANAQPD
LTSVTLDDFWQGSLRPDKLPEVDAAYTLEGQQRIRAFLVEMRSSMPNDIAFDKKRAAFWHEYAQKRLQAWQDFAGQFSQG
ERILAGQGEWQSTIDRLPHDDNPYLRLARRLLTEFAEMPDDLRPGWLRVLPQWLEIRQRAHQQALVRVPGLVSDVGGMIL
RGTAQKGALDQTRSQLVGRLDAIRLYQEYQAAMTQAMNSVVISQAQATKVAADFHGFDSQQSGQSSLQTAYARLMSLRSL
LGYNRPDEQLTWGLMAGPLQLVIRYADQQASCFLQSAWQDRVIAPSRWTLNQAERADKLYGDNGLLWTFLSGPIKPFIQR
NDSGWQEVDTLGQSVPFTADFLPFINRALIRQVDLKAGKIALHQKQQQATLAQQQQKQRWQTVAAESGEKLAELNTAAAA
LKAKSYPVALTGLPTDVNSNAKAKVVGTLLTMQCTNATLRLNNLNFAVMQSVLWSQQQCGQVTLQIRLPAFTLTRSYPGP
QGFVHFLRDFPGGARRFTPADFPVDKSRMAAVGLTQITVRYRIAGQETLLQDAAQLAQAERDIQSAQTRQQQAQQALLTL
NRPSASESLPPLDTLPGLTMMSVPERIGQCWRGDIRLARENDGIGRLMSELVAKQLSDKP*

  Nucleotide sequence: 3783 bp    

>T6CP003817 NC_013508:2571554-2575336 [Edwardsiella tarda EIB202] [TssM]
ATGAAACGTCTCTTCAAATGGATAGCGATCGTCGTGCTCCTGAGCGTACTGGCGATGCTGTGCTGGCTGCTCACGCTGTG
GCAACGCTGGCCCGCCGCCGCCGCATGGGTGCTGTTTCTCGCCATTCTGGCCGGCGCCCTGCTGACCCGCTATCTCTGGC
GTAAGGTGCGGGCATGGCGTACCCGCAGCCTGGAGGCGGAGCCGGTTGAAAAAGCCCGAGCCGCGGAGCGCCATCCCATG
ACGCTACAGCGCCAGTGGAAAGCCGCCACGACCCTGCTGAAGCACTCTCAGCTGCGCCGGTTCCGCCACCCGCTGCAGGT
TCTGCCCTGGTTTATGCTGCTGGGCCGCCCGGGATCGGGAAAATCGGCGCTGCTGGCGCAGTCGGGCCTGGCCGCCCCCT
TGGATTTGCCCCATGAGCTCAATGACGGCAAGGCGCCCGTCGACTGGTGGTATTTCGACAAGGCCGTCATTCTGGATATC
TCCGGCCAGTTCGTACAGCCGGAGGCATCACAGGAGACCCGCTCCACCTGGAACCGGATGCTGGATCTGCTGGGGCGCGA
TCGCGCCCGGGCCGGATTGAACGGGCTGGTGCTCACGGTATCGGCCCAGACCCTGCTGTCCGCAGACGGGGGCGGCGTGG
TCGACGAGGGACGGCTGATCCGCACTCGCATAGAGCAGCTGATCCGTCTGTTCGACAGGCGCTTCCCCGTCTACGTGCTG
GTGACCCAAATCGACAGCCTGTACGGCATGGAGGCCTGGTCCCACCTGCTGACCCCGGAGGCGCTGGATCAGGCCATGGG
CCATGTGGTGGACGAACCGGAGAACGGCCGCGACGTCCGCGACGTGATAGATATCGCCTTGGGTCGGGTCAATAGCCGGC
TGAAAACCATGCAGCTGACGATGCTGCTGCAGCACCCGGATGCCGATCCGGCCTTACTGATGTTCCCCACCGAGCTGGCC
AGGCTGGCCGAGCCCCTCAAGCGCTATGTCTATCATGCCCTGGGAGACAGCCCCTATCTGGAGTCGCCCATCTTGCGCGG
CGTATTCTTCGCCAGCGCGGTCCAGAAGGGAAACACCCGCAGCGGGCCCGAAGATCAGCAGGAGATTGCGCTGCAGGAGG
CGGCGCATGACACCGTCAAACGCGGGGTCTTTGTCCGGGATCTGTTTAACCGCGTCCTGCCGGCCGACCGTCATCTGAAC
CGCCCCGTCGCCGTGACCAGTCCCTGGCGGCGCCTGAACCGCCATCTGGGCCTGTTCGCCTGGTGGCTGTGCGTGGCCGC
CGCCCTCGGGTATCTCGGCGTATCGGTCGTATACTGGCAGCACACGCTGGACGATCTGCGCCAGCGCTATCCGGCGACCA
TGGCCATGAATCGCGCCCTGCCCCACGACATGCAGATGATGATAGCCCAGCGGGATGCGATTTATTGGCTGGAAAACAGA
AACGCGCAGTGGGCCAGCCGGGTACTGGCATTCCAGCATCAGTTGCTCTCGGTTGAAGCGGATGCAAAATCGCAGTATGT
AAATGACTTTAGCCAATATATTTTACCCAGCCTGGACAAGGCGATTCAGCAGAGCACCACGGCGGACAGCATCGCATCCC
ATCCCGACGGGCTGGTGAACGCCATTCAGGTGGAGGTCCGCCGCGCCAACCTGCTACGGGCGAGGCTGCGAGGCTCCAGC
TACGGAGCGCTGATGGCCATGCCCCCGATACCAACGCCCCCCCTGCGGTTACTGTACCCGGCGCTGGATTCGGATACGGT
ATCCCGTTTCAATGACCTGTTCCTGGCCCAGCTGGCCTGGTCAACGGATATCCAGCAGCAGAACGCCCGCCTGCTGCACC
TGCAGGCGCGGCTCCGGCAGTTGGCGGCGCAAAGCGCCACCCTGGACTGGATCATTCCCTGGGCAAACGCCCAGCCGGAT
CTGACCAGCGTGACCCTGGACGACTTCTGGCAGGGCTCACTGCGGCCGGATAAATTGCCGGAGGTCGATGCGGCCTACAC
CCTGGAGGGGCAGCAGCGTATCCGCGCATTCCTGGTGGAGATGCGCAGCAGCATGCCGAACGACATCGCCTTCGACAAAA
AACGGGCCGCCTTCTGGCATGAATATGCGCAGAAGCGCTTGCAGGCCTGGCAGGACTTCGCCGGACAGTTCTCTCAGGGA
GAGCGGATCCTGGCAGGTCAGGGAGAGTGGCAGAGCACCATTGACAGACTGCCCCACGATGACAACCCGTATCTGCGCCT
TGCCCGCCGCCTGTTGACCGAGTTTGCCGAGATGCCGGATGACCTGCGCCCGGGCTGGCTCCGGGTGCTCCCGCAGTGGC
TGGAGATACGCCAGCGCGCCCACCAGCAGGCCCTCGTCAGGGTCCCCGGACTGGTCAGCGATGTGGGCGGCATGATCCTG
CGCGGGACGGCGCAGAAGGGGGCGCTGGATCAGACCCGCAGCCAGCTCGTCGGCCGGCTGGATGCCATCCGCCTGTATCA
GGAGTATCAGGCGGCCATGACCCAGGCCATGAACAGCGTCGTCATCAGCCAGGCCCAGGCCACCAAAGTGGCGGCGGACT
TCCATGGCTTTGACAGCCAGCAGAGCGGGCAGTCCTCACTGCAGACGGCCTATGCACGCCTGATGAGCCTGCGCAGCCTG
CTGGGATACAATCGGCCCGATGAACAGCTCACCTGGGGGCTGATGGCGGGCCCCTTGCAGCTGGTCATTCGCTATGCCGA
TCAGCAGGCCTCCTGCTTCCTGCAGAGCGCGTGGCAGGACCGCGTCATCGCGCCATCGCGCTGGACCCTCAATCAGGCCG
AGCGGGCGGATAAGCTGTACGGTGACAATGGGTTGCTCTGGACCTTTTTATCCGGGCCGATTAAGCCGTTTATCCAACGT
AACGATAGCGGCTGGCAGGAGGTGGATACGCTGGGGCAGTCCGTCCCCTTTACCGCAGACTTTCTGCCATTCATCAACCG
GGCCCTTATCCGCCAGGTTGATCTGAAGGCCGGGAAAATCGCGCTACACCAGAAACAGCAGCAGGCCACCCTGGCCCAGC
AGCAGCAAAAGCAGCGCTGGCAAACCGTGGCCGCCGAGTCTGGCGAAAAGCTGGCCGAGCTGAACACCGCCGCCGCCGCC
CTGAAGGCGAAAAGCTATCCCGTCGCCCTGACGGGGCTGCCGACGGACGTCAACAGCAACGCCAAGGCCAAGGTCGTGGG
AACCCTGCTGACCATGCAGTGTACCAATGCCACGCTACGGCTAAACAACCTTAACTTTGCCGTGATGCAAAGCGTACTCT
GGTCACAGCAGCAGTGCGGCCAGGTGACGCTGCAGATCCGGCTCCCGGCCTTCACGCTGACGCGCAGCTATCCCGGGCCT
CAGGGCTTTGTCCACTTTTTACGGGATTTCCCGGGCGGGGCCCGCCGGTTTACCCCGGCGGACTTCCCCGTCGACAAAAG
TCGGATGGCGGCGGTCGGGCTAACCCAGATTACGGTACGCTACCGCATCGCCGGACAGGAGACGCTGCTGCAGGACGCCG
CTCAGCTGGCGCAGGCCGAGCGCGATATTCAGTCAGCCCAGACCCGCCAACAGCAGGCGCAGCAGGCCCTGCTGACGCTG
AATAGGCCGAGCGCATCGGAGAGCCTTCCGCCGCTGGATACGCTGCCGGGGCTGACGATGATGAGTGTGCCGGAGCGCAT
CGGGCAGTGCTGGCGGGGGGATATTCGCCTGGCCAGAGAAAACGACGGCATTGGCCGGCTGATGAGTGAACTGGTCGCGA
AACAGTTAAGCGACAAGCCGTAA