Detailed information of regulator
Summary
| Regulator ID | REG0231  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (391 a.a.); Nucleotide sequence (1173 bp) |
| Description | Putative activators of esrB as identified by Tn-seq. |
| Reference |
External database links
| Locus tag (Gene) | ETAE_1158 |
| Coordinates (Strand) | 1257412..1258584 (+) |
| NCBI ID | 502611022 |
| RefSeq | NC_013508 |
| Uniprot ID | D0ZFG9_EDWTE, A0A0H3DP76_EDWTF |
| KEGG ID | etd:ETAF_1079, etr:ETAE_1156 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_0886 (esrB) promoter | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF418 | PF04235.14 | 3e-40 | 227..384 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-20 | MAEMPVAAQRGRAPRIAALD |
| Transmembrane helix | 21-40 | ALRGLALLGILLMNIVGFAL |
| Outside | 41-59 | PKAAYLNPAYQGIPSAWDQ |
| Transmembrane helix | 60-82 | GVWLLLSLTVQGKFLALFALLFG |
| Inside | 83-94 | AGLQLQLPRGGR |
| Transmembrane helix | 95-114 | WIQARLGWLMLFGLLHTLLL |
| Outside | 115-118 | WDGD |
| Transmembrane helix | 119-135 | ILLVYGLIGLMCWRTIR |
| Inside | 136-141 | DADSAR |
| Transmembrane helix | 142-164 | ALLYRGATLYAVGLGMLLLLAAM |
| Outside | 165-210 | SRAEPHGFWQVGYAEIQYETYWKLRGGVEAWRNRSELLISNLLALA |
| Transmembrane helix | 211-233 | VQYGWQLCGVMLLGAGLMRCGWL |
| Inside | 234-244 | RGRFSSAHYRR |
| Transmembrane helix | 245-267 | CGAGLLLSGLALQALGSGLQWYL |
| Outside | 268-276 | RWDFRWCGY |
| Transmembrane helix | 277-299 | LLQLPGELAAPLMMLGYVALIYA |
| Inside | 300-319 | AWPRLSRWRLTAALQRVGRM |
| Transmembrane helix | 320-339 | ALSNYLLQTLICTTLFYRWG |
| Outside | 340-348 | LFNHAGRAA |
| Transmembrane helix | 349-371 | LLLWVPPVWGGCLLFATLWLRVF |
| Inside | 372-390 | RQGPVEWLWRRLTGLSLRV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9621 | - | - |
Protein sequence of regulator: 391 a.a. .
>REG0231 NC_013508:1257412-1258584 [Edwardsiella tarda EIB202]
MAEMPVAAQRGRAPRIAALDALRGLALLGILLMNIVGFALPKAAYLNPAYQGIPSAWDQGVWLLLSLTVQGKFLALFALL
FGAGLQLQLPRGGRWIQARLGWLMLFGLLHTLLLWDGDILLVYGLIGLMCWRTIRDADSARALLYRGATLYAVGLGMLLL
LAAMSRAEPHGFWQVGYAEIQYETYWKLRGGVEAWRNRSELLISNLLALAVQYGWQLCGVMLLGAGLMRCGWLRGRFSSA
HYRRCGAGLLLSGLALQALGSGLQWYLRWDFRWCGYLLQLPGELAAPLMMLGYVALIYAAWPRLSRWRLTAALQRVGRMA
LSNYLLQTLICTTLFYRWGLFNHAGRAALLLWVPPVWGGCLLFATLWLRVFRQGPVEWLWRRLTGLSLRV*
MAEMPVAAQRGRAPRIAALDALRGLALLGILLMNIVGFALPKAAYLNPAYQGIPSAWDQGVWLLLSLTVQGKFLALFALL
FGAGLQLQLPRGGRWIQARLGWLMLFGLLHTLLLWDGDILLVYGLIGLMCWRTIRDADSARALLYRGATLYAVGLGMLLL
LAAMSRAEPHGFWQVGYAEIQYETYWKLRGGVEAWRNRSELLISNLLALAVQYGWQLCGVMLLGAGLMRCGWLRGRFSSA
HYRRCGAGLLLSGLALQALGSGLQWYLRWDFRWCGYLLQLPGELAAPLMMLGYVALIYAAWPRLSRWRLTAALQRVGRMA
LSNYLLQTLICTTLFYRWGLFNHAGRAALLLWVPPVWGGCLLFATLWLRVFRQGPVEWLWRRLTGLSLRV*
Nucleotide sequence of regulator: 1173 bp .
>REG0231 NC_013508:1257412-1258584 [Edwardsiella tarda EIB202]
ATGGCCGAGATGCCTGTGGCGGCGCAGCGGGGACGAGCGCCCCGGATCGCGGCGCTGGATGCCCTGCGCGGCCTCGCGCT
GCTGGGGATCCTGCTGATGAATATCGTCGGCTTTGCCCTGCCAAAGGCGGCGTACCTGAACCCCGCCTATCAGGGGATCC
CCTCCGCCTGGGATCAGGGGGTATGGCTGCTGCTCAGCCTGACGGTGCAGGGCAAGTTTCTGGCCCTGTTCGCGCTGCTG
TTCGGCGCTGGCCTGCAGCTACAGCTGCCGCGCGGCGGGCGCTGGATCCAGGCGCGTTTGGGATGGCTGATGCTGTTTGG
GCTGCTGCATACCCTGCTGCTGTGGGATGGCGACATTCTCCTGGTCTATGGCCTGATCGGCCTGATGTGCTGGCGGACGA
TCCGCGACGCCGATTCGGCCCGCGCTCTGCTGTACCGCGGGGCGACGCTGTATGCCGTCGGGCTGGGGATGCTGCTGCTG
CTGGCCGCGATGAGCCGCGCGGAGCCACACGGCTTTTGGCAGGTCGGCTATGCCGAGATCCAGTATGAGACCTACTGGAA
GCTGCGCGGGGGCGTCGAGGCCTGGCGTAACCGCAGCGAGCTGTTGATAAGCAATCTGCTGGCGCTGGCGGTGCAGTATG
GCTGGCAGCTGTGCGGCGTGATGCTGCTGGGCGCGGGACTGATGCGCTGCGGTTGGCTGCGCGGCCGCTTTTCCTCTGCC
CACTACCGGCGCTGCGGGGCCGGGCTGCTGCTGAGCGGGCTGGCGCTTCAGGCGCTGGGCAGTGGTCTGCAGTGGTATCT
GCGCTGGGACTTTCGCTGGTGCGGCTACCTGCTACAGCTGCCCGGTGAGCTGGCGGCGCCGCTGATGATGCTGGGCTATG
TGGCGCTGATCTACGCCGCTTGGCCGCGCCTGTCGCGCTGGCGGCTGACCGCCGCGCTGCAGCGGGTTGGGCGTATGGCC
CTGAGCAACTATCTGCTGCAGACGCTGATCTGCACTACGCTGTTTTATCGATGGGGGCTATTCAATCACGCCGGGCGCGC
GGCGCTGCTGCTGTGGGTGCCGCCGGTGTGGGGGGGCTGTCTGCTGTTTGCGACGCTCTGGCTGCGCGTCTTCCGTCAGG
GGCCCGTAGAGTGGCTGTGGCGCCGTCTGACCGGACTGTCTCTGCGCGTCTGA
ATGGCCGAGATGCCTGTGGCGGCGCAGCGGGGACGAGCGCCCCGGATCGCGGCGCTGGATGCCCTGCGCGGCCTCGCGCT
GCTGGGGATCCTGCTGATGAATATCGTCGGCTTTGCCCTGCCAAAGGCGGCGTACCTGAACCCCGCCTATCAGGGGATCC
CCTCCGCCTGGGATCAGGGGGTATGGCTGCTGCTCAGCCTGACGGTGCAGGGCAAGTTTCTGGCCCTGTTCGCGCTGCTG
TTCGGCGCTGGCCTGCAGCTACAGCTGCCGCGCGGCGGGCGCTGGATCCAGGCGCGTTTGGGATGGCTGATGCTGTTTGG
GCTGCTGCATACCCTGCTGCTGTGGGATGGCGACATTCTCCTGGTCTATGGCCTGATCGGCCTGATGTGCTGGCGGACGA
TCCGCGACGCCGATTCGGCCCGCGCTCTGCTGTACCGCGGGGCGACGCTGTATGCCGTCGGGCTGGGGATGCTGCTGCTG
CTGGCCGCGATGAGCCGCGCGGAGCCACACGGCTTTTGGCAGGTCGGCTATGCCGAGATCCAGTATGAGACCTACTGGAA
GCTGCGCGGGGGCGTCGAGGCCTGGCGTAACCGCAGCGAGCTGTTGATAAGCAATCTGCTGGCGCTGGCGGTGCAGTATG
GCTGGCAGCTGTGCGGCGTGATGCTGCTGGGCGCGGGACTGATGCGCTGCGGTTGGCTGCGCGGCCGCTTTTCCTCTGCC
CACTACCGGCGCTGCGGGGCCGGGCTGCTGCTGAGCGGGCTGGCGCTTCAGGCGCTGGGCAGTGGTCTGCAGTGGTATCT
GCGCTGGGACTTTCGCTGGTGCGGCTACCTGCTACAGCTGCCCGGTGAGCTGGCGGCGCCGCTGATGATGCTGGGCTATG
TGGCGCTGATCTACGCCGCTTGGCCGCGCCTGTCGCGCTGGCGGCTGACCGCCGCGCTGCAGCGGGTTGGGCGTATGGCC
CTGAGCAACTATCTGCTGCAGACGCTGATCTGCACTACGCTGTTTTATCGATGGGGGCTATTCAATCACGCCGGGCGCGC
GGCGCTGCTGCTGTGGGTGCCGCCGGTGTGGGGGGGCTGTCTGCTGTTTGCGACGCTCTGGCTGCGCGTCTTCCGTCAGG
GGCCCGTAGAGTGGCTGTGGCGCCGTCTGACCGGACTGTCTCTGCGCGTCTGA