Detailed information of regulator
Summary
| Regulator ID | REG0226  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (231 a.a.); Nucleotide sequence (693 bp) |
| Description | Putative repressors of esrB as identified by Tn-seq. |
| Reference |
External database links
| Locus tag (Gene) | ETAE_1614 |
| Coordinates (Strand) | 1721332..1722024 (-) |
| NCBI ID | 754045576 |
| RefSeq | NC_013508 |
| Uniprot ID | UPI00045D3F3C |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_0886 (esrB) promoter | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Peptidase_S24 | PF00717.25 | 7.5e-17 | 132..219 |
| HTH_3 | PF01381.24 | 2.4e-06 | 14..63 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-230 | MNENTFADRLSLAMAEAKMTQASLAEAVGMAQPSVWKLTSGKAKSSRKVVEIAAVLGVQPEWLSSGRGPMRKDNGVVISSLPLPKDDSYIISVMDISYSCGPGSNNKDYPDIIRSISLEPDYARRVFGGRSSNVIRAINANGDSMKGTIDPEDLVFIDISVKYFDGDGVYAFTYGSTSHIKRLQMVKNTLTVISDNPAYANWSIEKGDEDQMHINGKVIINWPMRLVRFA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8678 | - | - |
Protein sequence of regulator: 231 a.a. .
>REG0226 NC_013508:c1722024-1721332 [Edwardsiella tarda EIB202]
MNENTFADRLSLAMAEAKMTQASLAEAVGMAQPSVWKLTSGKAKSSRKVVEIAAVLGVQPEWLSSGRGPMRKDNGVVISS
LPLPKDDSYIISVMDISYSCGPGSNNKDYPDIIRSISLEPDYARRVFGGRSSNVIRAINANGDSMKGTIDPEDLVFIDIS
VKYFDGDGVYAFTYGSTSHIKRLQMVKNTLTVISDNPAYANWSIEKGDEDQMHINGKVIINWPMRLVRFA*
MNENTFADRLSLAMAEAKMTQASLAEAVGMAQPSVWKLTSGKAKSSRKVVEIAAVLGVQPEWLSSGRGPMRKDNGVVISS
LPLPKDDSYIISVMDISYSCGPGSNNKDYPDIIRSISLEPDYARRVFGGRSSNVIRAINANGDSMKGTIDPEDLVFIDIS
VKYFDGDGVYAFTYGSTSHIKRLQMVKNTLTVISDNPAYANWSIEKGDEDQMHINGKVIINWPMRLVRFA*
Nucleotide sequence of regulator: 693 bp .
>REG0226 NC_013508:c1722024-1721332 [Edwardsiella tarda EIB202]
ATGAACGAAAATACATTTGCAGACAGACTTAGCCTTGCCATGGCAGAGGCAAAAATGACACAGGCCTCTCTGGCGGAGGC
TGTAGGAATGGCTCAACCCAGCGTATGGAAACTGACATCAGGAAAGGCTAAGAGCTCTCGCAAAGTTGTCGAGATCGCGG
CGGTGCTTGGGGTTCAGCCTGAATGGTTATCATCTGGACGGGGGCCAATGAGGAAGGATAATGGGGTTGTGATCTCATCC
CTGCCATTACCGAAGGATGACTCTTATATCATAAGCGTCATGGATATTTCTTACAGCTGCGGTCCAGGGAGCAACAACAA
AGATTACCCGGATATCATCCGGTCTATATCACTAGAGCCTGATTACGCCCGTAGGGTGTTTGGTGGTCGCTCGAGCAACG
TCATTCGTGCGATAAATGCCAACGGCGATAGTATGAAAGGAACGATTGACCCCGAAGATTTAGTCTTTATCGATATATCG
GTAAAATACTTCGACGGGGATGGGGTTTACGCATTTACCTATGGAAGCACCTCACATATCAAGAGGCTACAAATGGTAAA
AAACACCCTCACTGTCATCTCTGATAACCCCGCGTATGCTAACTGGAGCATTGAAAAAGGAGATGAAGACCAGATGCACA
TCAACGGAAAAGTTATCATTAATTGGCCAATGCGATTAGTCAGATTCGCATAG
ATGAACGAAAATACATTTGCAGACAGACTTAGCCTTGCCATGGCAGAGGCAAAAATGACACAGGCCTCTCTGGCGGAGGC
TGTAGGAATGGCTCAACCCAGCGTATGGAAACTGACATCAGGAAAGGCTAAGAGCTCTCGCAAAGTTGTCGAGATCGCGG
CGGTGCTTGGGGTTCAGCCTGAATGGTTATCATCTGGACGGGGGCCAATGAGGAAGGATAATGGGGTTGTGATCTCATCC
CTGCCATTACCGAAGGATGACTCTTATATCATAAGCGTCATGGATATTTCTTACAGCTGCGGTCCAGGGAGCAACAACAA
AGATTACCCGGATATCATCCGGTCTATATCACTAGAGCCTGATTACGCCCGTAGGGTGTTTGGTGGTCGCTCGAGCAACG
TCATTCGTGCGATAAATGCCAACGGCGATAGTATGAAAGGAACGATTGACCCCGAAGATTTAGTCTTTATCGATATATCG
GTAAAATACTTCGACGGGGATGGGGTTTACGCATTTACCTATGGAAGCACCTCACATATCAAGAGGCTACAAATGGTAAA
AAACACCCTCACTGTCATCTCTGATAACCCCGCGTATGCTAACTGGAGCATTGAAAAAGGAGATGAAGACCAGATGCACA
TCAACGGAAAAGTTATCATTAATTGGCCAATGCGATTAGTCAGATTCGCATAG