Detailed information of regulator
Summary
| Regulator ID | REG0217  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Homologous recombination (map03440) |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (203 a.a.); Nucleotide sequence (609 bp) |
| Description | Putative repressors of esrB as identified by Tn-seq. |
| Reference |
External database links
| Locus tag (Gene) | ETAE_1439 (ruvA) |
| Coordinates (Strand) | 1550776..1551384 (+) |
| NCBI ID | 502611302 |
| RefSeq | NC_013508 |
| Uniprot ID | D0ZH04_EDWTE, A0A0H3DPZ9_EDWTF |
| KEGG ID | etd:ETAF_1334, etr:ETAE_1439 |
| KEGG pathway (determined by BlastKOALA) | map03440 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_0886 (esrB) promoter | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| RuvA_N | PF01330.23 | 1.7e-20 | 2..62 |
| HHH_5 | PF14520.8 | 3.8e-17 | 72..130 |
| RuvA_C | PF07499.15 | 4.3e-12 | 158..200 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-202 | VIGRLRGTILEKQPPQVVLETHGVGYEVQMPMTCFYELPQVGSEAVIFTHFVVREDAQLLYGFNNKQERALFRELIKVNGVGPKLALAILSGMSAQQFVAAVEREEVAALVKLPGVGKKTAERLVVEMKDRFKGLSGDLFTPTDDLPPSTQPQAQADAEGEAVAALIALGYKPQEASRLVNRVTVAGADSETLIRDALRAAL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9955 | - | - |
Protein sequence of regulator: 203 a.a. .
>REG0217 NC_013508:1550776-1551384 [Edwardsiella tarda EIB202]
VIGRLRGTILEKQPPQVVLETHGVGYEVQMPMTCFYELPQVGSEAVIFTHFVVREDAQLLYGFNNKQERALFRELIKVNG
VGPKLALAILSGMSAQQFVAAVEREEVAALVKLPGVGKKTAERLVVEMKDRFKGLSGDLFTPTDDLPPSTQPQAQADAEG
EAVAALIALGYKPQEASRLVNRVTVAGADSETLIRDALRAAL*
VIGRLRGTILEKQPPQVVLETHGVGYEVQMPMTCFYELPQVGSEAVIFTHFVVREDAQLLYGFNNKQERALFRELIKVNG
VGPKLALAILSGMSAQQFVAAVEREEVAALVKLPGVGKKTAERLVVEMKDRFKGLSGDLFTPTDDLPPSTQPQAQADAEG
EAVAALIALGYKPQEASRLVNRVTVAGADSETLIRDALRAAL*
Nucleotide sequence of regulator: 609 bp .
>REG0217 NC_013508:1550776-1551384 [Edwardsiella tarda EIB202]
GTGATTGGTCGCCTCAGAGGAACTATTCTGGAAAAGCAGCCGCCGCAGGTGGTGCTGGAAACCCACGGCGTCGGCTATGA
GGTGCAGATGCCGATGACCTGCTTTTATGAGCTGCCGCAGGTTGGCAGTGAAGCGGTGATCTTTACCCACTTCGTGGTAC
GCGAAGACGCCCAGCTGCTGTACGGGTTCAACAATAAGCAGGAGCGCGCGCTGTTCCGCGAACTGATCAAGGTCAACGGC
GTCGGGCCCAAGCTGGCGCTGGCGATCCTGTCCGGGATGTCGGCGCAGCAGTTTGTCGCGGCGGTTGAGCGTGAAGAGGT
CGCGGCGCTGGTCAAGCTGCCGGGCGTGGGCAAGAAAACCGCCGAGCGGCTGGTGGTCGAGATGAAGGACCGCTTCAAGG
GGCTGAGCGGCGATCTGTTTACCCCGACGGATGACCTGCCGCCGAGCACCCAACCGCAGGCCCAGGCGGATGCCGAGGGC
GAGGCGGTTGCCGCGCTGATCGCGCTGGGCTACAAGCCACAGGAGGCCAGCCGCCTGGTGAACCGGGTGACGGTGGCCGG
CGCTGACAGTGAAACCCTGATCCGCGACGCGCTGCGCGCGGCGCTGTAA
GTGATTGGTCGCCTCAGAGGAACTATTCTGGAAAAGCAGCCGCCGCAGGTGGTGCTGGAAACCCACGGCGTCGGCTATGA
GGTGCAGATGCCGATGACCTGCTTTTATGAGCTGCCGCAGGTTGGCAGTGAAGCGGTGATCTTTACCCACTTCGTGGTAC
GCGAAGACGCCCAGCTGCTGTACGGGTTCAACAATAAGCAGGAGCGCGCGCTGTTCCGCGAACTGATCAAGGTCAACGGC
GTCGGGCCCAAGCTGGCGCTGGCGATCCTGTCCGGGATGTCGGCGCAGCAGTTTGTCGCGGCGGTTGAGCGTGAAGAGGT
CGCGGCGCTGGTCAAGCTGCCGGGCGTGGGCAAGAAAACCGCCGAGCGGCTGGTGGTCGAGATGAAGGACCGCTTCAAGG
GGCTGAGCGGCGATCTGTTTACCCCGACGGATGACCTGCCGCCGAGCACCCAACCGCAGGCCCAGGCGGATGCCGAGGGC
GAGGCGGTTGCCGCGCTGATCGCGCTGGGCTACAAGCCACAGGAGGCCAGCCGCCTGGTGAACCGGGTGACGGTGGCCGG
CGCTGACAGTGAAACCCTGATCCGCGACGCGCTGCGCGCGGCGCTGTAA