Detailed information of regulator
Summary
| Regulator ID | REG0225  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (291 a.a.); Nucleotide sequence (873 bp) |
| Description | Putative repressors of esrB as identified by Tn-seq. |
| Reference |
External database links
| Locus tag (Gene) | ETAE_2468 |
| Coordinates (Strand) | 2598484..2599356 (-) |
| NCBI ID | 504339400 |
| RefSeq | NC_013508 |
| Uniprot ID | A0A0H3DXD9_EDWTF |
| KEGG ID | etd:ETAF_ple019 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_0886 (esrB) promoter | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MNKRKY |
| Transmembrane helix | 7-24 | VFLLFFIFPICAFGADGA |
| Outside | 25-290 | EDSVPVFTTPWSRLFSGSEARLSTAITYNAPLSNQDKYIPVSYTESETKNIYNQRVFVSFQYSPLSSFFANLTVRTPLQNINRYRADFVYSFGYDDWRPGTFSLVYSNYGDNNKFFPQEGDRRTKIEQGMITAAYKFSLPNSWNKNILIYPSDSLTCQIGYLYGPRYYSTQESRIRKGKSVLLGSCGYTLKHNYFFRVSTFFYPDRSQQQPWDADYTYSIGYVSGYQPGDLSISYSNYSGTRYFWRGDRNANFRDGTISITWALPF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.8891 | pos: 20-21. AFG-AD. | MNKRKYVFLLFFIFPICAFG |
Protein sequence of regulator: 291 a.a. .
>REG0225 NC_013508:c2599356-2598484 [Edwardsiella tarda EIB202]
MNKRKYVFLLFFIFPICAFGADGAEDSVPVFTTPWSRLFSGSEARLSTAITYNAPLSNQDKYIPVSYTESETKNIYNQRV
FVSFQYSPLSSFFANLTVRTPLQNINRYRADFVYSFGYDDWRPGTFSLVYSNYGDNNKFFPQEGDRRTKIEQGMITAAYK
FSLPNSWNKNILIYPSDSLTCQIGYLYGPRYYSTQESRIRKGKSVLLGSCGYTLKHNYFFRVSTFFYPDRSQQQPWDADY
TYSIGYVSGYQPGDLSISYSNYSGTRYFWRGDRNANFRDGTISITWALPF*
MNKRKYVFLLFFIFPICAFGADGAEDSVPVFTTPWSRLFSGSEARLSTAITYNAPLSNQDKYIPVSYTESETKNIYNQRV
FVSFQYSPLSSFFANLTVRTPLQNINRYRADFVYSFGYDDWRPGTFSLVYSNYGDNNKFFPQEGDRRTKIEQGMITAAYK
FSLPNSWNKNILIYPSDSLTCQIGYLYGPRYYSTQESRIRKGKSVLLGSCGYTLKHNYFFRVSTFFYPDRSQQQPWDADY
TYSIGYVSGYQPGDLSISYSNYSGTRYFWRGDRNANFRDGTISITWALPF*
Nucleotide sequence of regulator: 873 bp .
>REG0225 NC_013508:c2599356-2598484 [Edwardsiella tarda EIB202]
ATGAATAAGAGAAAATATGTTTTTTTATTGTTTTTTATTTTTCCGATATGTGCTTTTGGTGCTGATGGTGCAGAGGACTC
TGTTCCCGTGTTTACCACCCCGTGGTCTCGCCTTTTTTCTGGTTCAGAAGCGAGATTGTCTACAGCTATTACTTATAATG
CACCATTGTCTAATCAAGACAAGTATATACCAGTAAGTTATACTGAGAGTGAGACCAAAAACATATATAACCAAAGAGTC
TTTGTTAGTTTTCAGTACTCCCCATTAAGCTCTTTTTTTGCTAATTTGACGGTTAGGACACCATTGCAAAATATTAATCG
TTATAGAGCTGATTTTGTTTATAGTTTTGGCTATGATGATTGGAGGCCAGGAACATTCAGCCTAGTATATAGCAATTATG
GTGACAATAATAAATTCTTCCCACAAGAAGGTGATCGCAGAACAAAAATAGAGCAAGGAATGATTACTGCGGCATATAAG
TTTTCGCTTCCGAACTCATGGAATAAAAATATATTGATATATCCTAGTGATAGCCTAACATGCCAAATAGGCTATTTATA
TGGCCCAAGATATTACTCGACACAAGAGAGTAGAATACGGAAAGGGAAGAGTGTATTGCTTGGAAGTTGCGGGTATACAT
TAAAACATAATTATTTTTTCAGGGTTTCGACGTTCTTTTACCCAGATAGATCTCAGCAACAACCATGGGATGCAGACTAT
ACCTATAGCATTGGCTATGTTTCAGGTTATCAACCTGGTGATTTATCAATTTCATATAGTAACTATAGCGGAACACGGTA
TTTTTGGCGAGGTGATCGGAATGCTAACTTTCGAGATGGGACCATTAGTATTACTTGGGCTCTCCCATTCTGA
ATGAATAAGAGAAAATATGTTTTTTTATTGTTTTTTATTTTTCCGATATGTGCTTTTGGTGCTGATGGTGCAGAGGACTC
TGTTCCCGTGTTTACCACCCCGTGGTCTCGCCTTTTTTCTGGTTCAGAAGCGAGATTGTCTACAGCTATTACTTATAATG
CACCATTGTCTAATCAAGACAAGTATATACCAGTAAGTTATACTGAGAGTGAGACCAAAAACATATATAACCAAAGAGTC
TTTGTTAGTTTTCAGTACTCCCCATTAAGCTCTTTTTTTGCTAATTTGACGGTTAGGACACCATTGCAAAATATTAATCG
TTATAGAGCTGATTTTGTTTATAGTTTTGGCTATGATGATTGGAGGCCAGGAACATTCAGCCTAGTATATAGCAATTATG
GTGACAATAATAAATTCTTCCCACAAGAAGGTGATCGCAGAACAAAAATAGAGCAAGGAATGATTACTGCGGCATATAAG
TTTTCGCTTCCGAACTCATGGAATAAAAATATATTGATATATCCTAGTGATAGCCTAACATGCCAAATAGGCTATTTATA
TGGCCCAAGATATTACTCGACACAAGAGAGTAGAATACGGAAAGGGAAGAGTGTATTGCTTGGAAGTTGCGGGTATACAT
TAAAACATAATTATTTTTTCAGGGTTTCGACGTTCTTTTACCCAGATAGATCTCAGCAACAACCATGGGATGCAGACTAT
ACCTATAGCATTGGCTATGTTTCAGGTTATCAACCTGGTGATTTATCAATTTCATATAGTAACTATAGCGGAACACGGTA
TTTTTGGCGAGGTGATCGGAATGCTAACTTTCGAGATGGGACCATTAGTATTACTTGGGCTCTCCCATTCTGA