SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0210
Regulator type	protein
Regulatory pathway (from literatures)	TCS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Metabolic pathways (map01100); Biosynthesis of cofactors (map01240); Pyrimidine metabolism (map00240)
Related T6SS	T6SS00372 (Type i4b)
Strain	Edwardsiella tarda EIB202
Replicon	chromosome
Sequence	Protein sequence (241 a.a.); Nucleotide sequence (723 bp)
Description	Putative repressors of esrB as identified by Tn-seq.
Reference	[1] Yin K, Guan Y, Ma R, et al. Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida. PLoS Pathog. 2018 Aug 31;14(8):e1007272. doi: 10.1371/journal.ppat.1007272. eCollection 2018 Aug. PMID: 30169545

External database links

Locus tag (Gene)	ETAE_2082 (pyrF)
Coordinates (Strand)	2196136..2196858 (-)
NCBI ID	502611934
RefSeq	NC_013508
Uniprot ID	D0Z9E1_EDWTE, A0A0H3DTM3_EDWTF
KEGG ID	etd:ETAF_1881, etr:ETAE_2082
KEGG pathway (determined by BlastKOALA)	map01100; map01240; map00240
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
ETAE_0886 (esrB) promoter	↑	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
OMPdecase	PF00215.26	6.1e-51	13..235

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-240	MSISTPMRAHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQQRGFDVFLDLKFHDIPNTVARAVAAAADLGVWMVNVHASGSLRMMSAAREALRPYGKEAPLLIAVTVLTSMEQADLQAIGIDEAPADYAERLARLTQQCGLDGVVCSAQEAVRIKAACGSAFKLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9893	-	-

Protein sequence of regulator: 241 a.a. .

                                    >REG0210 NC_013508:c2196858-2196136 [Edwardsiella tarda EIB202]
MSISTPMRAHSHSPIVVALDYADRNAALALVDALDPRDCRLKVGKEMFTLFGPEWVRTLQQRGFDVFLDLKFHDIPNTVA
RAVAAAADLGVWMVNVHASGSLRMMSAAREALRPYGKEAPLLIAVTVLTSMEQADLQAIGIDEAPADYAERLARLTQQCG
LDGVVCSAQEAVRIKAACGSAFKLVTPGIRPAGSEAGDQRRIMTPQQAVAAGVDYMVIGRPITQAAQPAEALRAILATLA
*

                                 

Nucleotide sequence of regulator: 723 bp .

                                    >REG0210 NC_013508:c2196858-2196136 [Edwardsiella tarda EIB202]
ATGTCAATATCTACTCCGATGCGTGCCCATAGCCACTCTCCGATCGTTGTCGCCCTTGATTATGCCGATCGCAACGCCGC
CCTTGCGCTGGTGGATGCGCTCGATCCGCGCGACTGTCGGCTGAAGGTCGGTAAGGAAATGTTCACCCTGTTCGGCCCCG
AGTGGGTTCGCACTCTGCAGCAGCGCGGATTCGATGTTTTTCTGGATCTTAAGTTTCACGATATCCCCAATACCGTCGCA
CGCGCCGTGGCGGCGGCGGCGGATCTGGGGGTGTGGATGGTCAACGTGCACGCCAGCGGCAGCCTACGCATGATGAGCGC
GGCCCGAGAGGCGCTGCGGCCCTACGGCAAGGAGGCGCCGCTGCTGATCGCCGTGACGGTGCTGACCAGCATGGAGCAGG
CCGATCTGCAGGCGATCGGTATCGACGAGGCGCCGGCCGACTATGCCGAGCGCCTGGCGCGTCTGACGCAGCAGTGCGGG
CTGGATGGGGTGGTCTGCTCGGCCCAGGAGGCGGTGCGCATCAAAGCCGCCTGTGGCTCCGCATTCAAACTGGTGACGCC
GGGGATCCGGCCCGCGGGCAGTGAGGCCGGCGATCAGCGTCGGATTATGACGCCGCAGCAGGCCGTCGCCGCCGGGGTCG
ACTATATGGTGATCGGACGTCCGATCACCCAAGCGGCGCAGCCGGCCGAGGCGCTGCGCGCCATTCTGGCGACGCTGGCC
TAA

                                