SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0242
Regulator type	protein
Regulatory pathway (from literatures)	TCS regulatory pathway
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00372 (Type i4b)
Strain	Edwardsiella tarda EIB202
Replicon	chromosome
Sequence	Protein sequence (513 a.a.); Nucleotide sequence (1539 bp)
Description	MviN mediates the regulation of osmotic pressure on esrB transcription. The deletion of mviN slightly increased the productions of T3/T6SS (EseB/C/D), while the over-expression of mviN significantly decreased those proteins' yield
Reference	[1] Yin K, Guan Y, Ma R, et al. Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida. PLoS Pathog. 2018 Aug 31;14(8):e1007272. doi: 10.1371/journal.ppat.1007272. eCollection 2018 Aug. PMID: 30169545 [2] Yin K, Zhang J, Ma J, et al. MviN mediates the regulation of environmental osmotic pressure on esrB to control the virulence in the marine fish pathogen Edwardsiella piscicida. Microbiol Res. 2020 Oct;239:126528. doi: 10.1016/j.micres.2020.126528. Epub 2020 Jun 27. PMID: 32622286

External database links

Locus tag (Gene)	ETAE_1425 (mviN)
Coordinates (Strand)	1535368..1536906 (+)
NCBI ID	502611288
RefSeq	NC_013508
Uniprot ID	D0ZGZ0_EDWTE, A0A0H3DS91_EDWTF
KEGG ID	etd:ETAF_1320, etr:ETAE_1425
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
ETAE_0886 (esrB) promoter	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
MurJ	PF03023.16	3.7e-152	28..477

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-25	MNLLKSLAAVSSMTLFSRVLGFARD
Transmembrane helix	26-45	ALVARIFGAGMATDAFFVAF
Inside	46-87	KLPNLLRRIFAEGAFSQAFVPILAEYKNQQGEQATQTFIAYV
Transmembrane helix	88-110	SGLLTLILALVTLLGMLAAPWVI
Outside	111-129	YATAPGFADTPDKFALTSA
Transmembrane helix	130-152	LLRITFPYILLISLASMAGAVLN
Inside	153-158	TWNRFS
Transmembrane helix	159-181	VPAFAPTLLNVSMIGFALFVAPY
Outside	182-185	CHPP
Transmembrane helix	186-203	ILALAWAVLVGGVLQLGY
Inside	204-235	QLPHLKKIGMLVLPRLNLHDRGVWRVLKLMGP
Transmembrane helix	236-258	AIVGVSVSQISLIINTIFASFLV
Outside	259-272	SGSVSWMYYADRLM
Transmembrane helix	273-295	ELPSGVLGVALGTILLPSLAKSF
Inside	296-314	SSGRLDDYNRLLDWGLRLC
Transmembrane helix	315-337	FLLALPCAVALGVIAKPLTVALF
Outside	338-351	QYGKFSAFDAAMTQ
Transmembrane helix	352-374	RALVAYSIGLLGIIMVKILAPGF
Inside	375-385	YSRQDIKTPVK
Transmembrane helix	386-403	IAIVTLILTQVMNLIFIG
Outside	404-406	PLK
Transmembrane helix	407-429	HAGLSLSIGLAACLNAGLLYWQL
Inside	430-441	RRQRLFTPQKGW
Transmembrane helix	442-464	LGFLGKLVLAVLVMTAVLIGMMW
Outside	465-478	LMPAWDIGNMPYRL
Transmembrane helix	479-501	LRLVGLVVAGAVSYFAVLALLGF
Inside	502-512	RLRDFTRSAVL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8331	-	-

Protein sequence of regulator: 513 a.a. .

                                    >REG0242 NC_013508:1535368-1536906 [Edwardsiella tarda EIB202]
MNLLKSLAAVSSMTLFSRVLGFARDALVARIFGAGMATDAFFVAFKLPNLLRRIFAEGAFSQAFVPILAEYKNQQGEQAT
QTFIAYVSGLLTLILALVTLLGMLAAPWVIYATAPGFADTPDKFALTSALLRITFPYILLISLASMAGAVLNTWNRFSVP
AFAPTLLNVSMIGFALFVAPYCHPPILALAWAVLVGGVLQLGYQLPHLKKIGMLVLPRLNLHDRGVWRVLKLMGPAIVGV
SVSQISLIINTIFASFLVSGSVSWMYYADRLMELPSGVLGVALGTILLPSLAKSFSSGRLDDYNRLLDWGLRLCFLLALP
CAVALGVIAKPLTVALFQYGKFSAFDAAMTQRALVAYSIGLLGIIMVKILAPGFYSRQDIKTPVKIAIVTLILTQVMNLI
FIGPLKHAGLSLSIGLAACLNAGLLYWQLRRQRLFTPQKGWLGFLGKLVLAVLVMTAVLIGMMWLMPAWDIGNMPYRLLR
LVGLVVAGAVSYFAVLALLGFRLRDFTRSAVL*

                                 

Nucleotide sequence of regulator: 1539 bp .

                                    >REG0242 NC_013508:1535368-1536906 [Edwardsiella tarda EIB202]
ATGAATCTACTTAAATCACTGGCTGCGGTCAGCTCAATGACCCTCTTTTCGCGCGTGCTGGGGTTTGCCCGCGACGCGCT
GGTCGCCAGGATCTTTGGCGCTGGGATGGCGACCGATGCCTTTTTTGTGGCCTTCAAGCTGCCCAACCTGCTACGGCGGA
TCTTTGCCGAGGGCGCCTTTTCCCAGGCCTTTGTGCCGATCCTGGCGGAGTATAAAAACCAGCAGGGCGAGCAGGCGACG
CAGACCTTTATCGCCTACGTCAGCGGTCTATTGACCCTGATTTTGGCGCTGGTGACGCTGCTCGGTATGCTGGCGGCCCC
CTGGGTTATCTATGCCACCGCGCCCGGCTTCGCCGATACGCCGGACAAGTTTGCGCTGACCAGCGCGCTGCTGCGCATCA
CCTTTCCCTATATCTTGCTCATCTCCTTGGCCTCGATGGCCGGCGCGGTGCTGAATACCTGGAACCGTTTTTCGGTACCG
GCCTTTGCGCCGACGCTGCTGAACGTCAGCATGATCGGCTTTGCGCTGTTTGTGGCGCCGTACTGCCATCCGCCGATCCT
GGCGCTGGCCTGGGCCGTGCTGGTGGGTGGCGTGCTGCAGCTGGGGTATCAGCTGCCGCACCTGAAGAAGATAGGCATGC
TGGTGCTGCCGCGCCTCAACCTGCACGATCGCGGGGTATGGCGGGTGCTCAAGCTGATGGGGCCGGCGATTGTCGGGGTT
TCTGTCAGCCAAATCTCGCTGATCATCAATACTATTTTCGCCTCTTTCCTGGTCTCGGGCTCCGTTTCCTGGATGTACTA
TGCCGACCGCCTGATGGAGCTGCCGTCGGGGGTGCTGGGGGTCGCGCTGGGGACGATTCTGCTGCCGTCGCTGGCCAAAA
GCTTCTCCTCTGGCCGCTTGGACGACTATAACCGTCTGCTGGACTGGGGGCTGCGCCTCTGCTTTCTGCTGGCGCTGCCC
TGCGCCGTGGCGCTGGGAGTGATCGCGAAGCCGCTGACGGTGGCGCTGTTCCAGTATGGTAAATTCAGCGCCTTTGATGC
GGCGATGACCCAGCGAGCGCTGGTGGCCTACTCCATCGGCCTGCTCGGGATTATCATGGTGAAGATCCTGGCGCCGGGCT
TTTACTCTCGCCAGGATATCAAGACGCCGGTGAAGATCGCCATCGTCACGCTGATCCTGACCCAGGTGATGAACCTGATC
TTTATCGGTCCCCTAAAGCATGCCGGGCTGTCGCTCTCCATCGGCCTGGCCGCCTGCCTGAATGCCGGTCTGCTGTACTG
GCAGCTGCGGCGTCAGCGTCTGTTCACGCCGCAGAAGGGGTGGTTGGGATTCCTGGGTAAGCTGGTGCTGGCGGTGCTGG
TGATGACGGCGGTGCTGATCGGCATGATGTGGCTGATGCCGGCGTGGGATATCGGAAATATGCCTTATCGCCTGCTGCGT
CTGGTGGGGCTGGTGGTGGCCGGCGCGGTCAGCTACTTTGCGGTGCTGGCGCTGCTGGGATTCCGCCTGCGCGACTTTAC
CCGCAGCGCGGTGCTATAA

                                