Detailed information of regulator
Summary
| Regulator ID | REG0247  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Transcription regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (136 a.a.); Nucleotide sequence (408 bp) |
| Description | H-NS1505 could down-regulate the expression of evpB and evpC |
| Reference |
External database links
| Locus tag (Gene) | ETAE_1505 (H-NS) |
| Coordinates (Strand) | 1615099..1615506 (-) |
| NCBI ID | 505274277 |
| RefSeq | NC_013508 |
| Uniprot ID | A0A0H3DSG2_EDWTF |
| KEGG ID | etd:ETAF_1395 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_2430 (evpB) | ↑ | ttatggccacctcacgctttgcccattatttaaaatcgatggtccgcgacaaaatcggcagctttatgagccgcagtgaatgtgaatattttctcaatcagtggatcagtaattatgtggtcggctcggatgatgctggccaggatatcaaggcgaaatatccattac The sites identified by H-NS-mediated DNase I protection are indictated in red |
| evpC promotor | ↑ | gggaagcccgcattgatgtcagtgatattccgggcaaaccgggtttctataaggcggtagcctatttaaaaccccatttccaactggaggggctgaccgcgtctttacgtctggtcgccgatctgccgccgccggcgcaaggctaacgatttagccgagtttgtttaccacgtatatccaaaacg The sites identified by H-NS-mediated DNase I protection are indictated in red |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Histone_HNS | PF00816.23 | 3.4e-18 | 34..127 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-135 | MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVKERREEDQQAQAEIEERTRKLQQYREMLIADGIDPNELLQTMNATKVAGKTKRAARPAKYQYVDENGETKTWTGQGRTPAVIKNAIEEQGKSLDDFLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9965 | - | - |
Protein sequence of regulator: 136 a.a. .
>REG0247 NC_013508:c1615506-1615099 [Edwardsiella tarda EIB202]
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVKERREEDQQAQAEIEERTRKLQQYREMLIADGIDPNELLQTMN
ATKVAGKTKRAARPAKYQYVDENGETKTWTGQGRTPAVIKNAIEEQGKSLDDFLI*
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVKERREEDQQAQAEIEERTRKLQQYREMLIADGIDPNELLQTMN
ATKVAGKTKRAARPAKYQYVDENGETKTWTGQGRTPAVIKNAIEEQGKSLDDFLI*
Nucleotide sequence of regulator: 408 bp .
>REG0247 NC_013508:c1615506-1615099 [Edwardsiella tarda EIB202]
ATGAGCGAAGCATTAAAAATTCTTAACAACATTCGTACTCTCCGCGCCCAGGCCCGTGAATGCACTCTGGAAACTCTAGA
AGAAATGCTGGAGAAACTGGAAGTCGTGGTAAAAGAGCGCCGCGAAGAAGATCAGCAAGCTCAGGCCGAGATCGAAGAGC
GTACCCGTAAACTGCAGCAGTACCGTGAAATGCTGATCGCAGACGGCATCGACCCGAATGAACTGCTGCAGACCATGAAT
GCCACCAAGGTTGCCGGCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATCAGTATGTTGACGAAAATGGCGAAACCAA
AACTTGGACCGGTCAGGGTCGTACCCCTGCCGTGATCAAGAACGCCATTGAAGAGCAGGGCAAGTCTCTGGACGACTTCC
TGATCTAA
ATGAGCGAAGCATTAAAAATTCTTAACAACATTCGTACTCTCCGCGCCCAGGCCCGTGAATGCACTCTGGAAACTCTAGA
AGAAATGCTGGAGAAACTGGAAGTCGTGGTAAAAGAGCGCCGCGAAGAAGATCAGCAAGCTCAGGCCGAGATCGAAGAGC
GTACCCGTAAACTGCAGCAGTACCGTGAAATGCTGATCGCAGACGGCATCGACCCGAATGAACTGCTGCAGACCATGAAT
GCCACCAAGGTTGCCGGCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATCAGTATGTTGACGAAAATGGCGAAACCAA
AACTTGGACCGGTCAGGGTCGTACCCCTGCCGTGATCAAGAACGCCATTGAAGAGCAGGGCAAGTCTCTGGACGACTTCC
TGATCTAA