Detailed information of regulator


Summary

Regulator ID REG0245 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Glutathione metabolism (map00480)
Related T6SS T6SS00372 experimental (Type i4b)
Strain Edwardsiella tarda EIB202
Replicon chromosome
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Description PepA directly binds to the esrB promotor and inhibits the transcription of esrB at an early state of growth, the transcription of EsrB-repressed genes ethA/ethB were repressed in ΔpepA, while the EsrB-activated T3/T6SS genes, i.e. esrB, evpA and eseB, were up-regulated in ΔpepA and over-expression of the pepA gene led to significantly decreased T3/T6SS production.
Reference
External database links

Locus tag (Gene) ETAE_3098 (PepA)
Coordinates (Strand) 3260322..3261833 (+)
NCBI ID 502612947
RefSeq NC_013508
Uniprot ID D0ZF58_EDWTE, A0A076LI78_9GAMM, A0A0H3DTQ8_EDWTF
KEGG ID etd:ETAF_2794, ete:ETEE_1287, etr:ETAE_3098
KEGG pathway (determined by BlastKOALA) map01100; map00480
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
ETAE_0886 (esrB) promoter ↑ tttaaagggtactccgaatcagtggcacactgaaaacttcatatagctcgctcggggcatttgaataaccagaccttcgctgaacgcctttttttggtatctaggatttttctaccacttttggcgtattgaattcgtcggtattcaaatttcggagtgagatttttaatctattcattccacggtttttcagcattattttggataaactgcgttacggtcaagcaaaactgctgtgtttgctgggatattcgcaggtttccagggagatgcgccaatgtttttcgcgccgtaaatgccctttggagtatggatcatcatgtcgaaaatgagctaattgatagcattttgctgagggtaagtctgctatttttaatcgtgaatggttgttttattcgcgagggtaagtaaaaaagaaagtgaattacaataataaattcatgagtctaggatttttctattcacgttattatttcagaatggaatatttttatatgcc
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Peptidase_M17 PF00883.23 2.6e-121 186..489
Peptidase_M17_N PF02789.19 1e-33 19..147
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-503 MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKVGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVVQKTINTLNDTGSMEAVCFLTELHVKGRNTYWKVRQAVETAKETLYTFDQLKSNKVEPRRPLRKMVFNVPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAGYLASQARQLADSYADAVTTRVIGEQQMKELGMNAYLAVGQGSQNESLMSVIEYKGSPNPDARPIVLVGKGLTFDAGGISLKPGEGMDEMKYDMCGAASVYGVMRMAAELRLPLNIVGVLAGCENMPGGRAYRPGDILTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPELVVDVATLTGACVIALGHHLTGLMANHNPLASELLSAAEQAGDRAWRLPLGEEFQEQLDSNFADMANIGGRPGGAITAACFLARFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLSQFLLNRAGLNGEE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9929 - -


  Protein sequence of regulator: 504 a.a.    .

>REG0245 NC_013508:3260322-3261833 [Edwardsiella tarda EIB202]
MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKVGQTLLLHHVPNVLSERILLIGCGK
ERELDERQYKQVVQKTINTLNDTGSMEAVCFLTELHVKGRNTYWKVRQAVETAKETLYTFDQLKSNKVEPRRPLRKMVFN
VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAGYLASQARQLADSYADAVTTRVIGEQQMKELGMNAYLAVG
QGSQNESLMSVIEYKGSPNPDARPIVLVGKGLTFDAGGISLKPGEGMDEMKYDMCGAASVYGVMRMAAELRLPLNIVGVL
AGCENMPGGRAYRPGDILTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPELVVDVATLTGACVIALGHHLTGLMANHN
PLASELLSAAEQAGDRAWRLPLGEEFQEQLDSNFADMANIGGRPGGAITAACFLARFTRKYNWAHLDIAGTAWRSGKAKG
ATGRPVALLSQFLLNRAGLNGEE*

  Nucleotide sequence of regulator: 1512 bp    .

>REG0245 NC_013508:3260322-3261833 [Edwardsiella tarda EIB202]
ATGGAGTTCAGTGTAAAGAGCGGTAGCCCGGAAAAACAGCGTAGCGCCTGTATCGTGGTCGGCGTGTTCGAACCGCGTCG
CCTGTCACCGATTGCCGAACAGCTCGACAAGATCAGCGACGGTTATATCAGCGCCCTGCTGCGCCGCGGCGAACTGGAAG
GCAAGGTGGGTCAAACCCTGTTGCTGCACCATGTCCCCAACGTACTCTCAGAGCGCATCCTGCTGATCGGCTGCGGCAAA
GAGCGTGAGCTGGACGAGCGCCAGTATAAGCAGGTGGTCCAGAAGACCATCAACACCCTCAACGATACCGGTTCGATGGA
GGCGGTGTGCTTCCTGACCGAGCTGCACGTCAAGGGCCGCAACACCTACTGGAAGGTGCGCCAGGCCGTCGAAACCGCCA
AAGAGACCCTCTACACCTTTGATCAGCTGAAGAGCAACAAGGTGGAGCCGCGCCGTCCGCTGCGCAAGATGGTGTTCAAC
GTGCCGACCCGCCGCGAGCTGACCAGCGGCGAACGCGCCATTCAGCACGGTCTGGCCATCGCCGCTGGGATCAAGGCCGC
CAAAGATCTCGGCAACATGCCGCCCAACATCTGTAACGCGGGCTACCTGGCCTCGCAGGCGCGTCAGCTGGCCGACAGCT
ACGCCGACGCCGTCACCACCCGGGTGATCGGCGAGCAGCAGATGAAAGAGCTGGGTATGAACGCCTATCTGGCCGTCGGG
CAGGGCTCGCAGAACGAGTCGCTGATGTCGGTGATCGAATACAAGGGCAGCCCGAACCCGGACGCCCGCCCCATCGTGCT
GGTCGGCAAAGGGCTGACCTTTGACGCCGGCGGCATCTCCCTGAAGCCGGGGGAAGGCATGGACGAGATGAAGTACGACA
TGTGCGGCGCCGCCTCTGTCTATGGCGTGATGCGCATGGCGGCCGAGCTGCGGCTGCCCCTCAACATCGTCGGGGTACTG
GCGGGCTGTGAAAACATGCCGGGCGGGCGCGCCTATCGGCCGGGCGACATTCTCACCACCATGTCCGGCCAGACCGTCGA
GGTGCTCAACACCGACGCCGAAGGGCGCCTGGTGCTGTGCGACGTGCTGACCTACGTCGAACGCTTCGAGCCGGAGCTGG
TGGTGGACGTCGCCACCCTGACCGGCGCCTGCGTCATCGCCCTGGGTCACCACCTGACCGGCCTGATGGCCAACCATAAC
CCGCTGGCTTCCGAGCTGCTGAGCGCCGCCGAGCAGGCGGGCGATCGCGCCTGGCGCCTGCCGCTGGGCGAGGAGTTCCA
AGAGCAGCTGGACTCCAACTTTGCCGACATGGCCAACATCGGCGGGCGCCCGGGCGGGGCGATCACCGCCGCCTGCTTCC
TGGCGCGCTTTACCCGCAAGTACAACTGGGCGCACCTGGATATCGCCGGTACCGCCTGGCGCTCCGGCAAGGCCAAGGGC
GCCACGGGCCGCCCGGTGGCGCTGCTGTCGCAGTTCCTGCTCAACCGCGCAGGGCTGAACGGCGAGGAGTGA