Detailed information of regulator
Summary
| Regulator ID | REG0218  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Biosynthesis of cofactors (map01240); Purine metabolism (map00230); Alanine, aspartate and glutamate metabolism (map00250) |
| Related T6SS | T6SS00372 (Type i4b) |
| Strain | Edwardsiella tarda EIB202 |
| Replicon | chromosome |
| Sequence | Protein sequence (433 a.a.); Nucleotide sequence (1299 bp) |
| Description | Putative repressors of esrB as identified by Tn-seq. |
| Reference |
External database links
| Locus tag (Gene) | ETAE_0358 (purA) |
| Coordinates (Strand) | 403538..404836 (+) |
| NCBI ID | 502610221 |
| RefSeq | NC_013508 |
| Uniprot ID | D0ZAP6_EDWTE, A0A0H3DPK7_EDWTF |
| KEGG ID | etr:ETAE_0358, etd:ETAF_0312 |
| KEGG pathway (determined by BlastKOALA) | map01100; map01240; map00230; map00250 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ETAE_0886 (esrB) promoter | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Adenylsucc_synt | PF00709.23 | 9.7e-180 | 6..424 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-432 | MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVVSIIANGVVLAPDALLREMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKESFAVKLKEIMEYHNFQLVNYYHVDAVDYQRVLDEVMAVADLLTSMVVDVADLLNKAYRNGEYVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGVGPRCVDYVLGIVKAYSTRVGAGPFPTELFDSVGEFLCEKGNEFGATTGRRRRTGWLDAVAVRRAVELNSLSGFCLTKLDVLDGLDEVKICVGYRLPDGREVDVTPLAAEGWEGIEPIYEVLPGWKESTFGVKQRDGLPQAALNYIKRIEEVTGVPVDIISTGPDREETIVLRHPFDA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9899 | - | - |
Protein sequence of regulator: 433 a.a. .
>REG0218 NC_013508:403538-404836 [Edwardsiella tarda EIB202]
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVVSIIANGVVLAPDAL
LREMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKESFAV
KLKEIMEYHNFQLVNYYHVDAVDYQRVLDEVMAVADLLTSMVVDVADLLNKAYRNGEYVMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGVGPRCVDYVLGIVKAYSTRVGAGPFPTELFDSVGEFLCEKGNEFGATTGRRRRTGWLDAVAVRRAV
ELNSLSGFCLTKLDVLDGLDEVKICVGYRLPDGREVDVTPLAAEGWEGIEPIYEVLPGWKESTFGVKQRDGLPQAALNYI
KRIEEVTGVPVDIISTGPDREETIVLRHPFDA*
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVVSIIANGVVLAPDAL
LREMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKESFAV
KLKEIMEYHNFQLVNYYHVDAVDYQRVLDEVMAVADLLTSMVVDVADLLNKAYRNGEYVMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGVGPRCVDYVLGIVKAYSTRVGAGPFPTELFDSVGEFLCEKGNEFGATTGRRRRTGWLDAVAVRRAV
ELNSLSGFCLTKLDVLDGLDEVKICVGYRLPDGREVDVTPLAAEGWEGIEPIYEVLPGWKESTFGVKQRDGLPQAALNYI
KRIEEVTGVPVDIISTGPDREETIVLRHPFDA*
Nucleotide sequence of regulator: 1299 bp .
>REG0218 NC_013508:403538-404836 [Edwardsiella tarda EIB202]
ATGGGCAAGAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGGTCGTAGACCTCTTAACTGAACG
GGCCAAGTATGTTGTACGCTATCAGGGCGGGCACAACGCGGGCCATACGCTAGTCATTAACGGTGAAAAAACCGTTCTGC
ATCTGATTCCCTCAGGTATTCTGCGCGAAAATGTCGTCAGTATTATTGCCAACGGCGTTGTTCTGGCGCCGGATGCGCTG
CTGCGCGAAATGACCGAGCTGGAAGCGCGCGGTGTACCGGTGCGCGAGCGCCTGCTGCTCTCTGAAGCCTGTCCGCTGAT
CCTGCCGTATCACGTTGCGCTGGACAATGCGCGAGAGAAAGCGCGCGGTGCCAAGGCTATCGGCACCACCGGTCGCGGCA
TCGGCCCGGCCTATGAAGACAAAGTCGCTCGCCGCGGCCTGCGCGTCGGCGACCTGTTTGATAAAGAAAGCTTCGCCGTT
AAGCTGAAAGAGATTATGGAATACCATAACTTCCAGCTGGTGAACTACTACCACGTCGATGCCGTTGATTACCAGCGCGT
GCTGGACGAAGTGATGGCGGTTGCCGACCTGCTGACCAGCATGGTGGTTGACGTTGCCGATCTGCTGAACAAGGCCTATC
GCAACGGCGAATATGTGATGTTCGAAGGTGCCCAGGGCACTCTGCTGGATATCGACCACGGTACCTATCCGTACGTGACC
TCCTCCAACACCACCGCCGGTGGCGTGGCGACGGGTTCCGGCGTCGGTCCGCGCTGTGTGGACTATGTGCTGGGTATCGT
GAAAGCGTACTCTACCCGCGTGGGCGCGGGTCCGTTCCCGACGGAGCTGTTCGATAGCGTCGGCGAATTCCTGTGCGAGA
AGGGCAACGAGTTTGGTGCCACCACCGGCCGTCGCCGTCGCACCGGCTGGCTGGACGCCGTTGCCGTGCGCCGCGCCGTC
GAGCTGAACTCGCTGTCTGGCTTCTGCCTGACCAAGCTGGATGTGCTGGATGGTCTGGATGAAGTGAAAATCTGCGTAGG
CTATCGCCTGCCGGATGGCCGCGAAGTCGACGTAACGCCGCTGGCTGCCGAGGGCTGGGAAGGCATTGAGCCGATCTACG
AAGTGCTGCCGGGCTGGAAAGAGAGCACCTTCGGCGTGAAGCAGCGTGACGGTCTGCCGCAGGCGGCGCTGAACTACATC
AAACGCATTGAAGAGGTTACCGGTGTACCGGTCGATATTATCTCTACCGGTCCCGACCGCGAAGAGACGATCGTTCTGCG
TCACCCGTTCGACGCGTAA
ATGGGCAAGAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGGTCGTAGACCTCTTAACTGAACG
GGCCAAGTATGTTGTACGCTATCAGGGCGGGCACAACGCGGGCCATACGCTAGTCATTAACGGTGAAAAAACCGTTCTGC
ATCTGATTCCCTCAGGTATTCTGCGCGAAAATGTCGTCAGTATTATTGCCAACGGCGTTGTTCTGGCGCCGGATGCGCTG
CTGCGCGAAATGACCGAGCTGGAAGCGCGCGGTGTACCGGTGCGCGAGCGCCTGCTGCTCTCTGAAGCCTGTCCGCTGAT
CCTGCCGTATCACGTTGCGCTGGACAATGCGCGAGAGAAAGCGCGCGGTGCCAAGGCTATCGGCACCACCGGTCGCGGCA
TCGGCCCGGCCTATGAAGACAAAGTCGCTCGCCGCGGCCTGCGCGTCGGCGACCTGTTTGATAAAGAAAGCTTCGCCGTT
AAGCTGAAAGAGATTATGGAATACCATAACTTCCAGCTGGTGAACTACTACCACGTCGATGCCGTTGATTACCAGCGCGT
GCTGGACGAAGTGATGGCGGTTGCCGACCTGCTGACCAGCATGGTGGTTGACGTTGCCGATCTGCTGAACAAGGCCTATC
GCAACGGCGAATATGTGATGTTCGAAGGTGCCCAGGGCACTCTGCTGGATATCGACCACGGTACCTATCCGTACGTGACC
TCCTCCAACACCACCGCCGGTGGCGTGGCGACGGGTTCCGGCGTCGGTCCGCGCTGTGTGGACTATGTGCTGGGTATCGT
GAAAGCGTACTCTACCCGCGTGGGCGCGGGTCCGTTCCCGACGGAGCTGTTCGATAGCGTCGGCGAATTCCTGTGCGAGA
AGGGCAACGAGTTTGGTGCCACCACCGGCCGTCGCCGTCGCACCGGCTGGCTGGACGCCGTTGCCGTGCGCCGCGCCGTC
GAGCTGAACTCGCTGTCTGGCTTCTGCCTGACCAAGCTGGATGTGCTGGATGGTCTGGATGAAGTGAAAATCTGCGTAGG
CTATCGCCTGCCGGATGGCCGCGAAGTCGACGTAACGCCGCTGGCTGCCGAGGGCTGGGAAGGCATTGAGCCGATCTACG
AAGTGCTGCCGGGCTGGAAAGAGAGCACCTTCGGCGTGAAGCAGCGTGACGGTCTGCCGCAGGCGGCGCTGAACTACATC
AAACGCATTGAAGAGGTTACCGGTGTACCGGTCGATATTATCTCTACCGGTCCCGACCGCGAAGAGACGATCGTTCTGCG
TCACCCGTTCGACGCGTAA