SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0229
Regulator type	protein
Regulatory pathway (from literatures)	TCS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Metabolic pathways (map01100); Biosynthesis of secondary metabolites (map01110); Microbial metabolism in diverse environments (map01120); Carbon metabolism (map01200); Biosynthesis of amino acids (map01230); Glycolysis / Gluconeogenesis (map00010); Fructose and mannose metabolism (map00051); Inositol phosphate metabolism (map00562); Carbon fixation in photosynthetic organisms (map00710)
Related T6SS	T6SS00372 (Type i4b)
Strain	Edwardsiella tarda EIB202
Replicon	chromosome
Sequence	Protein sequence (255 a.a.); Nucleotide sequence (765 bp)
Description	Putative activators of esrB as identified by Tn-seq.
Reference	[1] Yin K, Guan Y, Ma R, et al. Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida. PLoS Pathog. 2018 Aug 31;14(8):e1007272. doi: 10.1371/journal.ppat.1007272. eCollection 2018 Aug. PMID: 30169545

External database links

Locus tag (Gene)	ETAE_3449 (tpiA)
Coordinates (Strand)	3637381..3638145 (+)
NCBI ID	502613286
RefSeq	NC_013508
Uniprot ID	D0ZGV9_EDWTE, A0A0H3DX31_EDWTF
KEGG ID	etd:ETAF_3111, etr:ETAE_3449
KEGG pathway (determined by BlastKOALA)	map01100; map01110; map01120; map01200; map01230; map00010; map00051; map00562; map00710
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
ETAE_0886 (esrB) promoter	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
TIM	PF00121.20	3.9e-98	4..248

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-254	MRQPLVMGNWKLNGSRHMVHELVAALRGELSDVANCDVAIAPPAVYLDMAAHELAGSRIALGAQDVGINASGAFTGEISAAMLKDIGAKYIIIGHSERRTYHKEGDAFIAEKFAALKEAGLIPVLCIGETEAENEAGKTEEVCARQLDAVLKTMGAQVFKGAVIAYEPIWAIGTGKSATPEQAQAVHKFIRDHVAKHDAEAAQEVIIQYGGSVNAANAADLFKQPDIDGALVGGASLKADAFATIVRAAAAAKA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9838	-	-

Protein sequence of regulator: 255 a.a. .

                                    >REG0229 NC_013508:3637381-3638145 [Edwardsiella tarda EIB202]
MRQPLVMGNWKLNGSRHMVHELVAALRGELSDVANCDVAIAPPAVYLDMAAHELAGSRIALGAQDVGINASGAFTGEISA
AMLKDIGAKYIIIGHSERRTYHKEGDAFIAEKFAALKEAGLIPVLCIGETEAENEAGKTEEVCARQLDAVLKTMGAQVFK
GAVIAYEPIWAIGTGKSATPEQAQAVHKFIRDHVAKHDAEAAQEVIIQYGGSVNAANAADLFKQPDIDGALVGGASLKAD
AFATIVRAAAAAKA*

                                 

Nucleotide sequence of regulator: 765 bp .

                                    >REG0229 NC_013508:3637381-3638145 [Edwardsiella tarda EIB202]
ATGCGTCAACCATTAGTTATGGGTAACTGGAAACTGAACGGTAGCCGCCACATGGTTCACGAACTGGTTGCCGCCCTGCG
CGGTGAACTGAGCGACGTAGCAAACTGCGATGTCGCCATCGCCCCGCCGGCAGTCTATCTGGATATGGCCGCCCATGAAC
TGGCCGGTAGCCGTATCGCCCTCGGTGCCCAGGACGTCGGTATCAATGCCTCCGGCGCATTCACCGGTGAAATCTCCGCC
GCGATGCTGAAAGACATCGGTGCCAAATACATCATCATTGGCCACTCAGAGCGCCGTACCTACCACAAAGAAGGCGACGC
GTTCATCGCAGAGAAATTTGCCGCGCTGAAAGAAGCCGGTCTGATCCCGGTGCTGTGCATCGGTGAGACGGAAGCTGAAA
ACGAAGCCGGTAAAACGGAAGAAGTCTGCGCCCGCCAGCTGGATGCCGTACTGAAAACCATGGGTGCTCAGGTGTTTAAA
GGCGCCGTTATCGCCTATGAACCGATATGGGCCATCGGTACCGGTAAGTCTGCCACTCCGGAACAGGCTCAGGCGGTACA
CAAATTTATCCGCGATCACGTTGCCAAGCACGACGCTGAAGCCGCACAGGAAGTCATCATCCAGTACGGCGGCTCAGTCA
ACGCAGCCAATGCGGCCGATCTGTTCAAGCAGCCGGACATCGACGGTGCACTGGTCGGCGGGGCCTCTCTGAAGGCCGAC
GCATTCGCCACCATCGTACGCGCCGCCGCTGCCGCTAAAGCCTAA

                                