Detailed information of regulator
Summary
| Regulator ID | REG0298  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Metabolic pathways (map01100); Biosynthesis of amino acids (map01230); Cysteine and methionine metabolism (map00270); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Escherichia coli (map02026) |
| Related T6SS | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Description | The level of Hcp was reduced in the △cqsA and △luxS mutants where the level of the hapR expression was expected to be reduced |
| Reference |
External database links
| Locus tag (Gene) | VCA1552_RS01665 (LuxS) |
| Coordinates (Strand) | 319588..320106 (+) |
| NCBI ID | 447052971 |
| RefSeq | NZ_CP024867 |
| Uniprot ID | LUXS_VIBCH, A0A0X1L333_VIBCO, LUXS_VIBC3, A0A0K9UHI4_VIBCL, A0A0H3Q5C9_VIBCL, LUXS_VIBCM, A0A085QVK5_VIBCL |
| KEGG ID | vco:VC0395_A0091, vcq:EN18_19785, vcz:VAB027_3286, vcm:VCM66_0515, vcr:VC395_0574, vch:VC0557 |
| KEGG pathway (determined by BlastKOALA) | map01100; map01230; map00270; map02024; map05111; map02026 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| Hcp expression and secretion | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LuxS | PF02664.17 | 1.1e-58 | 4..153 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MPLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTMPNKDILSERGIHTLEHLYAGFMRNHLNGSQVEIIDISPMGCRTGFYMSLIGAPTEQQVAQAWLAAMQDVLKVESQEQIPELNEYQCGTAAMHSLEEAKAIAKNVIAAGISVNRNDELALPESMLNELKVH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9889 | - | - |
Protein sequence of regulator: 173 a.a. .
>REG0298 NZ_CP024867:319588-320106 [Vibrio cholerae]
MPLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTMPNKDILSERGIHTLEHLYAGFMRNHLNGSQVEIIDISP
MGCRTGFYMSLIGAPTEQQVAQAWLAAMQDVLKVESQEQIPELNEYQCGTAAMHSLEEAKAIAKNVIAAGISVNRNDELA
LPESMLNELKVH*
MPLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTMPNKDILSERGIHTLEHLYAGFMRNHLNGSQVEIIDISP
MGCRTGFYMSLIGAPTEQQVAQAWLAAMQDVLKVESQEQIPELNEYQCGTAAMHSLEEAKAIAKNVIAAGISVNRNDELA
LPESMLNELKVH*
Nucleotide sequence of regulator: 519 bp .
>REG0298 NZ_CP024867:319588-320106 [Vibrio cholerae]
ATGCCATTATTAGACAGTTTTACCGTCGATCATACTCGTATGAATGCACCGGCGGTGCGTGTTGCCAAAACCATGCAAAC
CCCAAAAGGGGATACGATTACCGTATTTGATTTGCGTTTTACTATGCCAAACAAAGATATCTTGTCTGAGCGCGGTATCC
ATACTCTAGAGCATCTCTACGCGGGCTTTATGCGCAATCACCTTAACGGCAGCCAAGTGGAGATCATCGATATTTCACCA
ATGGGTTGCCGTACAGGTTTCTACATGAGCTTGATTGGTGCGCCGACAGAACAGCAAGTGGCACAAGCATGGCTAGCCGC
AATGCAAGATGTGTTGAAAGTTGAAAGCCAAGAGCAAATTCCTGAGCTGAATGAGTACCAGTGCGGCACTGCGGCGATGC
ACTCGCTCGAAGAAGCCAAAGCGATTGCGAAAAACGTGATTGCGGCAGGCATCTCGGTTAACCGTAACGATGAGTTGGCG
CTGCCCGAATCTATGCTCAATGAGCTGAAGGTTCACTAA
ATGCCATTATTAGACAGTTTTACCGTCGATCATACTCGTATGAATGCACCGGCGGTGCGTGTTGCCAAAACCATGCAAAC
CCCAAAAGGGGATACGATTACCGTATTTGATTTGCGTTTTACTATGCCAAACAAAGATATCTTGTCTGAGCGCGGTATCC
ATACTCTAGAGCATCTCTACGCGGGCTTTATGCGCAATCACCTTAACGGCAGCCAAGTGGAGATCATCGATATTTCACCA
ATGGGTTGCCGTACAGGTTTCTACATGAGCTTGATTGGTGCGCCGACAGAACAGCAAGTGGCACAAGCATGGCTAGCCGC
AATGCAAGATGTGTTGAAAGTTGAAAGCCAAGAGCAAATTCCTGAGCTGAATGAGTACCAGTGCGGCACTGCGGCGATGC
ACTCGCTCGAAGAAGCCAAAGCGATTGCGAAAAACGTGATTGCGGCAGGCATCTCGGTTAACCGTAACGATGAGTTGGCG
CTGCCCGAATCTATGCTCAATGAGCTGAAGGTTCACTAA