Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00005 experimental
T6SS type Type i4a
Strain Pseudomonas aeruginosa PAO1
Replicon (RefSeq) chromosome (H3-T6SS) (NC_002516)
Location 2599893..2632231
Function anti-eukaryotic; anti-bacterial; effector translocation; virulence
Comment H3-T6SS is required for virulence in the worm model; PldB exerts an antibacterial activity and facilitates eukaryotic cells by activation of the PI3K/Akt pathway; PldB contributes to the internalization of P. aeruginosa into Human Epithelial cells.
References
[1] Kefala K et al (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
[2] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
[3] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[4] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275]
[5] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
[6] Sana TG et al (2013) Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS One. 8(10):e76030. [PudMed:24204589]
[7] Jiang F et al (2014) A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe. 15(5):600-10. [PudMed:24832454]
[8] Chen Z et al (2014) Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun. 70(Pt 7):903-5. [PudMed:25005085]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPAARSfa3TecTTssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number111111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
PA2349 2595985..2596779 + 795 NP_251039.1 hypothetical protein
PA2350 2596776..2597885 + 1110 NP_251040.1 methionine ABC transporter ATP-binding protein
PA2351 2597869..2598522 + 654 NP_251041.1 ABC transporter permease
PA2352 2598700..2599827 + 1128 NP_251042.1 glycerophosphoryl diester phosphodiesterase
PA2353 2599893..2601047 - 1155 NP_251043.1 hypothetical protein
PA2354 2601219..2602349 - 1131 NP_251044.1 transcriptional regulator
PA2355 2602346..2603530 - 1185 NP_251045.1 FMNH2-dependent monooxygenase
PA2356 (msuD) 2603560..2604705 - 1146 NP_251046.1 methanesulfonate monooxygenase
PA2357 (msuE) 2604715..2605275 - 561 NP_251047.1 FMN reductase MsuE
PA2358 2605435..2605824 - 390 NP_251048.1 hypothetical protein
T6CP012424 Sfa3 PA2359 2605937..2607022 - 1086 NP_251049.1 transcriptional regulator
T6CP000020 TssA PA2360 2607132..2608232 - 1101 NP_251050.1 hypothetical protein
T6CP000021 TssM PA2361 2608229..2612044 - 3816 NP_251051.1 hypothetical protein
T6CP008379 TssL PA2362 2612041..2612799 - 759 NP_251052.1 hypothetical protein
T6CP000022 TssK PA2363 2612817..2614148 - 1332 NP_251053.1 hypothetical protein
PA2364 2614208..2614684 - 477 NP_251054.1 hypothetical protein
T6CP000023 TssB PA2365 2614893..2615438 + 546 NP_251055.1 hypothetical protein
T6CP000024 TssC PA2366 2615461..2616945 + 1485 NP_251056.1 uricase
T6CP000025 TssD PA2367 2617019..2617516 + 498 NP_251057.1 hypothetical protein
T6CP008380 TssE PA2368 2617529..2617954 + 426 NP_251058.1 hypothetical protein
T6CP000026 TssF PA2369 2617938..2619731 + 1794 NP_251059.1 hypothetical protein
T6CP000027 TssG PA2370 2619695..2620711 + 1017 NP_251060.1 hypothetical protein
T6CP000028 TssH PA2371 2620713..2623262 + 2550 NP_251061.1 ClpA/B-type protease
T6CP000029 TssI PA2373 2624204..2626210 + 2007 NP_251063.1 hypothetical protein
PA2374 2626221..2626757 + 537 NP_251064.1 hypothetical protein
PA2375 2626780..2627175 - 396 NP_251065.1 hypothetical protein
PA2376 2627452..2628093 + 642 NP_251066.1 transcriptional regulator
PA2377 2628225..2629499 + 1275 NP_251067.1 hypothetical protein
PA2378 2629916..2632231 - 2316 NP_251068.1 aldehyde dehydrogenase
PA2379 2632228..2632698 - 471 NP_251069.1 oxidoreductase
PA2380 2632963..2633199 - 237 NP_251070.1 hypothetical protein
PA2381 2633494..2633736 + 243 NP_251071.1 hypothetical protein
PA2382 (lldA) 2633803..2634954 - 1152 NP_251072.1 L-lactate dehydrogenase
PA2383 2635051..2635971 - 921 NP_251073.1 transcriptional regulator
PA2384 2636013..2636336 - 324 NP_251074.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0018 experimental PA4462 (RpoN) protein Global regulator Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4992870..4994363 (+) 15599658 PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0070 experimental pa1663 (sfa2) protein Global regulator; QS regulatory pathway Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 1813395..1814906 (+) 15596860 PA4462 (rpoN) (↑); T6SS transcript (↑); T6SS transcript (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01466 experimental PA2374 Tie - Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2626221..2626757 (+) 15597570
EFF01482 experimental PA3907 (TseT) Tde IMU00309 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4375230..4376015 (+) 15599102
EFF01279 experimental PA5089 (PldB) Tle Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5727239..5729476 (-) 15600282
EFF01893 experimental PA2056 Tse - Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 2249693..2250595 (+) 15597252

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00044 experimental PA5086 EFF01279 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5724601..5725242 (-) 15600279
IMU00045 experimental PA5087 EFF01279 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5725479..5726348 (-) 15600280
IMU00046 experimental PA5088 EFF01279 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 5726357..5727238 (-) 15600281
IMU00309 experimental PA3908 EFF01482 experimental Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4376012..4376731 (+) 15599103

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00006 experimental PA3906 (co-TecT) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4374850..4375233 (+) 15599101 PA3905 (TecT) -
ACP00007 experimental PA3905 (tecT) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4374330..4374857 (+) 15599100 PA3904 (PAAR4) PA3907 (TseT)
ACP00008 experimental PA3790 (oprC) chaperone Pseudomonas aeruginosa PAO1 chromosome (NC_002516) 4247703..4249874 (-) 15598985 - PA4922 (Azu)