Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00005 experimental
T6SS type Type i4a
Strain Pseudomonas aeruginosa PAO1
Replicon (RefSeq) chromosome (H3-T6SS) (NC_002516)
Location 2599893..2632231
Function anti-eukaryotic; anti-bacterial; effector translocation; virulence
Comment H3-T6SS is required for virulence in the worm model; PldB exerts an antibacterial activity and facilitates eukaryotic cells by activation of the PI3K/Akt pathway; PldB contributes to the internalization of P. aeruginosa into Human Epithelial cells.
References
[1] Hachani A et al (2011) Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 286(14):12317-27. [PudMed:21325275]
[2] Kefala K et al (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 6):695-7. [PudMed:22684073]
[3] Osipiuk J et al (2011) Crystal structure of secretory protein Hcp3 from Pseudomonas aeruginosa. J Struct Funct Genomics. 12(1):21-6. [PudMed:21476004]
[4] Sana TG et al (2013) Divergent Control of Two Type VI Secretion Systems by RpoN in Pseudomonas aeruginosa. PLoS One. 8(10):e76030. [PudMed:24204589]
[5] Jiang F et al (2014) A Pseudomonas aeruginosa Type VI Secretion Phospholipase D Effector Targets Both Prokaryotic and Eukaryotic Cells. Cell Host Microbe. 15(5):600-10. [PudMed:24832454]
[6] Barret M et al (2011) Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology. 157(Pt 6):1726-39. [PudMed:21474537]
[7] Bleves S et al (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int J Med Microbiol. 300(8):534-43. [PudMed:20947426]
[8] Chen Z et al (2014) Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa. Acta Crystallogr F Struct Biol Commun. 70(Pt 7):903-5. [PudMed:25005085]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPAARSfa3TecTTssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number111111111111111


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Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
PA23492595985..2596779+795NP_251039.1hypothetical protein
PA23502596776..2597885+1110NP_251040.1methionine ABC transporter ATP-binding protein
PA23512597869..2598522+654NP_251041.1ABC transporter permease
PA23522598700..2599827+1128NP_251042.1glycerophosphoryl diester phosphodiesterase
PA23532599893..2601047-1155NP_251043.1hypothetical protein
PA23542601219..2602349-1131NP_251044.1transcriptional regulator
PA23552602346..2603530-1185NP_251045.1FMNH2-dependent monooxygenase
PA2356 (msuD)2603560..2604705-1146NP_251046.1methanesulfonate monooxygenase
PA2357 (msuE)2604715..2605275-561NP_251047.1FMN reductase MsuE
PA23582605435..2605824-390NP_251048.1hypothetical protein
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flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0018 experimentalPA4462 (RpoN)proteinGlobal regulatorPseudomonas aeruginosa PAO1chromosome (NC_002516)4992870..4994363 (+)15599658PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0070 experimentalpa1663 (sfa2)proteinGlobal regulator; QS regulatory pathwayPseudomonas aeruginosa PAO1chromosome (NC_002516)1813395..1814906 (+)15596860PA4462 (rpoN) (↑); T6SS transcript (↑); T6SS transcript (↓)
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experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Showing 1 to 4 of 4 rows

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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experimental Experimental investigation has been performed.
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Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00006 experimentalPA3906 (co-TecT)chaperonePseudomonas aeruginosa PAO1chromosome (NC_002516)4374850..4375233 (+)15599101PA3905 (TecT)-
ACP00007 experimentalPA3905 (tecT)chaperonePseudomonas aeruginosa PAO1chromosome (NC_002516)4374330..4374857 (+)15599100PA3904 (PAAR4)PA3907 (TseT)
ACP00008 experimentalPA3790 (oprC)chaperonePseudomonas aeruginosa PAO1chromosome (NC_002516)4247703..4249874 (-)15598985-PA4922 (Azu)
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