SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01893
Effector type	Tse
Cognate immunity protein	-
Related T6SS (Type)	T6SS00005 (Type i4a)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (311 a.a.); Nucleotide sequence (933 bp)
Description	Copurification experiments validated the interaction between PA2066 and Hcp2 (10-fold enrichment),PA0256 and Hcp3 (8.4-fold enrichment). PA2066 and PA0256 are bona fide Hcp cargoes and might be H2- and H3-T6SS toxins with antibacterial activity, respectively
Reference	[1] Howard SA, Furniss RCD, Bonini D, et al. The Breadth and Molecular Basis of Hcp-Driven Type VI Secretion System Effector Delivery. mBio. 2021 Jun 29;12(3):e0026221. doi: 10.1128/mBio.00262-21. Epub 2021 Jun 1. PMID: 34061601

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA0256
Coordinates (Strand)	287188..288120 (-)
NCBI ID	15595453
RefSeq	NC_002516
Uniprot ID	Q9I6N3
KEGG ID	pae:PA0256
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-310	MRSWHGVVTKIAKQGKGAVVKNRLGPEELLVPFTVSTLVEVDLDARIYGTDVDDTPWAEGDERKEVLVGTEVELLTDTDTFPTHASQVLSANFRFALDIVKGGLSGPVRKGAVLVIYHDFQQEIRRGDSGSAFAEAVVAYARNKLYKPAFAIKADTNYAQLVRDLGKVAAESGEQYFIDTILIATHGTAGQLWLGNPHGDSTADVMARRVQGKQFVEPQAFGATLLELFGKELAIALYGCDFAGDEDGEAAALELRIAANARAVYAGKGTVYLNASKEGRSTVTCSKAMVVYRTDKIEVLGGNQVPVFDI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9852	-	-

Protein sequence: 311 a.a. .

                                    >EFF01893 NC_002516:c288120-287188 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAO1]
MRSWHGVVTKIAKQGKGAVVKNRLGPEELLVPFTVSTLVEVDLDARIYGTDVDDTPWAEGDERKEVLVGTEVELLTDTDT
FPTHASQVLSANFRFALDIVKGGLSGPVRKGAVLVIYHDFQQEIRRGDSGSAFAEAVVAYARNKLYKPAFAIKADTNYAQ
LVRDLGKVAAESGEQYFIDTILIATHGTAGQLWLGNPHGDSTADVMARRVQGKQFVEPQAFGATLLELFGKELAIALYGC
DFAGDEDGEAAALELRIAANARAVYAGKGTVYLNASKEGRSTVTCSKAMVVYRTDKIEVLGGNQVPVFDI*

                                

Nucleotide sequence: 933 bp .

                                    >EFF01893 NC_002516:c288120-287188 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAO1]
ATGCGCTCATGGCATGGCGTAGTCACGAAAATCGCCAAGCAAGGCAAGGGCGCCGTGGTCAAGAATCGACTCGGCCCGGA
AGAGCTTCTGGTGCCCTTCACAGTGAGTACGCTCGTCGAGGTCGACCTGGACGCAAGGATCTACGGAACGGATGTGGACG
ACACGCCCTGGGCGGAGGGCGACGAACGAAAGGAGGTGCTGGTCGGTACCGAAGTAGAACTGCTGACCGATACGGATACC
TTTCCTACCCATGCGTCCCAGGTGCTCAGCGCCAATTTCCGTTTCGCCCTCGACATCGTCAAGGGAGGGCTCAGCGGCCC
GGTCAGGAAAGGGGCGGTCCTGGTCATCTACCACGACTTCCAGCAGGAGATCCGGAGAGGCGACTCCGGCAGCGCCTTCG
CGGAGGCGGTCGTCGCCTATGCGCGGAACAAGCTCTACAAGCCGGCCTTCGCGATCAAGGCCGATACCAACTACGCCCAG
TTGGTTCGGGATCTAGGCAAGGTTGCCGCGGAGAGCGGCGAACAATACTTCATCGATACGATCCTCATCGCCACCCACGG
CACGGCTGGCCAACTCTGGCTGGGCAACCCCCACGGCGATAGCACCGCGGACGTCATGGCCCGGCGTGTCCAGGGCAAGC
AGTTCGTCGAGCCCCAGGCCTTTGGTGCGACCCTTCTCGAACTGTTCGGCAAGGAACTGGCCATCGCCCTCTACGGGTGC
GACTTCGCCGGGGACGAGGACGGAGAAGCGGCGGCCCTCGAGCTGCGCATCGCCGCCAACGCGAGAGCGGTCTACGCCGG
CAAGGGCACGGTGTATCTCAATGCCAGCAAAGAGGGTCGTTCCACGGTGACCTGCAGCAAGGCGATGGTGGTCTACCGGA
CCGACAAGATCGAGGTACTGGGTGGAAACCAGGTACCGGTATTCGATATCTGA

                                