SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01482
Effector type	Tde
Cognate immunity protein	IMU00309
Related T6SS (Type)	T6SS00005 (Type i4a)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (262 a.a.); Nucleotide sequence (786 bp)
Description	PA3907 encodes a TOX-REase-5 effector
Reference	[1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369 [2] Wen H, Liu G, Geng Z, et al. Structure and SAXS studies unveiled a novel inhibition mechanism of the Pseudomonas aeruginosa T6SS TseT-TsiT complex. Int J Biol Macromol. 2021 Oct 1;188:450-459. doi: 10.1016/j.ijbiomac.2021.08.029. Epub 2021 Aug 8. PMID: 34371041

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA3907 (TseT)
Coordinates (Strand)	4375230..4376015 (+)
NCBI ID	15599102
RefSeq	NC_002516
Uniprot ID	Q9HXA6_PSEAE
KEGG ID	pae:PA3907
PDB ID	7DYM

Pfam domain hit(s)

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Domain	Pfam ID	E-value	Aligned region
Tox-REase-5	PF15648.8	5.4e-23	116..203

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Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction	Region	Sequence
Outside	1-261	MSGIGALFRPLPRMGPRLPFELPAPPRPHLPMPAPRPLPRPAPGALPRPVPRPMPVPQTLPRTETGTLSRDRTREAERDCSKSQGRDKDCVECPPSRGEMAIANNGKGHSMSDLSARYQQWVTNFPFPHEWFWSGTWWDGFDEPRCTLLEAKANYAFLFVPLLGVPRPWARAKVKSDLLQKAEVHSDKARPTPPVFVEWHFLQRIVYEYCAAEYLRMGLANLKAFWNPMPGTDEHDDYQETRAKEQEEMKRFCEENPGYCA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

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Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6370	-	-

Protein sequence: 262 a.a. .

                                    >EFF01482 NC_002516:4375230-4376015 Type VI DNase and RNase Effector (Tde) [Pseudomonas aeruginosa PAO1]
MSGIGALFRPLPRMGPRLPFELPAPPRPHLPMPAPRPLPRPAPGALPRPVPRPMPVPQTLPRTETGTLSRDRTREAERDC
SKSQGRDKDCVECPPSRGEMAIANNGKGHSMSDLSARYQQWVTNFPFPHEWFWSGTWWDGFDEPRCTLLEAKANYAFLFV
PLLGVPRPWARAKVKSDLLQKAEVHSDKARPTPPVFVEWHFLQRIVYEYCAAEYLRMGLANLKAFWNPMPGTDEHDDYQE
TRAKEQEEMKRFCEENPGYCA*

                                

Nucleotide sequence: 786 bp .

                                    >EFF01482 NC_002516:4375230-4376015 Type VI DNase and RNase Effector (Tde) [Pseudomonas aeruginosa PAO1]
ATGAGCGGCATAGGCGCGCTTTTCCGACCGCTGCCCCGCATGGGTCCGCGGCTGCCCTTCGAACTACCCGCGCCGCCCCG
GCCGCACTTGCCGATGCCGGCGCCGCGCCCGCTGCCGCGACCGGCCCCCGGCGCACTGCCCAGGCCGGTACCGCGTCCGA
TGCCGGTGCCGCAGACCCTGCCCAGGACCGAGACCGGCACGCTGAGCCGCGACCGGACCCGGGAAGCCGAGCGCGATTGC
AGCAAGAGCCAGGGCCGCGACAAGGACTGCGTGGAGTGCCCGCCGTCCCGGGGCGAAATGGCCATCGCCAATAACGGCAA
GGGCCACAGCATGTCGGACCTGTCCGCGCGCTACCAGCAGTGGGTGACCAATTTCCCGTTTCCCCACGAATGGTTCTGGA
GCGGCACCTGGTGGGACGGCTTCGACGAGCCGCGCTGCACCCTGCTGGAGGCGAAGGCCAACTACGCCTTCCTGTTCGTC
CCGCTGCTCGGCGTGCCCAGGCCCTGGGCACGGGCCAAGGTGAAGTCGGACCTGCTGCAGAAGGCCGAGGTCCACAGCGA
CAAGGCCCGACCGACCCCGCCGGTGTTCGTCGAATGGCACTTCCTGCAGCGGATCGTCTACGAGTACTGCGCCGCGGAGT
ACCTGCGCATGGGACTGGCCAACCTGAAGGCATTCTGGAATCCGATGCCGGGAACGGACGAGCACGACGACTACCAGGAA
ACCCGCGCGAAGGAACAGGAAGAGATGAAAAGGTTTTGCGAAGAGAACCCGGGGTATTGCGCATGA

                                