Detailed information of effector
Summary
| Effector ID | EFF01482  |
| Effector type | Tde |
| Cognate immunity protein | IMU00309 |
| Related T6SS (Type) | T6SS00005 (Type i4a) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (262 a.a.); Nucleotide sequence (786 bp) |
| Description | PA3907 encodes a TOX-REase-5 effector |
| Reference | [1] Burkinshaw BJ, Liang X, Wong M, et al. A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol. 2018 May;3(5):632-640. doi: 10.1038/s41564-018-0144-4. Epub 2018 Apr 9. PMID: 29632369 [2] Wen H, Liu G, Geng Z, et al. Structure and SAXS studies unveiled a novel inhibition mechanism of the Pseudomonas aeruginosa T6SS TseT-TsiT complex. Int J Biol Macromol. 2021 Oct 1;188:450-459. doi: 10.1016/j.ijbiomac.2021.08.029. Epub 2021 Aug 8. PMID: 34371041 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA3907 (TseT) |
| Coordinates (Strand) | 4375230..4376015 (+) |
| NCBI ID | 15599102 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9HXA6_PSEAE |
| KEGG ID | pae:PA3907 |
| PDB ID | 7DYM |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tox-REase-5 | PF15648.8 | 5.4e-23 | 116..203 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-261 | MSGIGALFRPLPRMGPRLPFELPAPPRPHLPMPAPRPLPRPAPGALPRPVPRPMPVPQTLPRTETGTLSRDRTREAERDCSKSQGRDKDCVECPPSRGEMAIANNGKGHSMSDLSARYQQWVTNFPFPHEWFWSGTWWDGFDEPRCTLLEAKANYAFLFVPLLGVPRPWARAKVKSDLLQKAEVHSDKARPTPPVFVEWHFLQRIVYEYCAAEYLRMGLANLKAFWNPMPGTDEHDDYQETRAKEQEEMKRFCEENPGYCA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.6370 | - | - |
Protein sequence: 262 a.a. .
>EFF01482 NC_002516:4375230-4376015 Type VI DNase and RNase Effector (Tde) [Pseudomonas aeruginosa PAO1]
MSGIGALFRPLPRMGPRLPFELPAPPRPHLPMPAPRPLPRPAPGALPRPVPRPMPVPQTLPRTETGTLSRDRTREAERDC
SKSQGRDKDCVECPPSRGEMAIANNGKGHSMSDLSARYQQWVTNFPFPHEWFWSGTWWDGFDEPRCTLLEAKANYAFLFV
PLLGVPRPWARAKVKSDLLQKAEVHSDKARPTPPVFVEWHFLQRIVYEYCAAEYLRMGLANLKAFWNPMPGTDEHDDYQE
TRAKEQEEMKRFCEENPGYCA*
MSGIGALFRPLPRMGPRLPFELPAPPRPHLPMPAPRPLPRPAPGALPRPVPRPMPVPQTLPRTETGTLSRDRTREAERDC
SKSQGRDKDCVECPPSRGEMAIANNGKGHSMSDLSARYQQWVTNFPFPHEWFWSGTWWDGFDEPRCTLLEAKANYAFLFV
PLLGVPRPWARAKVKSDLLQKAEVHSDKARPTPPVFVEWHFLQRIVYEYCAAEYLRMGLANLKAFWNPMPGTDEHDDYQE
TRAKEQEEMKRFCEENPGYCA*
Nucleotide sequence: 786 bp .
>EFF01482 NC_002516:4375230-4376015 Type VI DNase and RNase Effector (Tde) [Pseudomonas aeruginosa PAO1]
ATGAGCGGCATAGGCGCGCTTTTCCGACCGCTGCCCCGCATGGGTCCGCGGCTGCCCTTCGAACTACCCGCGCCGCCCCG
GCCGCACTTGCCGATGCCGGCGCCGCGCCCGCTGCCGCGACCGGCCCCCGGCGCACTGCCCAGGCCGGTACCGCGTCCGA
TGCCGGTGCCGCAGACCCTGCCCAGGACCGAGACCGGCACGCTGAGCCGCGACCGGACCCGGGAAGCCGAGCGCGATTGC
AGCAAGAGCCAGGGCCGCGACAAGGACTGCGTGGAGTGCCCGCCGTCCCGGGGCGAAATGGCCATCGCCAATAACGGCAA
GGGCCACAGCATGTCGGACCTGTCCGCGCGCTACCAGCAGTGGGTGACCAATTTCCCGTTTCCCCACGAATGGTTCTGGA
GCGGCACCTGGTGGGACGGCTTCGACGAGCCGCGCTGCACCCTGCTGGAGGCGAAGGCCAACTACGCCTTCCTGTTCGTC
CCGCTGCTCGGCGTGCCCAGGCCCTGGGCACGGGCCAAGGTGAAGTCGGACCTGCTGCAGAAGGCCGAGGTCCACAGCGA
CAAGGCCCGACCGACCCCGCCGGTGTTCGTCGAATGGCACTTCCTGCAGCGGATCGTCTACGAGTACTGCGCCGCGGAGT
ACCTGCGCATGGGACTGGCCAACCTGAAGGCATTCTGGAATCCGATGCCGGGAACGGACGAGCACGACGACTACCAGGAA
ACCCGCGCGAAGGAACAGGAAGAGATGAAAAGGTTTTGCGAAGAGAACCCGGGGTATTGCGCATGA
ATGAGCGGCATAGGCGCGCTTTTCCGACCGCTGCCCCGCATGGGTCCGCGGCTGCCCTTCGAACTACCCGCGCCGCCCCG
GCCGCACTTGCCGATGCCGGCGCCGCGCCCGCTGCCGCGACCGGCCCCCGGCGCACTGCCCAGGCCGGTACCGCGTCCGA
TGCCGGTGCCGCAGACCCTGCCCAGGACCGAGACCGGCACGCTGAGCCGCGACCGGACCCGGGAAGCCGAGCGCGATTGC
AGCAAGAGCCAGGGCCGCGACAAGGACTGCGTGGAGTGCCCGCCGTCCCGGGGCGAAATGGCCATCGCCAATAACGGCAA
GGGCCACAGCATGTCGGACCTGTCCGCGCGCTACCAGCAGTGGGTGACCAATTTCCCGTTTCCCCACGAATGGTTCTGGA
GCGGCACCTGGTGGGACGGCTTCGACGAGCCGCGCTGCACCCTGCTGGAGGCGAAGGCCAACTACGCCTTCCTGTTCGTC
CCGCTGCTCGGCGTGCCCAGGCCCTGGGCACGGGCCAAGGTGAAGTCGGACCTGCTGCAGAAGGCCGAGGTCCACAGCGA
CAAGGCCCGACCGACCCCGCCGGTGTTCGTCGAATGGCACTTCCTGCAGCGGATCGTCTACGAGTACTGCGCCGCGGAGT
ACCTGCGCATGGGACTGGCCAACCTGAAGGCATTCTGGAATCCGATGCCGGGAACGGACGAGCACGACGACTACCAGGAA
ACCCGCGCGAAGGAACAGGAAGAGATGAAAAGGTTTTGCGAAGAGAACCCGGGGTATTGCGCATGA